Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction

Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of...

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Autores: Prat-Vidal, Cristina|||0000-0002-5621-1101, Crisóstomo, Verónica|||0000-0003-1740-7029, Moscoso, Isabel|||0000-0002-6078-4666, Báez-Díaz, Claudia, Blanco-Blázquez, Virginia|||0000-0003-1971-919X, Gómez-Mauricio, Guadalupe, Albericio, Guillermo, Aguilar, Susana, Fernández-Santos, María-Eugenia|||0000-0001-9229-0022, Fernández Avilés, Francisco|||0000-0001-5501-5275, Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X, Bayés-Genís, Antoni|||0000-0002-3044-197X, Bernad, Antonio
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:302772
Acceso en línea:https://ddd.uab.cat/record/302772
https://dx.doi.org/urn:doi:10.3390/cells10102571
Access Level:acceso abierto
Palabra clave:Cardiac progenitor cell
IGF-1
HGF
Lentiviral vectors
Decellularized extracellular matrix
Porcine large animal model
Myocardial infarction
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spelling Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial InfarctionPrat-Vidal, Cristina|||0000-0002-5621-1101Crisóstomo, Verónica|||0000-0003-1740-7029Moscoso, Isabel|||0000-0002-6078-4666Báez-Díaz, ClaudiaBlanco-Blázquez, Virginia|||0000-0003-1971-919XGómez-Mauricio, GuadalupeAlbericio, GuillermoAguilar, SusanaFernández-Santos, María-Eugenia|||0000-0001-9229-0022Fernández Avilés, Francisco|||0000-0001-5501-5275Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988XBayés-Genís, Antoni|||0000-0002-3044-197XBernad, AntonioCardiac progenitor cellIGF-1HGFLentiviral vectorsDecellularized extracellular matrixPorcine large animal modelMyocardial infarctionHuman cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFP pos and HGF-mCherry pos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 10 6 /animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/302772https://dx.doi.org/urn:doi:10.3390/cells10102571reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0011/0037Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0011/0006Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00403Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00494Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-097604-B-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2017-84324-C2-1-RInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PIC18/00014Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00247open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3027722026-06-06T12:50:31Z
dc.title.none.fl_str_mv Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
title Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
spellingShingle Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
Prat-Vidal, Cristina|||0000-0002-5621-1101
Cardiac progenitor cell
IGF-1
HGF
Lentiviral vectors
Decellularized extracellular matrix
Porcine large animal model
Myocardial infarction
title_short Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
title_full Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
title_fullStr Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
title_full_unstemmed Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
title_sort Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
dc.creator.none.fl_str_mv Prat-Vidal, Cristina|||0000-0002-5621-1101
Crisóstomo, Verónica|||0000-0003-1740-7029
Moscoso, Isabel|||0000-0002-6078-4666
Báez-Díaz, Claudia
Blanco-Blázquez, Virginia|||0000-0003-1971-919X
Gómez-Mauricio, Guadalupe
Albericio, Guillermo
Aguilar, Susana
Fernández-Santos, María-Eugenia|||0000-0001-9229-0022
Fernández Avilés, Francisco|||0000-0001-5501-5275
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Bayés-Genís, Antoni|||0000-0002-3044-197X
Bernad, Antonio
author Prat-Vidal, Cristina|||0000-0002-5621-1101
author_facet Prat-Vidal, Cristina|||0000-0002-5621-1101
Crisóstomo, Verónica|||0000-0003-1740-7029
Moscoso, Isabel|||0000-0002-6078-4666
Báez-Díaz, Claudia
Blanco-Blázquez, Virginia|||0000-0003-1971-919X
Gómez-Mauricio, Guadalupe
Albericio, Guillermo
Aguilar, Susana
Fernández-Santos, María-Eugenia|||0000-0001-9229-0022
Fernández Avilés, Francisco|||0000-0001-5501-5275
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Bayés-Genís, Antoni|||0000-0002-3044-197X
Bernad, Antonio
author_role author
author2 Crisóstomo, Verónica|||0000-0003-1740-7029
Moscoso, Isabel|||0000-0002-6078-4666
Báez-Díaz, Claudia
Blanco-Blázquez, Virginia|||0000-0003-1971-919X
Gómez-Mauricio, Guadalupe
Albericio, Guillermo
Aguilar, Susana
Fernández-Santos, María-Eugenia|||0000-0001-9229-0022
Fernández Avilés, Francisco|||0000-0001-5501-5275
Sánchez Margallo, Francisco Miguel|||0000-0003-2138-988X
Bayés-Genís, Antoni|||0000-0002-3044-197X
Bernad, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cardiac progenitor cell
IGF-1
HGF
Lentiviral vectors
Decellularized extracellular matrix
Porcine large animal model
Myocardial infarction
topic Cardiac progenitor cell
IGF-1
HGF
Lentiviral vectors
Decellularized extracellular matrix
Porcine large animal model
Myocardial infarction
description Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFP pos and HGF-mCherry pos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 10 6 /animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/302772
https://dx.doi.org/urn:doi:10.3390/cells10102571
url https://ddd.uab.cat/record/302772
https://dx.doi.org/urn:doi:10.3390/cells10102571
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0011/0037
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0011/0006
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00403
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00494
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-097604-B-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2017-84324-C2-1-R
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PIC18/00014
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00247
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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