Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples

Antibiotic resistance is an increasing global problem resulting from the pressure of antibiotic usage, greater mobility of the population, and industrialization. Many antibiotic resistance genes are believed to have originated in microorganisms in the environment, and to have been transferred to oth...

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Autores: Colomer-Lluch, Marta, Jofre i Torroella, Joan, Muniesa Pérez, Ma. Teresa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/21483
Acceso en línea:https://hdl.handle.net/2445/21483
Access Level:acceso abierto
Palabra clave:Genètica bacteriana
Bacteriòfags
Antibiòtics
Bacterial genetics
Bacteriophages
Antibiotics
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spelling Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samplesColomer-Lluch, MartaJofre i Torroella, JoanMuniesa Pérez, Ma. TeresaGenètica bacterianaBacteriòfagsAntibiòticsBacterial geneticsBacteriophagesAntibioticsAntibiotic resistance is an increasing global problem resulting from the pressure of antibiotic usage, greater mobility of the population, and industrialization. Many antibiotic resistance genes are believed to have originated in microorganisms in the environment, and to have been transferred to other bacteria through mobile genetic elements. Among others, ß-lactam antibiotics show clinical efficacy and low toxicity, and they are thus widely used as antimicrobials. Resistance to ß-lactam antibiotics is conferred by ß-lactamase genes and penicillin-binding proteins, which are chromosomal- or plasmid-encoded, although there is little information available on the contribution of other mobile genetic elements, such as phages. This study is focused on three genes that confer resistance to ß-lactam antibiotics, namely two ß-lactamase genes (blaTEM and blaCTX-M9) and one encoding a penicillin-binding protein (mecA) in bacteriophage DNA isolated from environmental water samples. The three genes were quantified in the DNA isolated from bacteriophages collected from 30 urban sewage and river water samples, using quantitative PCR amplification. All three genes were detected in the DNA of phages from all the samples tested, in some cases reaching 104 gene copies (GC) of blaTEM or 102 GC of blaCTX-M and mecA. These values are consistent with the amount of fecal pollution in the sample, except for mecA, which showed a higher number of copies in river water samples than in urban sewage. The bla genes from phage DNA were transferred by electroporation to sensitive host bacteria, which became resistant to ampicillin. blaTEM and blaCTX were detected in the DNA of the resistant clones after transfection. This study indicates that phages are reservoirs of resistance genes in the environment.Pone2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/21483Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0017549PLoS ONE, 2011, 6(3), paper e17549http://dx.doi.org/10.1371/journal.pone.0017549cc-by, (c) Colomer et al., 2011http://creativecommons.org/licenses/by/2.5/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/214832026-05-27T06:46:51Z
dc.title.none.fl_str_mv Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
title Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
spellingShingle Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
Colomer-Lluch, Marta
Genètica bacteriana
Bacteriòfags
Antibiòtics
Bacterial genetics
Bacteriophages
Antibiotics
title_short Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
title_full Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
title_fullStr Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
title_full_unstemmed Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
title_sort Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples
dc.creator.none.fl_str_mv Colomer-Lluch, Marta
Jofre i Torroella, Joan
Muniesa Pérez, Ma. Teresa
author Colomer-Lluch, Marta
author_facet Colomer-Lluch, Marta
Jofre i Torroella, Joan
Muniesa Pérez, Ma. Teresa
author_role author
author2 Jofre i Torroella, Joan
Muniesa Pérez, Ma. Teresa
author2_role author
author
dc.subject.none.fl_str_mv Genètica bacteriana
Bacteriòfags
Antibiòtics
Bacterial genetics
Bacteriophages
Antibiotics
topic Genètica bacteriana
Bacteriòfags
Antibiòtics
Bacterial genetics
Bacteriophages
Antibiotics
description Antibiotic resistance is an increasing global problem resulting from the pressure of antibiotic usage, greater mobility of the population, and industrialization. Many antibiotic resistance genes are believed to have originated in microorganisms in the environment, and to have been transferred to other bacteria through mobile genetic elements. Among others, ß-lactam antibiotics show clinical efficacy and low toxicity, and they are thus widely used as antimicrobials. Resistance to ß-lactam antibiotics is conferred by ß-lactamase genes and penicillin-binding proteins, which are chromosomal- or plasmid-encoded, although there is little information available on the contribution of other mobile genetic elements, such as phages. This study is focused on three genes that confer resistance to ß-lactam antibiotics, namely two ß-lactamase genes (blaTEM and blaCTX-M9) and one encoding a penicillin-binding protein (mecA) in bacteriophage DNA isolated from environmental water samples. The three genes were quantified in the DNA isolated from bacteriophages collected from 30 urban sewage and river water samples, using quantitative PCR amplification. All three genes were detected in the DNA of phages from all the samples tested, in some cases reaching 104 gene copies (GC) of blaTEM or 102 GC of blaCTX-M and mecA. These values are consistent with the amount of fecal pollution in the sample, except for mecA, which showed a higher number of copies in river water samples than in urban sewage. The bla genes from phage DNA were transferred by electroporation to sensitive host bacteria, which became resistant to ampicillin. blaTEM and blaCTX were detected in the DNA of the resistant clones after transfection. This study indicates that phages are reservoirs of resistance genes in the environment.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/21483
url https://hdl.handle.net/2445/21483
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0017549
PLoS ONE, 2011, 6(3), paper e17549
http://dx.doi.org/10.1371/journal.pone.0017549
dc.rights.none.fl_str_mv cc-by, (c) Colomer et al., 2011
http://creativecommons.org/licenses/by/2.5/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by, (c) Colomer et al., 2011
http://creativecommons.org/licenses/by/2.5/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pone
publisher.none.fl_str_mv Pone
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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