circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p

Background: As a type of non-coding RNA, circular RNAs (circRNAs) are considered to be functional molecules associated with human cancers. An increasing number of circRNAs have been verified in malignant progression in a number of cancers. The circRNA, circFBXW7, has been proven to play an important...

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Autores: Dong, Yanting, Qiu, Tong, Xuan, Yunpeng, Liu, Ao, Sun, Xiao, Huang, Zhangfeng, Su, Wenhao, Du, Wenxing, Yun, Tianxiang, Wo, Yang, Navarro Ponz, Alfons, Jiao, Wenjie
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183743
Acceso en línea:https://hdl.handle.net/2445/183743
Access Level:acceso abierto
Palabra clave:Càncer de pulmó
Carcinogènesi
Lung cancer
Carcinogenesis
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repository_id_str
spelling circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5pDong, YantingQiu, TongXuan, YunpengLiu, AoSun, XiaoHuang, ZhangfengSu, WenhaoDu, WenxingYun, TianxiangWo, YangNavarro Ponz, AlfonsJiao, WenjieCàncer de pulmóCarcinogènesiLung cancerCarcinogenesisBackground: As a type of non-coding RNA, circular RNAs (circRNAs) are considered to be functional molecules associated with human cancers. An increasing number of circRNAs have been verified in malignant progression in a number of cancers. The circRNA, circFBXW7, has been proven to play an important role in tumor proliferation and metastasis. However, whether circFBXW7 influences progression in lung adenocarcinoma (LUAD) remains unclear. Methods: Quantitative real-time reverse transcriptase PCR (qRT-PCR) was used to verify circFBXW7 in LUAD cell lines and LUAD tissues. Kaplan-Meier analysis was then used to compare the disease-free survival (DFS) and overall survival (OS) of these LUAD patients. The biological function of circFBXW7 was examined by overexpression and knockdown of circFBXW7 using MTT assay, EdU assay, wound-healing assay, and Transwell in vitro assays. To explore the mechanism of the circFBXW7, RNA pull-down assay, dual luciferase reporter assay, and RNA immunoprecipitation (RIP) assay were employed to examine the interaction between circFBXW7 and miR-942-5p. Western blot was used to study the fundamental proteins associated with the epithelial-mesenchymal transition (EMT) pathway. In vivo studies with BALB/c nude mice subcutaneously injected with cells stably overexpressing circFBXW7 were performed to further validate the in vitro results. Results: circFBXW7 was downregulated in LUAD cell lines and tissues, and LUAD patients with lower levels had shorter DFS and OS. The in vitro study showed that circFBXW7 overexpression inhibited proliferation and migration of A549 and HCC2279 cell lines. These results were confirmed by circFBXW7 knockdown, which showed the reverse effect. The in vivo model showed that the circRNA levels influenced the tumor growth. Finally, we determined that circFBXW7 target miRNA-942-5p which regulates the EMT gene BARX2. The modulation of circFBXW7 levels produced significant changes in EMT genes in vitro and in vivo. Conclusions: Our findings showed that circFBXW7 inhibits proliferation and migration by controlling the miR-942-5p/BARX2 axis in LUAD cell lines and its levels correlates with patient survival suggesting that regulating circFBXW7 could have therapeutic value in treating LUAD patients.AME Publishing Company2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17 p.application/pdfhttps://hdl.handle.net/2445/183743Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.21037/tlcr-21-230Translational Lung Cancer Research, 2021, vol. 10, num. 3, p. 1457-1473https://doi.org/10.21037/tlcr-21-230cc-by-nc-nd (c) AME Publishing Company, 2021https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1837432026-05-29T05:05:01Z
dc.title.none.fl_str_mv circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
title circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
spellingShingle circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
Dong, Yanting
Càncer de pulmó
Carcinogènesi
Lung cancer
Carcinogenesis
title_short circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
title_full circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
title_fullStr circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
title_full_unstemmed circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
title_sort circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p
dc.creator.none.fl_str_mv Dong, Yanting
Qiu, Tong
Xuan, Yunpeng
Liu, Ao
Sun, Xiao
Huang, Zhangfeng
Su, Wenhao
Du, Wenxing
Yun, Tianxiang
Wo, Yang
Navarro Ponz, Alfons
Jiao, Wenjie
author Dong, Yanting
author_facet Dong, Yanting
Qiu, Tong
Xuan, Yunpeng
Liu, Ao
Sun, Xiao
Huang, Zhangfeng
Su, Wenhao
Du, Wenxing
Yun, Tianxiang
Wo, Yang
Navarro Ponz, Alfons
Jiao, Wenjie
author_role author
author2 Qiu, Tong
Xuan, Yunpeng
Liu, Ao
Sun, Xiao
Huang, Zhangfeng
Su, Wenhao
Du, Wenxing
Yun, Tianxiang
Wo, Yang
Navarro Ponz, Alfons
Jiao, Wenjie
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de pulmó
Carcinogènesi
Lung cancer
Carcinogenesis
topic Càncer de pulmó
Carcinogènesi
Lung cancer
Carcinogenesis
description Background: As a type of non-coding RNA, circular RNAs (circRNAs) are considered to be functional molecules associated with human cancers. An increasing number of circRNAs have been verified in malignant progression in a number of cancers. The circRNA, circFBXW7, has been proven to play an important role in tumor proliferation and metastasis. However, whether circFBXW7 influences progression in lung adenocarcinoma (LUAD) remains unclear. Methods: Quantitative real-time reverse transcriptase PCR (qRT-PCR) was used to verify circFBXW7 in LUAD cell lines and LUAD tissues. Kaplan-Meier analysis was then used to compare the disease-free survival (DFS) and overall survival (OS) of these LUAD patients. The biological function of circFBXW7 was examined by overexpression and knockdown of circFBXW7 using MTT assay, EdU assay, wound-healing assay, and Transwell in vitro assays. To explore the mechanism of the circFBXW7, RNA pull-down assay, dual luciferase reporter assay, and RNA immunoprecipitation (RIP) assay were employed to examine the interaction between circFBXW7 and miR-942-5p. Western blot was used to study the fundamental proteins associated with the epithelial-mesenchymal transition (EMT) pathway. In vivo studies with BALB/c nude mice subcutaneously injected with cells stably overexpressing circFBXW7 were performed to further validate the in vitro results. Results: circFBXW7 was downregulated in LUAD cell lines and tissues, and LUAD patients with lower levels had shorter DFS and OS. The in vitro study showed that circFBXW7 overexpression inhibited proliferation and migration of A549 and HCC2279 cell lines. These results were confirmed by circFBXW7 knockdown, which showed the reverse effect. The in vivo model showed that the circRNA levels influenced the tumor growth. Finally, we determined that circFBXW7 target miRNA-942-5p which regulates the EMT gene BARX2. The modulation of circFBXW7 levels produced significant changes in EMT genes in vitro and in vivo. Conclusions: Our findings showed that circFBXW7 inhibits proliferation and migration by controlling the miR-942-5p/BARX2 axis in LUAD cell lines and its levels correlates with patient survival suggesting that regulating circFBXW7 could have therapeutic value in treating LUAD patients.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183743
url https://hdl.handle.net/2445/183743
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.21037/tlcr-21-230
Translational Lung Cancer Research, 2021, vol. 10, num. 3, p. 1457-1473
https://doi.org/10.21037/tlcr-21-230
dc.rights.none.fl_str_mv cc-by-nc-nd (c) AME Publishing Company, 2021
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) AME Publishing Company, 2021
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17 p.
application/pdf
dc.publisher.none.fl_str_mv AME Publishing Company
publisher.none.fl_str_mv AME Publishing Company
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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