Highly stable holographic dumbbel combined with accurate force measurements through back-focal-plane interferometry
English: Optical trapping experiments with multiple traps have been used to measure small net movements on the nanometer range, but at present more stability is demanded to measure even smaller scales which are typical of many biological motions. The main problem of these systems is that the degree...
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| Tipo de documento: | dissertação |
| Data de publicação: | 2011 |
| País: | España |
| Recursos: | Universitat Politècnica de Catalunya (UPC) |
| Repositório: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglês |
| OAI Identifier: | oai:upcommons.upc.edu:2099.1/12956 |
| Acesso em linha: | https://hdl.handle.net/2099.1/12956 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Biomechanics Interferometry Optical tweezers Holography Dumbbell Back-focal-plane interferometry pinzas ópticas Holografía Interferometría en el plano focal Bioenginyeria -- Instrumentació Òptica Biomecànica Interferometria Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomecànica Àrees temàtiques de la UPC::Enginyeria de la telecomunicació::Processament del senyal::Reconeixement de formes |
| Resumo: | English: Optical trapping experiments with multiple traps have been used to measure small net movements on the nanometer range, but at present more stability is demanded to measure even smaller scales which are typical of many biological motions. The main problem of these systems is that the degree traps are affected by perturbations can vary from one trap to the other due to differences in the optical path they follow. In holographic optical tweezers the traps share the same optical path and hence stability should be enhanced. However, holographic systems are weak regarding force measurement when multiple traps are used. Here, we test a method that allows measuring forces through back-focal-plane interferometry when multiple holographic traps are in use, and compare the stability of dumbbells created by a holographic system or by a two split beams configuration, which is currently the standard setup in experiments in which precision is a concern. |
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