Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers

Pelophylax perezi is an Iberian green waterfrog with high tolerance to habitat alteration that at times shows local population growth and demographic expansion, even where other species decline. However, pond destruction, invasive predators and hybridisation with other European waterfrog species (P....

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Autores: Sánchez-Montes, Gregorio, Recuero, Ernesto, Gutiérrez-Rodríguez, Jorge, Gómez-Mestre, Iván, Martínez-Solano, Íñigo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/132874
Acceso en línea:http://hdl.handle.net/10261/132874
Access Level:acceso abierto
Palabra clave:Pelophylax perezi
Pelophylax ridibundus
Pelophylax kl. grafi
Microsatellites
Cross-amplification
Hybridisation
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spelling Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markersSánchez-Montes, GregorioRecuero, ErnestoGutiérrez-Rodríguez, JorgeGómez-Mestre, IvánMartínez-Solano, ÍñigoPelophylax pereziPelophylax ridibundusPelophylax kl. grafiMicrosatellitesCross-amplificationHybridisationPelophylax perezi is an Iberian green waterfrog with high tolerance to habitat alteration that at times shows local population growth and demographic expansion, even where other species decline. However, pond destruction, invasive predators and hybridisation with other European waterfrog species (P. ridibundus) threaten many of its populations across its range. Hybrids of P. perezi and P. ridibundus (P. kl. grafi) can breed successfully with the former parental species after discarding the whole P. perezi genome in the germinal line, thus representing a sexual parasite for P. perezi. However, little is known about the extent of the contact zone of this hybridogenetic complex. Due to the morphological similarity of the three taxa, molecular tools are needed to delineate their respective ranges. Here we characterise a set of 16 microsatellite markers specifically developed for P. perezi. These markers showed moderate to high polymorphism (2–17 alleles/locus) in two populations from central Spain (n=20 and n=23), allowing individual identification of frogs. Seven of these markers cross-amplified in individuals of P. ridibundus from southern France (3–8 alleles/locus). These markers were used to genotype samples along a transect from southern France to eastern Spain, encompassing both pure and hybrid individuals. Sample assignment to each taxon was based on the new microsatellite loci and compared with nuclear and mitochondrial sequence data. Our results show that these markers are useful to distinguish P. ridibundus, P. pereziand the hybrid form P. kl. grafi from each other, even when sample sizes are low. The newly characterised markers will also be useful in demographic and phylogeographic studies in P. perezi and are thus a valuable tool for evolutionary and conservation oriented researchThis research was funded by grants CGL2008-04271-C02- 01/BOS, and CGL2011-28300 (Ministerio de Ciencia e Innovación- MICINN), Ministerio de Economía y Competitividad- MEC, Spain, and FEDER). G. Sánchez-Montes is funded by a predoctoral grant provided by the Asociación de Amigos de la Universidad de Navarra. E. Recuero is supported by a DGAPA-UNAM postdoctoral fellowship. J. Gutiérrez-Rodríguez is supported by the Consejo Superior de Investigaciones Científicas of Spain (CSIC) and the European Social Fund (ESF) (JAE-pre PhD fellowship). IMS was funded by the project ‘Biodiversity, Ecology and Global Change’, co-financed by North Portugal Regional Operational Programme 2007/2013 (ON.2–O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) and is currently supported by funding from the Spanish Severo Ochoa Program (SEV- 2012-0262).Peer reviewedBritish Herpetological SocietyMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Asociación de Amigos de la Universidad de NavarraUniversidad Pública de NavarraConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/132874reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.thebhs.org/index.php?option=com_docman&task=cat_view&gid=127&Itemid=42Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1328742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
title Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
spellingShingle Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
Sánchez-Montes, Gregorio
Pelophylax perezi
Pelophylax ridibundus
Pelophylax kl. grafi
Microsatellites
Cross-amplification
Hybridisation
title_short Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
title_full Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
title_fullStr Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
title_full_unstemmed Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
title_sort Species assignment in the pelophylax ridibundus x P. perezi hybridogenetic complex based on 16 newly characterised microsatellite markers
dc.creator.none.fl_str_mv Sánchez-Montes, Gregorio
Recuero, Ernesto
Gutiérrez-Rodríguez, Jorge
Gómez-Mestre, Iván
Martínez-Solano, Íñigo
author Sánchez-Montes, Gregorio
author_facet Sánchez-Montes, Gregorio
Recuero, Ernesto
Gutiérrez-Rodríguez, Jorge
Gómez-Mestre, Iván
Martínez-Solano, Íñigo
author_role author
author2 Recuero, Ernesto
Gutiérrez-Rodríguez, Jorge
Gómez-Mestre, Iván
Martínez-Solano, Íñigo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Asociación de Amigos de la Universidad de Navarra
Universidad Pública de Navarra
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pelophylax perezi
Pelophylax ridibundus
Pelophylax kl. grafi
Microsatellites
Cross-amplification
Hybridisation
topic Pelophylax perezi
Pelophylax ridibundus
Pelophylax kl. grafi
Microsatellites
Cross-amplification
Hybridisation
description Pelophylax perezi is an Iberian green waterfrog with high tolerance to habitat alteration that at times shows local population growth and demographic expansion, even where other species decline. However, pond destruction, invasive predators and hybridisation with other European waterfrog species (P. ridibundus) threaten many of its populations across its range. Hybrids of P. perezi and P. ridibundus (P. kl. grafi) can breed successfully with the former parental species after discarding the whole P. perezi genome in the germinal line, thus representing a sexual parasite for P. perezi. However, little is known about the extent of the contact zone of this hybridogenetic complex. Due to the morphological similarity of the three taxa, molecular tools are needed to delineate their respective ranges. Here we characterise a set of 16 microsatellite markers specifically developed for P. perezi. These markers showed moderate to high polymorphism (2–17 alleles/locus) in two populations from central Spain (n=20 and n=23), allowing individual identification of frogs. Seven of these markers cross-amplified in individuals of P. ridibundus from southern France (3–8 alleles/locus). These markers were used to genotype samples along a transect from southern France to eastern Spain, encompassing both pure and hybrid individuals. Sample assignment to each taxon was based on the new microsatellite loci and compared with nuclear and mitochondrial sequence data. Our results show that these markers are useful to distinguish P. ridibundus, P. pereziand the hybrid form P. kl. grafi from each other, even when sample sizes are low. The newly characterised markers will also be useful in demographic and phylogeographic studies in P. perezi and are thus a valuable tool for evolutionary and conservation oriented research
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/132874
url http://hdl.handle.net/10261/132874
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.thebhs.org/index.php?option=com_docman&task=cat_view&gid=127&Itemid=42
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv British Herpetological Society
publisher.none.fl_str_mv British Herpetological Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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