Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization

The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a b...

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Autores: Rivera-Tovar, Pamela Raquel, Contreras-Contreras, Gabriela, Rivas-Reyes, Paulina Isabel, Pérez-Jiménez, Jara, Martínez-Cifuentes, Maximiliano, Pérez-Correa, José Ricardo, Mariotti-Celis, María Salomé
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:dnet:minerva_____::e612c72e9479de5ac739fb7691813d44
Acceso en línea:https://hdl.handle.net/10347/47534
Access Level:acceso abierto
Palabra clave:Brown seaweed
Integrated extraction–purification processes
Mass balance
Spectrophotometric
UHPLC-QToF MS/MS
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spelling Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced CharacterizationRivera-Tovar, Pamela RaquelContreras-Contreras, GabrielaRivas-Reyes, Paulina IsabelPérez-Jiménez, JaraMartínez-Cifuentes, MaximilianoPérez-Correa, José RicardoMariotti-Celis, María SaloméBrown seaweedIntegrated extraction–purification processesMass balanceSpectrophotometricUHPLC-QToF MS/MSThe rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.MDPIUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía20252025-02-2120252025-02-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/47534reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:minerva_____::e612c72e9479de5ac739fb7691813d442026-06-15T12:47:27Z
dc.title.none.fl_str_mv Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
title Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
spellingShingle Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
Rivera-Tovar, Pamela Raquel
Brown seaweed
Integrated extraction–purification processes
Mass balance
Spectrophotometric
UHPLC-QToF MS/MS
title_short Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
title_full Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
title_fullStr Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
title_full_unstemmed Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
title_sort Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization
dc.creator.none.fl_str_mv Rivera-Tovar, Pamela Raquel
Contreras-Contreras, Gabriela
Rivas-Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez-Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author Rivera-Tovar, Pamela Raquel
author_facet Rivera-Tovar, Pamela Raquel
Contreras-Contreras, Gabriela
Rivas-Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez-Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author_role author
author2 Contreras-Contreras, Gabriela
Rivas-Reyes, Paulina Isabel
Pérez-Jiménez, Jara
Martínez-Cifuentes, Maximiliano
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.subject.none.fl_str_mv Brown seaweed
Integrated extraction–purification processes
Mass balance
Spectrophotometric
UHPLC-QToF MS/MS
topic Brown seaweed
Integrated extraction–purification processes
Mass balance
Spectrophotometric
UHPLC-QToF MS/MS
description The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-02-21
2025
2025-02-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/47534
url https://hdl.handle.net/10347/47534
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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