Exploiting sequence and stability information for directing nanobody stability engineering

[Background] Variable domains of camelid heavy-chain antibodies, commonly named nanobodies, have highbiotechnological potential. In view of their broad range of applications in research, diagnostics and therapy,engineering their stability is of particular interest. One important aspect is the improv...

Descripción completa

Detalles Bibliográficos
Autores: Kunz, Patrick, Flock, Tilman, Soler, Nicolas, Zaiss, Moritz, Vincke, Cécile, Sterckx, Yann, Kastelic, Damjana, Muyldermans, Serge, Hoheisel, Jörg D.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/174508
Acceso en línea:http://hdl.handle.net/10261/174508
Access Level:acceso abierto
Palabra clave:Single-domain antibody (sdAb, nanobody)
Protein aggregation
Protein design
Protein engineering
Protein stability
Descripción
Sumario:[Background] Variable domains of camelid heavy-chain antibodies, commonly named nanobodies, have highbiotechnological potential. In view of their broad range of applications in research, diagnostics and therapy,engineering their stability is of particular interest. One important aspect is the improvement of thermostability,because it can have immediate effects on conformational stability, protease resistance and aggregation pro-pensity of the protein

Similares en LA Referencia