Warm temperature modifies cell fates to reduce stomata production in Arabidopsis
Stomatal abundance decrease in Arabidopsis triggered by warm temperature is attributedto PIF4-mediated repression of SPEECHLESS (SPCH) expression. We identi?ed the unknowndevelopmental and transcriptional basis of this adaptive response. We traced stomatal lineages in vivo using cell-identity marker...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Fundación Dialnet. Universidad de La Rioja |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/46529 |
| Acceso en línea: | https://hdl.handle.net/10578/46529 |
| Access Level: | acceso abierto |
| Palabra clave: | Cell fate MUTE PIF4 SPCH Stomatal lineage development Thermomorphogenesis |
| Sumario: | Stomatal abundance decrease in Arabidopsis triggered by warm temperature is attributedto PIF4-mediated repression of SPEECHLESS (SPCH) expression. We identi?ed the unknowndevelopmental and transcriptional basis of this adaptive response. We traced stomatal lineages in vivo using cell-identity marker lines and mutants, quanti?edepidermal traits, and conducted RNA sequencing under oscillating temperatures. Prolonged warm temperature or PIF4-overexpression altered cell fates, inducing diverted sto-matal precursors (DPs) that lacked stomatal fate, contributing to stomata reduction. DPs origi-nated from meristemoids that lost SPCH expression, lacked MUTE expression, and exited the cellcycle. Short warm-temperature pulses allowed later recovery of SPCH expression and did notinduce DPs or stomata reduction. Comparison of transcriptomes obtained duringwarm-temperature pulses with stomatal lineage cell-speci?c pro?les identi?ed gene expressionchanges and contrasted their reversibility. Though at warm temperatures, key stomatal driverswere downregulated, most lineages formed stomata through partly modi?ed transcriptionallandscapes that promoted uncommitted cell identities and could include noncanonical pathways. Expression changes in stomatal regulators and cell-fate changes explain lineage progressionunder ?uctuating temperatures. Since short-term temperature oscillations prevail in naturalconditions, the requirement of long warm-temperature exposure to trigger DPs would pre-vent stomata reduction by occasional temperature rises. Promoting uncommitted lineagestages provides ?exibility to stomatal development under environmental changes. |
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