Tryptophan levels as a marker of auxins and nitric oxide signaling

The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to pe...

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Autores: López Gómez, Pedro, Smith, Edward N., Bota, Pedro, Cornejo Ibergallartu, Alfonso, Urra, Marina, Buezo Bravo, Javier, Morán Juez, José Fernando
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/43020
Acceso en línea:https://hdl.handle.net/2454/43020
Access Level:acceso abierto
Palabra clave:Tryptophan
Indole-3-acetaldoxime
IAOx
Nitric oxide
NO
Stress
Principal-component analysis
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spelling Tryptophan levels as a marker of auxins and nitric oxide signalingLópez Gómez, PedroSmith, Edward N.Bota, PedroCornejo Ibergallartu, AlfonsoUrra, MarinaBuezo Bravo, JavierMorán Juez, José FernandoTryptophanIndole-3-acetaldoximeIAOxNitric oxideNOStressPrincipal-component analysisThe aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass-identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling.This work was supported by grants from the Spanish Ministry of Science and Innovation (AGL2017-52396-P, co-financed by the European Regional Development Fund, FEDER) and a grant from the Public University of Navarre (Res 309/2022) to J.F.M. M.U. is a recipient of a pre-doctoral fellowship from the Government of Navarre, Spain. J.B. and P.L. have received pre-doctoral fellow-ships from the Public University of Navarre, Spain. J.B. was supported by the Romanian Ministry of Education and research, grant CNCS-UEFISCDI, project number PN-III-P4-ID-PCE-2020-2696, within PNCDI III.MDPIZientziakInstitute for Advanced Materials and Mathematics - INAMAT2Institute for Multidisciplinary Research in Applied Biology - IMABCienciasGobierno de Navarra / Nafarroako GobernuaUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/43020reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-52396-P© 2022 by the authors. Creative Commons Attribution 4.0https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/430202026-06-17T12:41:47Z
dc.title.none.fl_str_mv Tryptophan levels as a marker of auxins and nitric oxide signaling
title Tryptophan levels as a marker of auxins and nitric oxide signaling
spellingShingle Tryptophan levels as a marker of auxins and nitric oxide signaling
López Gómez, Pedro
Tryptophan
Indole-3-acetaldoxime
IAOx
Nitric oxide
NO
Stress
Principal-component analysis
title_short Tryptophan levels as a marker of auxins and nitric oxide signaling
title_full Tryptophan levels as a marker of auxins and nitric oxide signaling
title_fullStr Tryptophan levels as a marker of auxins and nitric oxide signaling
title_full_unstemmed Tryptophan levels as a marker of auxins and nitric oxide signaling
title_sort Tryptophan levels as a marker of auxins and nitric oxide signaling
dc.creator.none.fl_str_mv López Gómez, Pedro
Smith, Edward N.
Bota, Pedro
Cornejo Ibergallartu, Alfonso
Urra, Marina
Buezo Bravo, Javier
Morán Juez, José Fernando
author López Gómez, Pedro
author_facet López Gómez, Pedro
Smith, Edward N.
Bota, Pedro
Cornejo Ibergallartu, Alfonso
Urra, Marina
Buezo Bravo, Javier
Morán Juez, José Fernando
author_role author
author2 Smith, Edward N.
Bota, Pedro
Cornejo Ibergallartu, Alfonso
Urra, Marina
Buezo Bravo, Javier
Morán Juez, José Fernando
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Zientziak
Institute for Advanced Materials and Mathematics - INAMAT2
Institute for Multidisciplinary Research in Applied Biology - IMAB
Ciencias
Gobierno de Navarra / Nafarroako Gobernua
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
dc.subject.none.fl_str_mv Tryptophan
Indole-3-acetaldoxime
IAOx
Nitric oxide
NO
Stress
Principal-component analysis
topic Tryptophan
Indole-3-acetaldoxime
IAOx
Nitric oxide
NO
Stress
Principal-component analysis
description The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass-identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/43020
url https://hdl.handle.net/2454/43020
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-52396-P
dc.rights.none.fl_str_mv © 2022 by the authors. Creative Commons Attribution 4.0
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2022 by the authors. Creative Commons Attribution 4.0
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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