Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria

Conventional assays to measure phosphorus in freshwater systems are sometimes not sufficient to quantify the actual bioavailable P for aquatic biota since some inorganic or organic P species may not be detected by chemical methods, and their bioavailability can be affected by a range of environmenta...

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Autores: Muñoz Martín, María Ángeles, Martínez-Rosell, Aitor, Mateo Ortega, Pilar, Perona Urízar, Elvira Victoria, Fernández Piñas, Francisca
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/717425
Acceso en línea:http://hdl.handle.net/10486/717425
https://dx.doi.org/10.1016/j.scitotenv.2013.06.076
Access Level:acceso abierto
Palabra clave:Bioavailable phosphorus
calyptra
hairs
phosphatase activity
polyphosphate granules
self-luminescent bioreporter
Biología y Biomedicina / Biología
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spelling Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteriaMuñoz Martín, María ÁngelesMartínez-Rosell, AitorMateo Ortega, PilarPerona Urízar, Elvira VictoriaFernández Piñas, FranciscaBioavailable phosphoruscalyptrahairsphosphatase activitypolyphosphate granulesself-luminescent bioreporterBiología y Biomedicina / BiologíaConventional assays to measure phosphorus in freshwater systems are sometimes not sufficient to quantify the actual bioavailable P for aquatic biota since some inorganic or organic P species may not be detected by chemical methods, and their bioavailability can be affected by a range of environmental factors. This situation could lead regulatory agencies to be unable to detect imminent ecosystem-degrading phenomena such as cyanobacterial blooms. It could also be an obstacle in studying the ecophysiological requirements of freshwater communities. P bioavailability in five rivers located in central Spain was analysed by a polyphasic approach (combinations of different marker types) based on cyanobacteria. This approach included a parallel study with the use of a self-luminescent P-cyanobacterial bioreporter based on a phosphatase alkaline promoter, determination of in situ alkaline phosphatase activities from cyanobacteria found at sampling sites, and the characterisation of cyanobacterial morphological features related to P bioavailability (hairs, polyphosphate granules and calyptras). An inverse relationship was found between values of bioavailable P, measured by the bioreporter and phosphatase activities. Cyanobacteria from sampling sites with low bioavailable P showed high phosphatase activity and vice versa, although some differences in values of this activity were observed in different cyanobacteria found at the same place, in relation to different growth strategies. Morphological characteristics associated with P limitation or P enrichment also varied between sampling locations. Cyanobacteria collected from sampling sites with reduced P bioavailability, measured by bioreporter and phosphatase activity, had a lower abundance of polyphosphate granules; those cyanobacteria capable of developing hairs or calyptras showed a greater abundance of these structures. Conversely, polyphosphate granules in cyanobacteria increased as P bioavailability increased as measured by the bioreporter and phosphatase activity. The study shows that the results of genetic, physiological and microscopic analyses based on these methods complement each other, implying that combining their findings would provide a more complete analysis of the nutrient status of running watersThis work was funded by Comunidad de Madrid grants S-0505/AMB/0321 and S-2009/AMB/1511 (Microambiente-CM) and by the Spanish Ministry of Science and Innovation ( CGL2008-02397/BOS , and CGL2010-15675/BOS ). M. Angeles Muñoz-Martín is a recipient of a postdoctoral contract from the Comunidad de Madrid, Spain.ElsevierDepartamento de BiologíaFacultad de Ciencias20142014-03-15research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/717425https://dx.doi.org/10.1016/j.scitotenv.2013.06.076reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7174252026-06-23T12:46:27Z
dc.title.none.fl_str_mv Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
title Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
spellingShingle Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
Muñoz Martín, María Ángeles
Bioavailable phosphorus
calyptra
hairs
phosphatase activity
polyphosphate granules
self-luminescent bioreporter
Biología y Biomedicina / Biología
title_short Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
title_full Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
title_fullStr Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
title_full_unstemmed Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
title_sort Monitoring bioavailable phosphorus in lotic systems: A polyphasic approach based on cyanobacteria
dc.creator.none.fl_str_mv Muñoz Martín, María Ángeles
Martínez-Rosell, Aitor
Mateo Ortega, Pilar
Perona Urízar, Elvira Victoria
Fernández Piñas, Francisca
author Muñoz Martín, María Ángeles
author_facet Muñoz Martín, María Ángeles
Martínez-Rosell, Aitor
Mateo Ortega, Pilar
Perona Urízar, Elvira Victoria
Fernández Piñas, Francisca
author_role author
author2 Martínez-Rosell, Aitor
Mateo Ortega, Pilar
Perona Urízar, Elvira Victoria
Fernández Piñas, Francisca
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Bioavailable phosphorus
calyptra
hairs
phosphatase activity
polyphosphate granules
self-luminescent bioreporter
Biología y Biomedicina / Biología
topic Bioavailable phosphorus
calyptra
hairs
phosphatase activity
polyphosphate granules
self-luminescent bioreporter
Biología y Biomedicina / Biología
description Conventional assays to measure phosphorus in freshwater systems are sometimes not sufficient to quantify the actual bioavailable P for aquatic biota since some inorganic or organic P species may not be detected by chemical methods, and their bioavailability can be affected by a range of environmental factors. This situation could lead regulatory agencies to be unable to detect imminent ecosystem-degrading phenomena such as cyanobacterial blooms. It could also be an obstacle in studying the ecophysiological requirements of freshwater communities. P bioavailability in five rivers located in central Spain was analysed by a polyphasic approach (combinations of different marker types) based on cyanobacteria. This approach included a parallel study with the use of a self-luminescent P-cyanobacterial bioreporter based on a phosphatase alkaline promoter, determination of in situ alkaline phosphatase activities from cyanobacteria found at sampling sites, and the characterisation of cyanobacterial morphological features related to P bioavailability (hairs, polyphosphate granules and calyptras). An inverse relationship was found between values of bioavailable P, measured by the bioreporter and phosphatase activities. Cyanobacteria from sampling sites with low bioavailable P showed high phosphatase activity and vice versa, although some differences in values of this activity were observed in different cyanobacteria found at the same place, in relation to different growth strategies. Morphological characteristics associated with P limitation or P enrichment also varied between sampling locations. Cyanobacteria collected from sampling sites with reduced P bioavailability, measured by bioreporter and phosphatase activity, had a lower abundance of polyphosphate granules; those cyanobacteria capable of developing hairs or calyptras showed a greater abundance of these structures. Conversely, polyphosphate granules in cyanobacteria increased as P bioavailability increased as measured by the bioreporter and phosphatase activity. The study shows that the results of genetic, physiological and microscopic analyses based on these methods complement each other, implying that combining their findings would provide a more complete analysis of the nutrient status of running waters
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-03-15
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/717425
https://dx.doi.org/10.1016/j.scitotenv.2013.06.076
url http://hdl.handle.net/10486/717425
https://dx.doi.org/10.1016/j.scitotenv.2013.06.076
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
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repository.mail.fl_str_mv
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