The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis

Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of N...

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Autores: Pereira, Catia, Montalbán, Itziar A., Goicoa Mangado, Tomás, Ugarte Martínez, María Dolores, Correia, Sandra, Canhoto, Jorge M., Moncaleán, Paloma
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/30428
Acceso en línea:https://hdl.handle.net/2454/30428
Access Level:acceso abierto
Palabra clave:Embryogenic cell lines
Pinus halepensis
Somatic embryos
Water availability
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spelling The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesisPereira, CatiaMontalbán, Itziar A.Goicoa Mangado, TomásUgarte Martínez, María DoloresCorreia, SandraCanhoto, Jorge M.Moncaleán, PalomaEmbryogenic cell linesPinus halepensisSomatic embryosWater availabilityAim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability.MINECO, Spanish Government (project AGL2013-4700-C4-2R).Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)Estadística e Investigación OperativaEstatistika eta Ikerketa Operatiboa2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/30428reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MINECO//AGL2013-47000-R© 2017 INIA. This is an open access article distributed under the terms of the Creative Commons Attribution (CC-by) Spain 3.0 License.https://creativecommons.org/licenses/by/3.0/es/deed.es/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/304282026-06-17T12:41:47Z
dc.title.none.fl_str_mv The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
title The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
spellingShingle The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
Pereira, Catia
Embryogenic cell lines
Pinus halepensis
Somatic embryos
Water availability
title_short The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
title_full The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
title_fullStr The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
title_full_unstemmed The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
title_sort The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
dc.creator.none.fl_str_mv Pereira, Catia
Montalbán, Itziar A.
Goicoa Mangado, Tomás
Ugarte Martínez, María Dolores
Correia, Sandra
Canhoto, Jorge M.
Moncaleán, Paloma
author Pereira, Catia
author_facet Pereira, Catia
Montalbán, Itziar A.
Goicoa Mangado, Tomás
Ugarte Martínez, María Dolores
Correia, Sandra
Canhoto, Jorge M.
Moncaleán, Paloma
author_role author
author2 Montalbán, Itziar A.
Goicoa Mangado, Tomás
Ugarte Martínez, María Dolores
Correia, Sandra
Canhoto, Jorge M.
Moncaleán, Paloma
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Estadística e Investigación Operativa
Estatistika eta Ikerketa Operatiboa
dc.subject.none.fl_str_mv Embryogenic cell lines
Pinus halepensis
Somatic embryos
Water availability
topic Embryogenic cell lines
Pinus halepensis
Somatic embryos
Water availability
description Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/30428
url https://hdl.handle.net/2454/30428
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2013-47000-R
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/3.0/es/deed.es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/3.0/es/deed.es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)
publisher.none.fl_str_mv Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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