Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes

Almost all eukaryotes have transposable elements (TEs) against which they have developed defense mechanisms. In the Drosophila germline, the main transposable element (TE) regulation pathway is mediated by specific Piwi-interacting small RNAs (piRNAs). Nonetheless, for unknown reasons, TEs sometimes...

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Autores: Gámez Visairas, Víctor|||0000-0001-5234-4313, Romero-Soriano, Valèria|||0000-0003-1717-2928, Martí-Carreras, Joan|||0000-0002-0005-9761, Segarra-Carrillo, Eila, García Guerreiro, María Pilar|||0000-0001-9951-1879
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:252459
Acesso em linha:https://ddd.uab.cat/record/252459
https://dx.doi.org/urn:doi:10.3390/genes11020215
Access Level:Acceso aberto
Palavra-chave:Drosophila
Pirna pathway genes
Interspecific hybrids
Transposable elements
Deregulation
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spelling Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway GenesGámez Visairas, Víctor|||0000-0001-5234-4313Romero-Soriano, Valèria|||0000-0003-1717-2928Martí-Carreras, Joan|||0000-0002-0005-9761Segarra-Carrillo, EilaGarcía Guerreiro, María Pilar|||0000-0001-9951-1879DrosophilaPirna pathway genesInterspecific hybridsTransposable elementsDeregulationAlmost all eukaryotes have transposable elements (TEs) against which they have developed defense mechanisms. In the Drosophila germline, the main transposable element (TE) regulation pathway is mediated by specific Piwi-interacting small RNAs (piRNAs). Nonetheless, for unknown reasons, TEs sometimes escape cellular control during interspecific hybridization processes. Because the piRNA pathway genes are involved in piRNA biogenesis and TE control, we sequenced and characterized nine key genes from this pathway in Drosophila buzzatii and Drosophila koepferae species and studied their expression pattern in ovaries of both species and their F1 hybrids. We found that gene structure is, in general, maintained between both species and that two genes- armitage and aubergine- are under positive selection. Three genes- krimper, methyltransferase 2, and zucchini -displayed higher expression values in hybrids than both parental species, while others had RNA levels similar to the parental species with the highest expression. This suggests that the overexpression of some piRNA pathway genes can be a primary response to hybrid stress. Therefore, these results reinforce the hypothesis that TE deregulation may be due to the protein incompatibility caused by the rapid evolution of these genes, leading to a TE silencing failure, rather than to an underexpression of piRNA pathway genes. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/252459https://dx.doi.org/urn:doi:10.3390/genes11020215reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CGL2013-42432PMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CGL2017-89160PAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1346Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1379open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2524592026-06-06T12:50:31Z
dc.title.none.fl_str_mv Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
title Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
spellingShingle Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
Gámez Visairas, Víctor|||0000-0001-5234-4313
Drosophila
Pirna pathway genes
Interspecific hybrids
Transposable elements
Deregulation
title_short Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
title_full Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
title_fullStr Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
title_full_unstemmed Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
title_sort Drosophila Interspecific Hybridization Causes a Deregulation of the piRNA Pathway Genes
dc.creator.none.fl_str_mv Gámez Visairas, Víctor|||0000-0001-5234-4313
Romero-Soriano, Valèria|||0000-0003-1717-2928
Martí-Carreras, Joan|||0000-0002-0005-9761
Segarra-Carrillo, Eila
García Guerreiro, María Pilar|||0000-0001-9951-1879
author Gámez Visairas, Víctor|||0000-0001-5234-4313
author_facet Gámez Visairas, Víctor|||0000-0001-5234-4313
Romero-Soriano, Valèria|||0000-0003-1717-2928
Martí-Carreras, Joan|||0000-0002-0005-9761
Segarra-Carrillo, Eila
García Guerreiro, María Pilar|||0000-0001-9951-1879
author_role author
author2 Romero-Soriano, Valèria|||0000-0003-1717-2928
Martí-Carreras, Joan|||0000-0002-0005-9761
Segarra-Carrillo, Eila
García Guerreiro, María Pilar|||0000-0001-9951-1879
author2_role author
author
author
author
dc.subject.none.fl_str_mv Drosophila
Pirna pathway genes
Interspecific hybrids
Transposable elements
Deregulation
topic Drosophila
Pirna pathway genes
Interspecific hybrids
Transposable elements
Deregulation
description Almost all eukaryotes have transposable elements (TEs) against which they have developed defense mechanisms. In the Drosophila germline, the main transposable element (TE) regulation pathway is mediated by specific Piwi-interacting small RNAs (piRNAs). Nonetheless, for unknown reasons, TEs sometimes escape cellular control during interspecific hybridization processes. Because the piRNA pathway genes are involved in piRNA biogenesis and TE control, we sequenced and characterized nine key genes from this pathway in Drosophila buzzatii and Drosophila koepferae species and studied their expression pattern in ovaries of both species and their F1 hybrids. We found that gene structure is, in general, maintained between both species and that two genes- armitage and aubergine- are under positive selection. Three genes- krimper, methyltransferase 2, and zucchini -displayed higher expression values in hybrids than both parental species, while others had RNA levels similar to the parental species with the highest expression. This suggests that the overexpression of some piRNA pathway genes can be a primary response to hybrid stress. Therefore, these results reinforce the hypothesis that TE deregulation may be due to the protein incompatibility caused by the rapid evolution of these genes, leading to a TE silencing failure, rather than to an underexpression of piRNA pathway genes.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/252459
https://dx.doi.org/urn:doi:10.3390/genes11020215
url https://ddd.uab.cat/record/252459
https://dx.doi.org/urn:doi:10.3390/genes11020215
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CGL2013-42432P
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CGL2017-89160P
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1346
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1379
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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