The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity

H2A.Z variant has emerged as a critical player in regulating plant responses to environment; however, the mechanism by which H2A.Z mediates this regulation remains unclear. In Arabidopsis, H2A.Z has been proposed to have opposite effects on transcription depending on its localization within the gene...

Descripción completa

Detalles Bibliográficos
Autores: Gómez-Zambrano, Ángeles, Merini, Wiam, Calonje, Myriam
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/190588
Acceso en línea:http://hdl.handle.net/10261/190588
Access Level:acceso abierto
id ES_a635009c4d8bcbc73c2f6239a201fa9e
oai_identifier_str oai:digital.csic.es:10261/190588
network_acronym_str ES
network_name_str España
repository_id_str
spelling The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activityGómez-Zambrano, ÁngelesMerini, WiamCalonje, MyriamH2A.Z variant has emerged as a critical player in regulating plant responses to environment; however, the mechanism by which H2A.Z mediates this regulation remains unclear. In Arabidopsis, H2A.Z has been proposed to have opposite effects on transcription depending on its localization within the gene. These opposite roles have been assigned by correlating gene expression and H2A.Z enrichment analyses but without considering the impact of possible H2A.Z post-translational modifications. Here, we show that H2A.Z can be monoubiquitinated by the PRC1 components AtBMI1A/B/C. The incorporation of this modification is required for H2A.Z-mediated transcriptional repression through a mechanism that does not require PRC2 activity. Our data suggest that the dual role of H2A.Z in regulating gene expression depends on the modification that it carries, while the levels of H2A.Z within genes depend on the transcriptional activity.Peer ReviewedNature Publishing GroupConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/190588reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1905882026-05-22T06:33:51Z
dc.title.none.fl_str_mv The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
title The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
spellingShingle The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
Gómez-Zambrano, Ángeles
title_short The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
title_full The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
title_fullStr The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
title_full_unstemmed The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
title_sort The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity
dc.creator.none.fl_str_mv Gómez-Zambrano, Ángeles
Merini, Wiam
Calonje, Myriam
author Gómez-Zambrano, Ángeles
author_facet Gómez-Zambrano, Ángeles
Merini, Wiam
Calonje, Myriam
author_role author
author2 Merini, Wiam
Calonje, Myriam
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description H2A.Z variant has emerged as a critical player in regulating plant responses to environment; however, the mechanism by which H2A.Z mediates this regulation remains unclear. In Arabidopsis, H2A.Z has been proposed to have opposite effects on transcription depending on its localization within the gene. These opposite roles have been assigned by correlating gene expression and H2A.Z enrichment analyses but without considering the impact of possible H2A.Z post-translational modifications. Here, we show that H2A.Z can be monoubiquitinated by the PRC1 components AtBMI1A/B/C. The incorporation of this modification is required for H2A.Z-mediated transcriptional repression through a mechanism that does not require PRC2 activity. Our data suggest that the dual role of H2A.Z in regulating gene expression depends on the modification that it carries, while the levels of H2A.Z within genes depend on the transcriptional activity.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/190588
url http://hdl.handle.net/10261/190588
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415679035179008
score 15,81155

Similares en LA Referencia