Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.

Ruiz-Llorente, Lidia; Ruiz-Rodríguez, María Jesús; Savini, Claudia; González-Muñoz, Teresa; Riveiro-Falkenbach, Erica; Rodríguez-Peralto, José L; Bernabeu, Carmelo

Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.

Endoglin (CD105) is an auxiliary receptor of transforming growth factor (TGF)-β family members that is expressed in human melanomas. It is heterogeneously expressed by primary and metastatic melanoma cells, and endoglin targeting as a therapeutic strategy for melanoma tumors is currently been explor...

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Autores:	Ruiz-Llorente, Lidia, Ruiz-Rodríguez, María Jesús, Savini, Claudia, González-Muñoz, Teresa, Riveiro-Falkenbach, Erica, Rodríguez-Peralto, José L, Bernabeu, Carmelo
Tipo de recurso:	artículo
Fecha de publicación:	2023
País:	España
Institución:	Instituto de Salud Carlos III (ISCIII)
Repositorio:	Repisalud
Idioma:	inglés
OAI Identifier:	oai:repisalud.isciii.es:20.500.12105/17206
Acceso en línea:	http://hdl.handle.net/20.500.12105/17206
Access Level:	acceso abierto
Palabra clave:	Melanoma MicroRNAs Extracellular Vesicles Humans Endoglin Endothelial Cells Transforming Growth Factor beta

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spelling	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.Ruiz-Llorente, LidiaRuiz-Rodríguez, María JesúsSavini, ClaudiaGonzález-Muñoz, TeresaRiveiro-Falkenbach, EricaRodríguez-Peralto, José LBernabeu, CarmeloMelanomaMicroRNAsExtracellular VesiclesHumansEndoglinEndothelial CellsTransforming Growth Factor betaEndoglin (CD105) is an auxiliary receptor of transforming growth factor (TGF)-β family members that is expressed in human melanomas. It is heterogeneously expressed by primary and metastatic melanoma cells, and endoglin targeting as a therapeutic strategy for melanoma tumors is currently been explored. However, its involvement in tumor development and malignancy is not fully understood. Here, we find that endoglin expression correlates with malignancy of primary melanomas and cultured melanoma cell lines. Next, we have analyzed the effect of ectopic endoglin expression on two miRNAs (hsa-mir-214 and hsa-mir-370), both involved in melanoma tumor progression and endoglin regulation. We show that compared with control cells, overexpression of endoglin in the WM-164 melanoma cell line induces; (i) a significant increase of hsa-mir-214 levels in small extracellular vesicles (EVs) as well as an increased trend in cells; and (ii) significantly lower levels of hsa-mir-370 in the EVs fractions, whereas no significant differences were found in cells. As hsa-mir-214 and hsa-mir-370 are not just involved in melanoma tumor progression, but they can also target endoglin-expressing endothelial cells in the tumor vasculature, these results suggest a complex and differential regulatory mechanism involving the intracellular and extracellular signaling of hsa-mir-214 and hsa-mir-370 in melanoma development and progression.SpringerMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas (España)Centro de Investigación Biomédica en Red - CIBERER (Enfermedades Raras)Instituto de Salud Carlos III20242024-01-1720232023-04-2520232023-04-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/17206reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/172062026-06-12T12:43:37Z
dc.title.none.fl_str_mv	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
title	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
spellingShingle	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles. Ruiz-Llorente, Lidia Melanoma MicroRNAs Extracellular Vesicles Humans Endoglin Endothelial Cells Transforming Growth Factor beta
title_short	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
title_full	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
title_fullStr	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
title_full_unstemmed	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
title_sort	Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.
dc.creator.none.fl_str_mv	Ruiz-Llorente, Lidia Ruiz-Rodríguez, María Jesús Savini, Claudia González-Muñoz, Teresa Riveiro-Falkenbach, Erica Rodríguez-Peralto, José L Bernabeu, Carmelo
author	Ruiz-Llorente, Lidia
author_facet	Ruiz-Llorente, Lidia Ruiz-Rodríguez, María Jesús Savini, Claudia González-Muñoz, Teresa Riveiro-Falkenbach, Erica Rodríguez-Peralto, José L Bernabeu, Carmelo
author_role	author
author2	Ruiz-Rodríguez, María Jesús Savini, Claudia González-Muñoz, Teresa Riveiro-Falkenbach, Erica Rodríguez-Peralto, José L Bernabeu, Carmelo
author2_role	author author author author author author
dc.contributor.none.fl_str_mv	Ministerio de Ciencia, Innovación y Universidades (España) Consejo Superior de Investigaciones Científicas (España) Centro de Investigación Biomédica en Red - CIBERER (Enfermedades Raras) Instituto de Salud Carlos III
dc.subject.none.fl_str_mv	Melanoma MicroRNAs Extracellular Vesicles Humans Endoglin Endothelial Cells Transforming Growth Factor beta
topic	Melanoma MicroRNAs Extracellular Vesicles Humans Endoglin Endothelial Cells Transforming Growth Factor beta
description	Endoglin (CD105) is an auxiliary receptor of transforming growth factor (TGF)-β family members that is expressed in human melanomas. It is heterogeneously expressed by primary and metastatic melanoma cells, and endoglin targeting as a therapeutic strategy for melanoma tumors is currently been explored. However, its involvement in tumor development and malignancy is not fully understood. Here, we find that endoglin expression correlates with malignancy of primary melanomas and cultured melanoma cell lines. Next, we have analyzed the effect of ectopic endoglin expression on two miRNAs (hsa-mir-214 and hsa-mir-370), both involved in melanoma tumor progression and endoglin regulation. We show that compared with control cells, overexpression of endoglin in the WM-164 melanoma cell line induces; (i) a significant increase of hsa-mir-214 levels in small extracellular vesicles (EVs) as well as an increased trend in cells; and (ii) significantly lower levels of hsa-mir-370 in the EVs fractions, whereas no significant differences were found in cells. As hsa-mir-214 and hsa-mir-370 are not just involved in melanoma tumor progression, but they can also target endoglin-expressing endothelial cells in the tumor vasculature, these results suggest a complex and differential regulatory mechanism involving the intracellular and extracellular signaling of hsa-mir-214 and hsa-mir-370 in melanoma development and progression.
publishDate	2023
dc.date.none.fl_str_mv	2023 2023-04-25 2023 2023-04-25 2024 2024-01-17
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12105/17206
url	http://hdl.handle.net/20.500.12105/17206
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Springer
publisher.none.fl_str_mv	Springer
dc.source.none.fl_str_mv	reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII)
instname_str	Instituto de Salud Carlos III (ISCIII)
reponame_str	Repisalud
collection	Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles.

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