Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains

Campylobacter is the leading cause of bacterial diarrheal disease worldwide. Although most episodes of campylobacteriosis are self-limiting, antibiotic treatment is usually needed in patients with serious enteritis, and especially in childrens or the elderly. In the last years, antibiotic resistance...

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Autores: Silván, José Manuel, Zorraquín-Peña, Irene, González de Llano, Dolores, Moreno-Arribas, M. Victoria, Martínez-Rodríguez, Adolfo J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/192107
Acceso en línea:http://hdl.handle.net/10261/192107
Access Level:acceso abierto
Palabra clave:Antibacterial activity
Campylobacter
Silver nanoparticles
Antibiotic resistance
Multidrug resistant strains
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spelling Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strainsSilván, José ManuelZorraquín-Peña, IreneGonzález de Llano, DoloresMoreno-Arribas, M. VictoriaMartínez-Rodríguez, Adolfo J.Antibacterial activityCampylobacterSilver nanoparticlesAntibiotic resistanceMultidrug resistant strainsCampylobacter is the leading cause of bacterial diarrheal disease worldwide. Although most episodes of campylobacteriosis are self-limiting, antibiotic treatment is usually needed in patients with serious enteritis, and especially in childrens or the elderly. In the last years, antibiotic resistance in Campylobacter has become a major public health concern and a great interest exists in developing new antimicrobial strategies for reducing the impact of this food-borne pathogen on human health. Among them, the use of silver nanoparticles as antibacterial agents has taken on increased importance in the field of medicine. The aim of the present study was to evaluate the antimicrobial effectiveness of glutathione-stabilized silver nanoparticles (GSH-Ag NPs) against multidrug resistant (MDR) Campylobacter strains isolated from the chicken food chain (FC) and clinical patients (C). The results obtained showed that GSH-Ag NPs were highly effective against all MDR Campylobacter strains tested. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were in a range from 4.92 to 39.4 μg/mL and 9.85 to 39.4 μg/mL, respectively. Cytotoxicity assays were also assessed using human intestinal HT-29, Caco-2, and CCD-18 epithelial cells. Exposure of GSH-Ag NPs to intestinal cells showed a dose-dependent cytotoxic effect in all cell lines between 9.85 and 39.4 μg/mL. More than 60% of the tested Campylobacter strains were susceptible to GSH-Ag NPs concentrations ≤ 9.85 μg/mL, suggesting that practical inhibitory levels could be reached at low GSH-Ag NPs concentrations. Further works are needed with the purpose to evaluate the practical implications of the toxicity studies and to know more about other attributes linked to the biological compatibility. This behavior makes GSH-Ag NPs as a promising tool for the design of novel antibacterial agents for controlling Campylobacter.This work was founded through Project AGL2013-47694-R and AGL2015-64522-C2-R from the Consejo Superior de Investigaciones Científicas (Spain).Peer ReviewedFrontiers MediaConsejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920182019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versionPublisher's versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/192107reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47694-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64522-C2-Rhttps://doi.org/10.3389/fmicb.2018.00458Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1921072026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
title Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
spellingShingle Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
Silván, José Manuel
Antibacterial activity
Campylobacter
Silver nanoparticles
Antibiotic resistance
Multidrug resistant strains
title_short Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
title_full Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
title_fullStr Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
title_full_unstemmed Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
title_sort Antibacterial activity of glutathione-stabilized silver nanoparticles against Campylobacter multidrug-resistant strains
dc.creator.none.fl_str_mv Silván, José Manuel
Zorraquín-Peña, Irene
González de Llano, Dolores
Moreno-Arribas, M. Victoria
Martínez-Rodríguez, Adolfo J.
author Silván, José Manuel
author_facet Silván, José Manuel
Zorraquín-Peña, Irene
González de Llano, Dolores
Moreno-Arribas, M. Victoria
Martínez-Rodríguez, Adolfo J.
author_role author
author2 Zorraquín-Peña, Irene
González de Llano, Dolores
Moreno-Arribas, M. Victoria
Martínez-Rodríguez, Adolfo J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antibacterial activity
Campylobacter
Silver nanoparticles
Antibiotic resistance
Multidrug resistant strains
topic Antibacterial activity
Campylobacter
Silver nanoparticles
Antibiotic resistance
Multidrug resistant strains
description Campylobacter is the leading cause of bacterial diarrheal disease worldwide. Although most episodes of campylobacteriosis are self-limiting, antibiotic treatment is usually needed in patients with serious enteritis, and especially in childrens or the elderly. In the last years, antibiotic resistance in Campylobacter has become a major public health concern and a great interest exists in developing new antimicrobial strategies for reducing the impact of this food-borne pathogen on human health. Among them, the use of silver nanoparticles as antibacterial agents has taken on increased importance in the field of medicine. The aim of the present study was to evaluate the antimicrobial effectiveness of glutathione-stabilized silver nanoparticles (GSH-Ag NPs) against multidrug resistant (MDR) Campylobacter strains isolated from the chicken food chain (FC) and clinical patients (C). The results obtained showed that GSH-Ag NPs were highly effective against all MDR Campylobacter strains tested. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were in a range from 4.92 to 39.4 μg/mL and 9.85 to 39.4 μg/mL, respectively. Cytotoxicity assays were also assessed using human intestinal HT-29, Caco-2, and CCD-18 epithelial cells. Exposure of GSH-Ag NPs to intestinal cells showed a dose-dependent cytotoxic effect in all cell lines between 9.85 and 39.4 μg/mL. More than 60% of the tested Campylobacter strains were susceptible to GSH-Ag NPs concentrations ≤ 9.85 μg/mL, suggesting that practical inhibitory levels could be reached at low GSH-Ag NPs concentrations. Further works are needed with the purpose to evaluate the practical implications of the toxicity studies and to know more about other attributes linked to the biological compatibility. This behavior makes GSH-Ag NPs as a promising tool for the design of novel antibacterial agents for controlling Campylobacter.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
Publisher's version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/192107
url http://hdl.handle.net/10261/192107
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47694-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64522-C2-R
https://doi.org/10.3389/fmicb.2018.00458
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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