Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides

10 páginas, 6 figuras, 2 tablas

Detalles Bibliográficos
Autores: Gestal, C., Costa, M. M., Figueras Huerta, Antonio, Novoa, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/50398
Acceso en línea:http://hdl.handle.net/10261/50398
Access Level:acceso abierto
Palabra clave:Antimicrobial peptides
Clam
Immune response
Myticin
Mytilin
Ruditapes decussatus
SSH
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spelling Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptidesGestal, C.Costa, M. M.Figueras Huerta, AntonioNovoa, BeatrizAntimicrobial peptidesClamImmune responseMyticinMytilinRuditapes decussatusSSH10 páginas, 6 figuras, 2 tablasSuppression subtractive hybridization was used to identify differentiallyexpressedgenes in hemocytes from carpet-shell clam Ruditapes decussatus stimulated with a mixture of dead bacterial strains. Putative function could be assigned to 100 of the 253 sequenced cDNAs. Based on sequence homologies, 3.16% of the total identified genes were possibly related to immune functions. Clam myticin isoforms 1, 2 and 3, and clam mytilin, with similarity with myticins and mytilins previously reported on Mytilus galloprovincialis were identified and characterized for the first time in clams. The analysis of their expression levels by quantitative PCR showed that they were induced by bacterial challenge. The results obtained in this work could be the first step leading to the understanding of molecular mechanisms by which these economically important marine bivalves respond to pathogensThis work has been partially funded by the European Commission through the Marie Curie European Reintegration Project ERG-CT-2005-518007 and the Integrated Research project FOOD-CT-2005-007103, by the Xunta de Galicia (project PGIDIT06PXIC402124A4) and by the Spanish MEC (AGL2003-02454). We thank Philippe Roch for providing the mix of dead bacteria. C.G. wishes to acknowledge additional funding from the Spanish Ministerio de Educación y Ciencia through the “Ramón y Cajal” Contract, and M.M.C. through the FPI Spanish research grant.Peer reviewedElsevier201220122007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/50398reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.gene.2007.07.030info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/503982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
title Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
spellingShingle Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
Gestal, C.
Antimicrobial peptides
Clam
Immune response
Myticin
Mytilin
Ruditapes decussatus
SSH
title_short Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
title_full Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
title_fullStr Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
title_full_unstemmed Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
title_sort Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: Identification of new antimicrobial peptides
dc.creator.none.fl_str_mv Gestal, C.
Costa, M. M.
Figueras Huerta, Antonio
Novoa, Beatriz
author Gestal, C.
author_facet Gestal, C.
Costa, M. M.
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Costa, M. M.
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Antimicrobial peptides
Clam
Immune response
Myticin
Mytilin
Ruditapes decussatus
SSH
topic Antimicrobial peptides
Clam
Immune response
Myticin
Mytilin
Ruditapes decussatus
SSH
description 10 páginas, 6 figuras, 2 tablas
publishDate 2007
dc.date.none.fl_str_mv 2007
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/50398
url http://hdl.handle.net/10261/50398
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.gene.2007.07.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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