Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus

Understanding the mechanisms involved in the response to abiotic stress is essential to enhance plant stress resilience and ensure sustainable agriculture. In this study we report different roles for two closely related MYB transcription factors (TFs), MYB13 and MYB15, in the legume model Lotus japo...

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Autores: Monje Rueda, María Dolores, Pal'ove-Balang, Peter, Márquez Cabeza, Antonio José, Betti, Marco, García Calderón, Margarita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/176198
Acceso en línea:https://hdl.handle.net/11441/176198
https://doi.org/10.1016/j.stress.2025.100913
Access Level:acceso abierto
Palabra clave:Abiotic stress
UV-B irradiation and salt
Isoflavonoids
Transcription factors
Lotus japonicus
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spelling Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicusMonje Rueda, María DoloresPal'ove-Balang, PeterMárquez Cabeza, Antonio JoséBetti, MarcoGarcía Calderón, MargaritaAbiotic stressUV-B irradiation and saltIsoflavonoidsTranscription factorsLotus japonicusUnderstanding the mechanisms involved in the response to abiotic stress is essential to enhance plant stress resilience and ensure sustainable agriculture. In this study we report different roles for two closely related MYB transcription factors (TFs), MYB13 and MYB15, in the legume model Lotus japonicus. Different growth and biochemical parameters were determined for mutant lines impaired in these TFs under either control conditions or under two different types of abiotic stress: salinity and UV-B irradiation. Our findings show that MYB15 positively regulates isoflavonoid biosynthesis under UV-B irradiation but does not affect salt tolerance. In contrast, MYB13 does not appear to be involved in the UV-B response but plays a significant role in salt stress tolerance, as specific mutants lacking MYB13 exhibited increased NaCl resistance. Further analysis revealed that salt tolerance in Ljmyb13 mutants is associated with changes in vestitol and chloride ion (Cl-) levels, as well as modifications in root architecture. Moreover, the growth of both mutants was enhanced in the absence of abiotic stress, but only under non-symbiotic conditions. These findings highlight the differential roles of MYB13 and MYB15 in regulating stress responses and provide insights into potential mechanisms of salt stress adaptation in L. japonicus.ElsevierBioquímica Vegetal y Biología MolecularAGR286: Biotecnología-QuímicaMinisterio de Ciencia, Innovación y Universidades (MICIU). EspañaAgencia Estatal de Investigación. EspañaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/176198https://doi.org/10.1016/j.stress.2025.100913reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlant Stress, 17, 100913.PID2021–122353OB-I00RTI-2018–093571-B100PRE2019–088920https://www.sciencedirect.com/science/article/pii/S2667064X25001812info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1761982026-06-17T12:51:07Z
dc.title.none.fl_str_mv Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
title Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
spellingShingle Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
Monje Rueda, María Dolores
Abiotic stress
UV-B irradiation and salt
Isoflavonoids
Transcription factors
Lotus japonicus
title_short Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
title_full Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
title_fullStr Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
title_full_unstemmed Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
title_sort Differential involvement of MYB13 and MYB15 transcription factors in isoflavonoid biosynthesis and tolerance to salt stress in Lotus japonicus
dc.creator.none.fl_str_mv Monje Rueda, María Dolores
Pal'ove-Balang, Peter
Márquez Cabeza, Antonio José
Betti, Marco
García Calderón, Margarita
author Monje Rueda, María Dolores
author_facet Monje Rueda, María Dolores
Pal'ove-Balang, Peter
Márquez Cabeza, Antonio José
Betti, Marco
García Calderón, Margarita
author_role author
author2 Pal'ove-Balang, Peter
Márquez Cabeza, Antonio José
Betti, Marco
García Calderón, Margarita
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
AGR286: Biotecnología-Química
Ministerio de Ciencia, Innovación y Universidades (MICIU). España
Agencia Estatal de Investigación. España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
dc.subject.none.fl_str_mv Abiotic stress
UV-B irradiation and salt
Isoflavonoids
Transcription factors
Lotus japonicus
topic Abiotic stress
UV-B irradiation and salt
Isoflavonoids
Transcription factors
Lotus japonicus
description Understanding the mechanisms involved in the response to abiotic stress is essential to enhance plant stress resilience and ensure sustainable agriculture. In this study we report different roles for two closely related MYB transcription factors (TFs), MYB13 and MYB15, in the legume model Lotus japonicus. Different growth and biochemical parameters were determined for mutant lines impaired in these TFs under either control conditions or under two different types of abiotic stress: salinity and UV-B irradiation. Our findings show that MYB15 positively regulates isoflavonoid biosynthesis under UV-B irradiation but does not affect salt tolerance. In contrast, MYB13 does not appear to be involved in the UV-B response but plays a significant role in salt stress tolerance, as specific mutants lacking MYB13 exhibited increased NaCl resistance. Further analysis revealed that salt tolerance in Ljmyb13 mutants is associated with changes in vestitol and chloride ion (Cl-) levels, as well as modifications in root architecture. Moreover, the growth of both mutants was enhanced in the absence of abiotic stress, but only under non-symbiotic conditions. These findings highlight the differential roles of MYB13 and MYB15 in regulating stress responses and provide insights into potential mechanisms of salt stress adaptation in L. japonicus.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/176198
https://doi.org/10.1016/j.stress.2025.100913
url https://hdl.handle.net/11441/176198
https://doi.org/10.1016/j.stress.2025.100913
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plant Stress, 17, 100913.
PID2021–122353OB-I00
RTI-2018–093571-B100
PRE2019–088920
https://www.sciencedirect.com/science/article/pii/S2667064X25001812
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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