Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina

The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two sub...

Full description

Bibliographic Details
Authors: Galisteo Gómez, Cristina, Sánchez-Porro Álvarez, Cristina, Ruiz de la Haba, Rafael, López Hermoso, Clara, Fernández González, Ana Beatriz, Farias, María Eugenia, Ventosa Ucero, Antonio
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/88720
Online Access:https://hdl.handle.net/11441/88720
https://doi.org/10.3390/microorganisms7080241
Access Level:Open access
Keyword:Salinivibrio
Salinivibrio socompensis
bacterial taxonomy
halophilic bacteria
new species
hypersaline lake
id ES_a5b920d00eb987fc34eadc0d7fed22f5
oai_identifier_str oai:idus.us.es:11441/88720
network_acronym_str ES
network_name_str España
repository_id_str
spelling Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, ArgentinaGalisteo Gómez, CristinaSánchez-Porro Álvarez, CristinaRuiz de la Haba, RafaelLópez Hermoso, ClaraFernández González, Ana BeatrizFarias, María EugeniaVentosa Ucero, AntonioSalinivibrioSalinivibrio socompensisbacterial taxonomyhalophilic bacterianew specieshypersaline lakeThe genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T , was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T , including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA–DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%–20% (w/v) NaCl, pH 6–10, and 10–42 ◦C, with optimum growth at 7.0%–7.5% (w/v) NaCl, pH 8.0, and 37 ◦C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T )Ministerio de Economía y Competitividad de España (MINECO)-CGL2017-83385-PMinisterio de Ciencia y Tecnología de Argentina-PICT1707MDPIMicrobiología y Parasitología2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/88720https://doi.org/10.3390/microorganisms7080241reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicroorganisms, 7 (8), 241.CGL2017-83385-PPICT1707https://doi.org/10.3390/microorganisms7080241info:eu-repo/semantics/openAccessoai:idus.us.es:11441/887202026-06-17T12:51:07Z
dc.title.none.fl_str_mv Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
title Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
spellingShingle Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
Galisteo Gómez, Cristina
Salinivibrio
Salinivibrio socompensis
bacterial taxonomy
halophilic bacteria
new species
hypersaline lake
title_short Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
title_full Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
title_fullStr Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
title_full_unstemmed Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
title_sort Characterization of Salinivibrio socompensis sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
dc.creator.none.fl_str_mv Galisteo Gómez, Cristina
Sánchez-Porro Álvarez, Cristina
Ruiz de la Haba, Rafael
López Hermoso, Clara
Fernández González, Ana Beatriz
Farias, María Eugenia
Ventosa Ucero, Antonio
author Galisteo Gómez, Cristina
author_facet Galisteo Gómez, Cristina
Sánchez-Porro Álvarez, Cristina
Ruiz de la Haba, Rafael
López Hermoso, Clara
Fernández González, Ana Beatriz
Farias, María Eugenia
Ventosa Ucero, Antonio
author_role author
author2 Sánchez-Porro Álvarez, Cristina
Ruiz de la Haba, Rafael
López Hermoso, Clara
Fernández González, Ana Beatriz
Farias, María Eugenia
Ventosa Ucero, Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
dc.subject.none.fl_str_mv Salinivibrio
Salinivibrio socompensis
bacterial taxonomy
halophilic bacteria
new species
hypersaline lake
topic Salinivibrio
Salinivibrio socompensis
bacterial taxonomy
halophilic bacteria
new species
hypersaline lake
description The genus Salinivibrio belongs to the family Vibrionaceae and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35T , was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35T , including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and in silico DNA–DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%–20% (w/v) NaCl, pH 6–10, and 10–42 ◦C, with optimum growth at 7.0%–7.5% (w/v) NaCl, pH 8.0, and 37 ◦C, respectively. Strain S35T was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C16:0, C16:1 ω7c and C16:1 ω6c, and C18:1 ω7c and/or C18:1 ω6c. ANI, GGDC, and core-genome analysis determined that strain S35T constitutes a novel species of the genus Salinivibrio, for which the name Salinivibrio socompensis sp. nov. is proposed. The type strain is S35T (= CECT 9634T = BNM 0535T )
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/88720
https://doi.org/10.3390/microorganisms7080241
url https://hdl.handle.net/11441/88720
https://doi.org/10.3390/microorganisms7080241
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microorganisms, 7 (8), 241.
CGL2017-83385-P
PICT1707
https://doi.org/10.3390/microorganisms7080241
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415639083384832
score 15.301603

Similar Items