The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection

Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inh...

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Autores: Bleriot, Ines, Blasco, Lucia, Pacios, Olga, Fernández-García, Laura, Ambroa, Antón, López, María, Ortiz-Cartagena, Concha, Cuenca, Felipe Fernández, Oteo-Iglesias, Jesus, Pascual, Álvaro, Martínez-Martínez, Luis, Domingo-Calap, Pilar, Wood, Thomas K, Tomás, María
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/14469
Acceso en línea:http://hdl.handle.net/20.500.12105/14469
Access Level:acceso abierto
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spelling The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infectionBleriot, InesBlasco, LuciaPacios, OlgaFernández-García, LauraAmbroa, AntónLópez, MaríaOrtiz-Cartagena, ConchaCuenca, Felipe FernándezOteo-Iglesias, JesusPascual, ÁlvaroMartínez-Martínez, LuisDomingo-Calap, PilarWood, Thomas KTomás, MaríaSince their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inhibition by its intrinsic expression in clinical strains of Klebsiella pneumoniae carrying the lncL plasmid, which harbours the carbapenemase OXA-48 and the PemK/PemI TA system. Furthermore, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneumoniae ATCC10031 was also studied. The results showed that induced expression of the whole TA system did not inhibit phage infection, whereas overexpression of the pemK toxin prevented early infection. To investigate the molecular mechanism involved in the PemK toxin-mediated inhibition of phage infection, assays measuring metabolic activity and viability were performed, revealing that overexpression of the PemK toxin led to dormancy of the bacteria. Thus, we demonstrate that the PemK/PemI TA system plays a role in phage infection and that the action of the free toxin induces a dormant state in the cells, resulting in inhibition of phage infections.Nature Publishing GroupInstituto de Salud Carlos IIIPlan Nacional de I+D+i (España)Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)Sociedad Española de Enfermedades Infecciosas y Microbiología ClínicaXunta de Galicia (España)20222022-05-2420222022-03-1620222022-03-16research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12105/14469reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/144692026-06-12T12:43:37Z
dc.title.none.fl_str_mv The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
title The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
spellingShingle The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
Bleriot, Ines
title_short The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
title_full The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
title_fullStr The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
title_full_unstemmed The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
title_sort The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
dc.creator.none.fl_str_mv Bleriot, Ines
Blasco, Lucia
Pacios, Olga
Fernández-García, Laura
Ambroa, Antón
López, María
Ortiz-Cartagena, Concha
Cuenca, Felipe Fernández
Oteo-Iglesias, Jesus
Pascual, Álvaro
Martínez-Martínez, Luis
Domingo-Calap, Pilar
Wood, Thomas K
Tomás, María
author Bleriot, Ines
author_facet Bleriot, Ines
Blasco, Lucia
Pacios, Olga
Fernández-García, Laura
Ambroa, Antón
López, María
Ortiz-Cartagena, Concha
Cuenca, Felipe Fernández
Oteo-Iglesias, Jesus
Pascual, Álvaro
Martínez-Martínez, Luis
Domingo-Calap, Pilar
Wood, Thomas K
Tomás, María
author_role author
author2 Blasco, Lucia
Pacios, Olga
Fernández-García, Laura
Ambroa, Antón
López, María
Ortiz-Cartagena, Concha
Cuenca, Felipe Fernández
Oteo-Iglesias, Jesus
Pascual, Álvaro
Martínez-Martínez, Luis
Domingo-Calap, Pilar
Wood, Thomas K
Tomás, María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Plan Nacional de I+D+i (España)
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
Xunta de Galicia (España)
description Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inhibition by its intrinsic expression in clinical strains of Klebsiella pneumoniae carrying the lncL plasmid, which harbours the carbapenemase OXA-48 and the PemK/PemI TA system. Furthermore, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneumoniae ATCC10031 was also studied. The results showed that induced expression of the whole TA system did not inhibit phage infection, whereas overexpression of the pemK toxin prevented early infection. To investigate the molecular mechanism involved in the PemK toxin-mediated inhibition of phage infection, assays measuring metabolic activity and viability were performed, revealing that overexpression of the PemK toxin led to dormancy of the bacteria. Thus, we demonstrate that the PemK/PemI TA system plays a role in phage infection and that the action of the free toxin induces a dormant state in the cells, resulting in inhibition of phage infections.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-24
2022
2022-03-16
2022
2022-03-16
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/14469
url http://hdl.handle.net/20.500.12105/14469
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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