Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein

10 páginas, 6 figuras, 1 tabla.-- et al.-- El pdf del artículo es la versión post-print.

Detalhes bibliográficos
Autores: Villar-Tajadura, M. Antonia, Pérez González, Pilar
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2010
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49605
Acesso em linha:http://hdl.handle.net/10261/49605
Access Level:acceso abierto
Palavra-chave:Cell wall yeast
G Proteins
Organisms/Yeast
Signal transduction
Fission yeast
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spelling Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating proteinVillar-Tajadura, M. AntoniaPérez González, PilarCell wall yeastG ProteinsOrganisms/YeastSignal transductionFission yeast10 páginas, 6 figuras, 1 tabla.-- et al.-- El pdf del artículo es la versión post-print.Rho GTPase-activating proteins (GAPs) are responsible for the inactivation of Rho GTPases, which are involved in the regulation of critical biological responses in eukaryotic cells, ranging from cell cycle control to cellular morphogenesis. The genome of fission yeast Schizosaccharomyces pombe contains six genes coding for putative Rho GTPases, whereas nine genes code for predicted Rho GAPs (Rga1 to Rga9). One of them, Rga4, has been recently described as a Cdc42 GAP, involved in the control of cell diameter and symmetry in fission yeast. In this work we show that Rga4 is also a Rho2 GAP that negatively modulates the activity of the cell integrity pathway and its main effector, MAPK Pmk1. The DYRK-type protein kinase Pom1, which regulates both the localization and phosphorylation state of Rga4, is also a negative regulator of the Pmk1 pathway, but this control is not dependent upon the Rga4 role as a Rho2-GAP. Hence, two subsets of Rga4 negatively regulate Cdc42 and Rho2 functions in a specific and unrelated way. Finally, we show that Rga7, another Rho2 GAP, down-regulates the Pmk1 pathway in addition to Rga4. These results reinforce the notion of the existence of complex mechanisms determining the selectivity of Rho GAPs toward Rho GTPases and their functions.This work was supported by Grants BFU2007-60675 from Ministerio de Cienicia e Innovación and GR231 from Junta de Castilla y León, Spain (to P. P.) and Grants BFU2008-01653 from Ministerio de Cienicia e Innovacón and 08725/PI/08 from Fundación Séneca (Región de Murcia), Spain (to J. C.).Peer reviewedAmerican Society for Biochemistry and Molecular Biology201220122010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/49605reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1074/jbc.M109.071027info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/496052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
title Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
spellingShingle Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
Villar-Tajadura, M. Antonia
Cell wall yeast
G Proteins
Organisms/Yeast
Signal transduction
Fission yeast
title_short Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
title_full Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
title_fullStr Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
title_full_unstemmed Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
title_sort Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
dc.creator.none.fl_str_mv Villar-Tajadura, M. Antonia
Pérez González, Pilar
author Villar-Tajadura, M. Antonia
author_facet Villar-Tajadura, M. Antonia
Pérez González, Pilar
author_role author
author2 Pérez González, Pilar
author2_role author
dc.subject.none.fl_str_mv Cell wall yeast
G Proteins
Organisms/Yeast
Signal transduction
Fission yeast
topic Cell wall yeast
G Proteins
Organisms/Yeast
Signal transduction
Fission yeast
description 10 páginas, 6 figuras, 1 tabla.-- et al.-- El pdf del artículo es la versión post-print.
publishDate 2010
dc.date.none.fl_str_mv 2010
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/49605
url http://hdl.handle.net/10261/49605
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1074/jbc.M109.071027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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