Cerebrospinal fluid-derived circulating tumour DNA better represents the genomic alterations of brain tumours than plasma

Cell-free circulating tumour DNA (ctDNA) in plasma has been shown to be informative of the genomic alterations present in tumours and has been used to monitor tumour progression and response to treatments. However, patients with brain tumours do not present with or present with low amounts of ctDNA...

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Detalhes bibliográficos
Autores: De Mattos-Arruda, Leticia|||0000-0002-4992-3645, Mayor, Regina, Ng, Charlotte K. Y., Weigelt, Britta|||0000-0001-9927-1270, Martinez-Ricarte, Fran|||0000-0003-4567-0828, Torrejon, Davis, Oliveira, Mafalda|||0000-0001-9152-8799, Arias, Alexandra, Raventós, Carolina, Tang, Jiabin, Guerini-Rocco, Elena, Martinez-Saez, Elena|||0000-0001-6004-5364, Lois, Sergio|||0000-0002-6397-8693, Marín, Oscar, de la Cruz, Xavier|||0000-0002-9738-8472, Piscuoglio, Salvatore|||0000-0003-2686-2939, Towers, Russel, Vivancos, Ana|||0000-0003-2888-6512, Peg, Vicente|||0000-0002-5203-6166, Ramón y Cajal, Santiago|||0000-0002-3867-1390, Carles, Joan|||0000-0002-9983-5934, Rodon, Jordi|||0000-0002-1032-9091, González-Cao, María|||0000-0003-3791-540X, Tabernero, Josep|||0000-0002-2495-8139, Felip, Enriqueta|||0000-0002-7620-0098, Sahuquillo Barris, Juan|||0000-0003-0713-5875, Berger, Michael F., Cortés Castán, Javier|||0000-0001-7623-1583, Reis-Filho, Jorge S.|||0000-0003-2969-3173, Seoane Suárez, Joan|||0000-0002-6541-5974
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:254481
Acesso em linha:https://ddd.uab.cat/record/254481
https://dx.doi.org/urn:doi:10.1038/ncomms9839
Access Level:acceso abierto
Descrição
Resumo:Cell-free circulating tumour DNA (ctDNA) in plasma has been shown to be informative of the genomic alterations present in tumours and has been used to monitor tumour progression and response to treatments. However, patients with brain tumours do not present with or present with low amounts of ctDNA in plasma precluding the genomic characterization of brain cancer through plasma ctDNA. Here we show that ctDNA derived from central nervous system tumours is more abundantly present in the cerebrospinal fluid (CSF) than in plasma. Massively parallel sequencing of CSF ctDNA more comprehensively characterizes the genomic alterations of brain tumours than plasma, allowing the identification of actionable brain tumour somatic mutations. We show that CSF ctDNA levels longitudinally fluctuate in time and follow the changes in brain tumour burden providing biomarkers to monitor brain malignancies. Moreover, CSF ctDNA is shown to facilitate and complement the diagnosis of leptomeningeal carcinomatosis. DNA circulating in the plasma of cancer patients carries features of the primary tumour, however such DNA is found in low levels in brain cancer patients. Here, the authors show that circulating tumour DNA can be detected in the cerebral spinal fluid of cancer patients and that this better recapitulates the primary tumour compared to DNA from the plasma.

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