A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots
Ethylene, nitric oxide (NO) and glutathione (GSH) increase in Fe-deficient roots of Strategy I species where they participate in the up-regulation of Fe acquisition genes. However, S-nitrosoglutathione (GSNO), derived from NO and GSH, decreases in Fe-deficient roots. GSNO content is regulated by the...
| Autores: | , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/54578 |
| Acesso em linha: | https://hdl.handle.net/10171/54578 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biología Vegetal y Animal, Ecología Ethylene Glutathione (GSH) Iron Long distance iron signal (LODIS) Nitric oxide (NO) Phloem S-nitrosoglutathione (GSNO) S-nitrosoglutathione reductase (GSNOR) |
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A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana rootsGarcía-del-Rosal, M.J. (María José)|||/items/631557c9-1e7d-4252-8870-87d2ad3b1726Corpas, F.J. (Francisco Javier)|||/items/84be5386-440c-474a-a0fd-f67267dfe7acLucena, C. (Carlos)|||/items/b121bd8d-e0c2-4d88-b2c3-0ee61b13eb91Alcántara, E. (Esteban)|||/items/929c81c3-fbff-409c-947b-b0116f5e2b16Pérez-Vicente, R. (Rafael)|||/items/13f2ffef-e5ea-402a-8d47-31a9001c9c38Zamarreño-Arregui, A.M. (Angel María)|||/items/316fe376-3b6c-4f68-b6da-03cf1fdab968Bacaicoa, E. (Eva)|||/items/bf776d80-fd33-4c82-ae62-b50ca79eb262García-Mina, J.M. (José María)|||/items/815053fc-2f39-4f00-aed0-c5f6ee370258Bauer, P. (Petra)|||/items/3b95111e-a8e4-445f-b124-fcacffa93067Romera, F.J. (Francisco J.)|||/items/f0a732ab-66b2-4621-b4c7-c96b3a5a65e1Biología Vegetal y Animal, EcologíaEthyleneGlutathione (GSH)IronLong distance iron signal (LODIS)Nitric oxide (NO)PhloemS-nitrosoglutathione (GSNO)S-nitrosoglutathione reductase (GSNOR)Ethylene, nitric oxide (NO) and glutathione (GSH) increase in Fe-deficient roots of Strategy I species where they participate in the up-regulation of Fe acquisition genes. However, S-nitrosoglutathione (GSNO), derived from NO and GSH, decreases in Fe-deficient roots. GSNO content is regulated by the GSNO-degrading enzyme S-nitrosoglutathione reductase (GSNOR). On the other hand, there are several results showing that the regulation of Fe acquisition genes does not solely depend on hormones and signaling molecules (such as ethylene or NO), which would act as activators, but also on the internal Fe content of plants, which would act as a repressor. Moreover, different results suggest that total Fe in roots is not the repressor of Fe acquisition genes, but rather the repressor is a Fe signal that moves from shoots to roots through the phloem [hereafter named LOng Distance Iron Signal (LODIS)]. To look further in the possible interactions between LODIS, ethylene and GSNOR, we compared Arabidopsis WT Columbia and LODIS-deficient mutant opt3-2 plants subjected to different Fe treatments that alter LODIS content. The opt3-2 mutant is impaired in the loading of shoot Fe into the phloem and presents constitutive expression of Fe acquisition genes. In roots of both Columbia and opt3-2 plants we determined 1-aminocyclopropane1-carboxylic acid (ACC, ethylene precursor), expression of ethylene synthesis and signaling genes, and GSNOR expression and activity. The results obtained showed that both 'ethylene' (ACC and the expression of ethylene synthesis and signaling genes) and 'GSNOR' (expression and activity) increased in Fe-deficient WT Columbia roots. Additionally, Fe-sufficient opt3-2 roots had higher 'ethylene' and 'GSNOR' than Fe-sufficient WT Columbia roots. The increase of both 'ethylene' and 'GSNOR' was not related to the total root Fe content but to the absence of a Fe shoot signal (LODIS), and was associated with the up-regulation of Fe acquisition genes. The possible relationship between GSNOR(GSNO) and ethylene is discussed.Dadun. Depósito Académico Digital Universidad de Navarra20182018-10-0820182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/54578reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/545782026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| title |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| spellingShingle |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots García-del-Rosal, M.J. (María José)|||/items/631557c9-1e7d-4252-8870-87d2ad3b1726 Biología Vegetal y Animal, Ecología Ethylene Glutathione (GSH) Iron Long distance iron signal (LODIS) Nitric oxide (NO) Phloem S-nitrosoglutathione (GSNO) S-nitrosoglutathione reductase (GSNOR) |
| title_short |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| title_full |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| title_fullStr |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| title_full_unstemmed |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| title_sort |
A shoot Fe signaling pathway requiring the OPT3 transporter controls GSNO reductase and ethylene in arabidopsis thaliana roots |
| dc.creator.none.fl_str_mv |
García-del-Rosal, M.J. (María José)|||/items/631557c9-1e7d-4252-8870-87d2ad3b1726 Corpas, F.J. (Francisco Javier)|||/items/84be5386-440c-474a-a0fd-f67267dfe7ac Lucena, C. (Carlos)|||/items/b121bd8d-e0c2-4d88-b2c3-0ee61b13eb91 Alcántara, E. (Esteban)|||/items/929c81c3-fbff-409c-947b-b0116f5e2b16 Pérez-Vicente, R. (Rafael)|||/items/13f2ffef-e5ea-402a-8d47-31a9001c9c38 Zamarreño-Arregui, A.M. (Angel María)|||/items/316fe376-3b6c-4f68-b6da-03cf1fdab968 Bacaicoa, E. (Eva)|||/items/bf776d80-fd33-4c82-ae62-b50ca79eb262 García-Mina, J.M. (José María)|||/items/815053fc-2f39-4f00-aed0-c5f6ee370258 Bauer, P. (Petra)|||/items/3b95111e-a8e4-445f-b124-fcacffa93067 Romera, F.J. (Francisco J.)|||/items/f0a732ab-66b2-4621-b4c7-c96b3a5a65e1 |
| author |
García-del-Rosal, M.J. (María José)|||/items/631557c9-1e7d-4252-8870-87d2ad3b1726 |
| author_facet |
García-del-Rosal, M.J. (María José)|||/items/631557c9-1e7d-4252-8870-87d2ad3b1726 Corpas, F.J. (Francisco Javier)|||/items/84be5386-440c-474a-a0fd-f67267dfe7ac Lucena, C. (Carlos)|||/items/b121bd8d-e0c2-4d88-b2c3-0ee61b13eb91 Alcántara, E. (Esteban)|||/items/929c81c3-fbff-409c-947b-b0116f5e2b16 Pérez-Vicente, R. (Rafael)|||/items/13f2ffef-e5ea-402a-8d47-31a9001c9c38 Zamarreño-Arregui, A.M. (Angel María)|||/items/316fe376-3b6c-4f68-b6da-03cf1fdab968 Bacaicoa, E. (Eva)|||/items/bf776d80-fd33-4c82-ae62-b50ca79eb262 García-Mina, J.M. (José María)|||/items/815053fc-2f39-4f00-aed0-c5f6ee370258 Bauer, P. (Petra)|||/items/3b95111e-a8e4-445f-b124-fcacffa93067 Romera, F.J. (Francisco J.)|||/items/f0a732ab-66b2-4621-b4c7-c96b3a5a65e1 |
| author_role |
author |
| author2 |
Corpas, F.J. (Francisco Javier)|||/items/84be5386-440c-474a-a0fd-f67267dfe7ac Lucena, C. (Carlos)|||/items/b121bd8d-e0c2-4d88-b2c3-0ee61b13eb91 Alcántara, E. (Esteban)|||/items/929c81c3-fbff-409c-947b-b0116f5e2b16 Pérez-Vicente, R. (Rafael)|||/items/13f2ffef-e5ea-402a-8d47-31a9001c9c38 Zamarreño-Arregui, A.M. (Angel María)|||/items/316fe376-3b6c-4f68-b6da-03cf1fdab968 Bacaicoa, E. (Eva)|||/items/bf776d80-fd33-4c82-ae62-b50ca79eb262 García-Mina, J.M. (José María)|||/items/815053fc-2f39-4f00-aed0-c5f6ee370258 Bauer, P. (Petra)|||/items/3b95111e-a8e4-445f-b124-fcacffa93067 Romera, F.J. (Francisco J.)|||/items/f0a732ab-66b2-4621-b4c7-c96b3a5a65e1 |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Biología Vegetal y Animal, Ecología Ethylene Glutathione (GSH) Iron Long distance iron signal (LODIS) Nitric oxide (NO) Phloem S-nitrosoglutathione (GSNO) S-nitrosoglutathione reductase (GSNOR) |
| topic |
Biología Vegetal y Animal, Ecología Ethylene Glutathione (GSH) Iron Long distance iron signal (LODIS) Nitric oxide (NO) Phloem S-nitrosoglutathione (GSNO) S-nitrosoglutathione reductase (GSNOR) |
| description |
Ethylene, nitric oxide (NO) and glutathione (GSH) increase in Fe-deficient roots of Strategy I species where they participate in the up-regulation of Fe acquisition genes. However, S-nitrosoglutathione (GSNO), derived from NO and GSH, decreases in Fe-deficient roots. GSNO content is regulated by the GSNO-degrading enzyme S-nitrosoglutathione reductase (GSNOR). On the other hand, there are several results showing that the regulation of Fe acquisition genes does not solely depend on hormones and signaling molecules (such as ethylene or NO), which would act as activators, but also on the internal Fe content of plants, which would act as a repressor. Moreover, different results suggest that total Fe in roots is not the repressor of Fe acquisition genes, but rather the repressor is a Fe signal that moves from shoots to roots through the phloem [hereafter named LOng Distance Iron Signal (LODIS)]. To look further in the possible interactions between LODIS, ethylene and GSNOR, we compared Arabidopsis WT Columbia and LODIS-deficient mutant opt3-2 plants subjected to different Fe treatments that alter LODIS content. The opt3-2 mutant is impaired in the loading of shoot Fe into the phloem and presents constitutive expression of Fe acquisition genes. In roots of both Columbia and opt3-2 plants we determined 1-aminocyclopropane1-carboxylic acid (ACC, ethylene precursor), expression of ethylene synthesis and signaling genes, and GSNOR expression and activity. The results obtained showed that both 'ethylene' (ACC and the expression of ethylene synthesis and signaling genes) and 'GSNOR' (expression and activity) increased in Fe-deficient WT Columbia roots. Additionally, Fe-sufficient opt3-2 roots had higher 'ethylene' and 'GSNOR' than Fe-sufficient WT Columbia roots. The increase of both 'ethylene' and 'GSNOR' was not related to the total root Fe content but to the absence of a Fe shoot signal (LODIS), and was associated with the up-regulation of Fe acquisition genes. The possible relationship between GSNOR(GSNO) and ethylene is discussed. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-10-08 2018 2018-01-01 2018 2018-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/54578 |
| url |
https://hdl.handle.net/10171/54578 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15.301603 |