Development of 14 Microsatellite Markers in Odontites vernus s.l. (Orobanchaceae) and Cross-Amplification in Related Taxa

[EN] • Premise of the study: Microsatellite primers were developed for the fi rst time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent o...

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Detalles Bibliográficos
Autores: Pinto Carrasco, Daniel, Košnar, Jiří, López González, Noemí, Koutecký, Petr, Těšitel, Jakub, Rico Hernández, Enrique, Martínez Ortega, María Montserrat
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/140937
Acceso en línea:http://hdl.handle.net/10366/140937
Access Level:acceso abierto
Palabra clave:Grupo de Investigación en Biodiversidad, Sistemática y Conservación de Plantas Vasculares y Hongos
Conservation
Diploid-tetraploid complex
Microsatellite
Odontites vernus
Orobanchaceae
Rhinantheae
Plants
plantas
Descripción
Sumario:[EN] • Premise of the study: Microsatellite primers were developed for the fi rst time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene fl ow between different ploidy levels. • Methods and Results: Fourteen polymorphic and reproducible loci were identifi ed and optimized from O. vernus using a microsatellite-enriched library and 454 Junior sequencing. The set of primers amplifi ed di- to pentanucleotide repeats and showed two to 13 alleles per locus. Transferability was tested in 30 taxa (19 belonging to Odontites and 11 from eight other genera of Orobanchaceae tribe Rhinantheae). • Conclusions: The results indicate the utility of the newly developed microsatellites in O. vernus and several other species, which will be useful for taxon delimitation and conservation genetics studies.

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