Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development

The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have be...

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Detalhes bibliográficos
Autores: Steeman, Tomás José, Rubiolo Gaytán, Juan Andrés, Sánchez Piñón, Laura, Calcaterra, Nora B., Weiner, Andrea
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/26653
Acesso em linha:http://hdl.handle.net/10347/26653
Access Level:acceso abierto
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spelling Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell DevelopmentSteeman, Tomás JoséRubiolo Gaytán, Juan AndrésSánchez Piñón, LauraCalcaterra, Nora B.Weiner, AndreaThe neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differentiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3′UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expressionMDPIUniversidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10347/26653reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/266532026-06-15T12:47:27Z
dc.title.none.fl_str_mv Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
spellingShingle Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
Steeman, Tomás José
title_short Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_full Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_fullStr Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_full_unstemmed Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_sort Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
dc.creator.none.fl_str_mv Steeman, Tomás José
Rubiolo Gaytán, Juan Andrés
Sánchez Piñón, Laura
Calcaterra, Nora B.
Weiner, Andrea
author Steeman, Tomás José
author_facet Steeman, Tomás José
Rubiolo Gaytán, Juan Andrés
Sánchez Piñón, Laura
Calcaterra, Nora B.
Weiner, Andrea
author_role author
author2 Rubiolo Gaytán, Juan Andrés
Sánchez Piñón, Laura
Calcaterra, Nora B.
Weiner, Andrea
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
description The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differentiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3′UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expression
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/26653
url http://hdl.handle.net/10347/26653
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
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