Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases

Background: The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum beta-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified...

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Autores: Ruiz Del Castillo, B., Vinué, L. [0000-0001-7459-0814], Román, E.J., Guerra, B., Carattoli, A. [0000-0002-6120-6526], Torres, C. [0000-0003-3709-1690], Martínez-Martínez, L. [0000-0002-6091-4045]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc695cb750603269e819d9
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc695cb750603269e819d9
Access Level:acceso abierto
Palabra clave:E. coli
ESBL
Multiresistance
Plasmids
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spelling Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamasesRuiz Del Castillo, B.Vinué, L. [0000-0001-7459-0814]Román, E.J.Guerra, B.Carattoli, A. [0000-0002-6120-6526]Torres, C. [0000-0003-3709-1690]Martínez-Martínez, L. [0000-0002-6091-4045]E. coliESBLMultiresistancePlasmidsBackground: The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum beta-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing. Results: Both collections of organisms contained a large diversity of unrelated strains with some clones distributed in both groups of isolates. Large plasmids were identified in the two groups of organisms. Plasmids with replicons repK and repColE were more frequent among ESBL-producing isolates, while repFIA, repFII and repA/C replicons were more frequent in isolates lacking ESBL. Conjugative plasmids with repK and repA/C replicons coded for CTX-M-14 and CMY-2 beta-lactamases, respectively. No significant differences were observed in the distribution of class 1 and class 2 integrons among multiresistant E. coli producing or not ESBL, and dfrA17-ant(3[prime][prime])-Ie was the cassette arrangement most commonly found. Conclusions: In the concrete temporal and geographical context of this study, multiresistant E. coli producing ESBL or other mechanisms of resistance were largely clonally diverse and present some differences in the types of harboured plasmids. Still, some clones were found in both ESBL-producing and --lacking isolates.2013info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc695cb750603269e819d9reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-13-84info:eu-repo/semantics/altIdentifier/wos/WOS:000318623100001info:eu-repo/semantics/altIdentifier/pmid/23586437info:eu-repo/semantics/altIdentifier/pissn/1471-2180Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases, 2013, vol. 13, núm. 1, pág. 1-12info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc695cb750603269e819d92026-06-14T12:47:17Z
dc.title.none.fl_str_mv Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
title Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
spellingShingle Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
Ruiz Del Castillo, B.
E. coli
ESBL
Multiresistance
Plasmids
title_short Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
title_full Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
title_fullStr Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
title_full_unstemmed Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
title_sort Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases
dc.creator.none.fl_str_mv Ruiz Del Castillo, B.
Vinué, L. [0000-0001-7459-0814]
Román, E.J.
Guerra, B.
Carattoli, A. [0000-0002-6120-6526]
Torres, C. [0000-0003-3709-1690]
Martínez-Martínez, L. [0000-0002-6091-4045]
author Ruiz Del Castillo, B.
author_facet Ruiz Del Castillo, B.
Vinué, L. [0000-0001-7459-0814]
Román, E.J.
Guerra, B.
Carattoli, A. [0000-0002-6120-6526]
Torres, C. [0000-0003-3709-1690]
Martínez-Martínez, L. [0000-0002-6091-4045]
author_role author
author2 Vinué, L. [0000-0001-7459-0814]
Román, E.J.
Guerra, B.
Carattoli, A. [0000-0002-6120-6526]
Torres, C. [0000-0003-3709-1690]
Martínez-Martínez, L. [0000-0002-6091-4045]
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv E. coli
ESBL
Multiresistance
Plasmids
topic E. coli
ESBL
Multiresistance
Plasmids
description Background: The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum beta-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing. Results: Both collections of organisms contained a large diversity of unrelated strains with some clones distributed in both groups of isolates. Large plasmids were identified in the two groups of organisms. Plasmids with replicons repK and repColE were more frequent among ESBL-producing isolates, while repFIA, repFII and repA/C replicons were more frequent in isolates lacking ESBL. Conjugative plasmids with repK and repA/C replicons coded for CTX-M-14 and CMY-2 beta-lactamases, respectively. No significant differences were observed in the distribution of class 1 and class 2 integrons among multiresistant E. coli producing or not ESBL, and dfrA17-ant(3[prime][prime])-Ie was the cassette arrangement most commonly found. Conclusions: In the concrete temporal and geographical context of this study, multiresistant E. coli producing ESBL or other mechanisms of resistance were largely clonally diverse and present some differences in the types of harboured plasmids. Still, some clones were found in both ESBL-producing and --lacking isolates.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc695cb750603269e819d9
url https://investigacion.unirioja.es/documentos/5bbc695cb750603269e819d9
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-13-84
info:eu-repo/semantics/altIdentifier/wos/WOS:000318623100001
info:eu-repo/semantics/altIdentifier/pmid/23586437
info:eu-repo/semantics/altIdentifier/pissn/1471-2180
Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum beta-lactamases, 2013, vol. 13, núm. 1, pág. 1-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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