Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy

The cosmopolite Mesozoic fern Weichselia reticulata presents many peculiarities in the anatomy of its stem as well as in the architecture of its leaves. Previous analyses of the ultrastructure of their pinnule laminas provided some insight on xeromorphic traits. In this work new protocols for the pr...

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Detalles Bibliográficos
Autor: Blanco Moreno, Candela
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/715064
Acceso en línea:http://hdl.handle.net/10486/715064
https://dx.doi.org/10.7203/sjp.36.2.21300
Access Level:acceso abierto
Palabra clave:Bernissart
Las Hoyas
Leaf anatomy
Lower Cretaceous
Polypodiopsida
Biología y Biomedicina / Biología
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spelling Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomyBlanco Moreno, CandelaBernissartLas HoyasLeaf anatomyLower CretaceousPolypodiopsidaBiología y Biomedicina / BiologíaThe cosmopolite Mesozoic fern Weichselia reticulata presents many peculiarities in the anatomy of its stem as well as in the architecture of its leaves. Previous analyses of the ultrastructure of their pinnule laminas provided some insight on xeromorphic traits. In this work new protocols for the preparation of charred compression foliage specimens embedded in a limestone matrix for their observation under SEM are presented, which enhance the observation of different plant tissues. The preparation with HCl allows for the visualization of the whole lamina, from the upper to the lower epidermis, which had not been done to date. Additionally, collodion, latex, or wax coverings of the fossils before dissolving the matrix with HCl enhance the observation of different structures and prevent the fragmentation of the specimen. These techniques have permitted a better description of the vascular tissues, and a redescription of the stomata, which do not have subsidiary cells as previously described. Moreover, the traits observed suggest a closer systematic relationship of Weichselia reticulata with the Marattiales and that this fern lived in areas with fluctuating water availabilityThis work was performed with the support of the Sociedad Española de Paleontología through the 2019 call of the grant ‘Ayudas a Jóvenes Investigadores de la Sociedad Española de Paleontología’. The results of this work are part of the PID2019-105546GB-I00 project from the Ministerio de Ciencia, e Innovación de EspañaSociedad Española de PaleontologíaDepartamento de BiologíaFacultad de Ciencias20212021-07-13research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/715064https://dx.doi.org/10.7203/sjp.36.2.21300reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7150642026-06-23T12:46:27Z
dc.title.none.fl_str_mv Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
title Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
spellingShingle Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
Blanco Moreno, Candela
Bernissart
Las Hoyas
Leaf anatomy
Lower Cretaceous
Polypodiopsida
Biología y Biomedicina / Biología
title_short Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
title_full Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
title_fullStr Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
title_full_unstemmed Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
title_sort Preparation protocols for SEM visualization of charred fossil plants: the case of Weichselia reticulata pinnule anatomy
dc.creator.none.fl_str_mv Blanco Moreno, Candela
author Blanco Moreno, Candela
author_facet Blanco Moreno, Candela
author_role author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Bernissart
Las Hoyas
Leaf anatomy
Lower Cretaceous
Polypodiopsida
Biología y Biomedicina / Biología
topic Bernissart
Las Hoyas
Leaf anatomy
Lower Cretaceous
Polypodiopsida
Biología y Biomedicina / Biología
description The cosmopolite Mesozoic fern Weichselia reticulata presents many peculiarities in the anatomy of its stem as well as in the architecture of its leaves. Previous analyses of the ultrastructure of their pinnule laminas provided some insight on xeromorphic traits. In this work new protocols for the preparation of charred compression foliage specimens embedded in a limestone matrix for their observation under SEM are presented, which enhance the observation of different plant tissues. The preparation with HCl allows for the visualization of the whole lamina, from the upper to the lower epidermis, which had not been done to date. Additionally, collodion, latex, or wax coverings of the fossils before dissolving the matrix with HCl enhance the observation of different structures and prevent the fragmentation of the specimen. These techniques have permitted a better description of the vascular tissues, and a redescription of the stomata, which do not have subsidiary cells as previously described. Moreover, the traits observed suggest a closer systematic relationship of Weichselia reticulata with the Marattiales and that this fern lived in areas with fluctuating water availability
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-13
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/715064
https://dx.doi.org/10.7203/sjp.36.2.21300
url http://hdl.handle.net/10486/715064
https://dx.doi.org/10.7203/sjp.36.2.21300
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedad Española de Paleontología
publisher.none.fl_str_mv Sociedad Española de Paleontología
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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