Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey
BACKGROUND: Intestinal protozoan parasites are major contributors to the global burden of gastrointestinal disease causing significant socioeconomic consequences. Children living in resource-poor settings with restricted access to water and sanitary services are particularly at risk of these infecti...
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/7597 |
| Acesso em linha: | http://hdl.handle.net/20.500.12105/7597 |
| Access Level: | acceso abierto |
| Palavra-chave: | Adolescent Adult Aged Blastocystis Blastocystis Infections Brazil Child Child, Preschool Cross-Sectional Studies DNA, Ribosomal Feces Female Genetic Variation Giardia lamblia Giardiasis Humans Infant Infant, Newborn Intestinal Diseases, Parasitic Male Middle Aged Prevalence Prospective Studies Residence Characteristics |
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Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community surveySeguí, RaimundoMuñoz-Antoli, CarlaKlisiowicz, Debora ROishi, Camila YKöster, Pamela Carolinade Lucio, AidaHernandez-De-Mingo, MartaPuente, PaulaToledo, RafaelEsteban, José GCarmena, DavidAdolescentAdultAgedBlastocystisBlastocystis InfectionsBrazilChildChild, PreschoolCross-Sectional StudiesDNA, RibosomalFecesFemaleGenetic VariationGiardia lambliaGiardiasisHumansInfantInfant, NewbornIntestinal Diseases, ParasiticMaleMiddle AgedPrevalenceProspective StudiesResidence CharacteristicsBACKGROUND: Intestinal protozoan parasites are major contributors to the global burden of gastrointestinal disease causing significant socioeconomic consequences. Children living in resource-poor settings with restricted access to water and sanitary services are particularly at risk of these infections. METHODS: A prospective, community-based, cross-sectional survey was conducted in Paraná (southern Brazil) between May 2015 and May 2016. A total of 766 stool samples were individually collected from volunteers (male/female ratio: 0.99; age range: 0-76 years) and used for investigating the presence of intestinal helminth and protozoan species by routine microscopic procedures including the Kato-Katz and modified Ritchie concentration methods and the Ziehl-Neelsen stain technique. Quantitative real-time PCR confirmed microscopy-positive samples for Giardia duodenalis and the assemblages and sub-assemblages determined by multilocus sequence-based genotyping of the glutamate dehydrogenase (gdh) and β-giardin (bg) genes of the parasite. Identification of Blastocystis subtypes was carried out by amplification and sequencing of a partial fragment of the small-subunit ribosomal RNA gene (SSU rDNA) of this heterokont microorganism. RESULTS: Overall, 46.1% (353/766) of the participants were infected/colonised by at least one intestinal parasite/commensal species. Protozoan and helminth species were detected in 42.7% and 10.1% of the surveyed population, respectively. Blastocystis sp. (28.2%), Endolimax nana (14.9%), and Giardia duodenalis (11.0%) were the most prevalent species found among protozoans and Ascaris lumbricoides (5.0%), Trichuris trichiura (4.6%) and hookworms (1.0%) among helminths. A total of 38 G. duodenalis-positive samples were genotyped at gdh and bg markers, revealing the presence of the sub-assemblages AII (47.4%), AII/AIII (2.6%), BIII (5.3%), BIV (26.3%) and BIII/BIV (13.1%). Two samples (5.3%) were only identified as assemblage B. AII was predominantly found in females aged 5-9 years and was associated with a higher likelihood of reporting gastrointestinal symptoms. A total of 102 Blastocystis-positive samples were successfully subtyped at the SSU rRNA gene revealing the presence of ST1 (36.3%), ST2 (15.7%), ST3 (41.2%), ST4 (2.9%), ST6 (1.0%) and ST8 (2.9%). CONCLUSIONS: Data presented here indicate that enteric parasites still represent a pressing health concern in Paraná, Brazil, probably due to sub-optimal water, sanitation and hygiene conditions. A mostly anthroponotic origin is suspected for G. duodenalis and Blastocystis sp. infections.BioMed Central (BMC)Instituto de Salud Carlos IIIMinisterio de Economía y Competitividad (España)RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)20192019-05-2020182018-08-3020182018-08-30journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/7597reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/75972026-06-12T12:43:37Z |
| dc.title.none.fl_str_mv |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| title |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| spellingShingle |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey Seguí, Raimundo Adolescent Adult Aged Blastocystis Blastocystis Infections Brazil Child Child, Preschool Cross-Sectional Studies DNA, Ribosomal Feces Female Genetic Variation Giardia lamblia Giardiasis Humans Infant Infant, Newborn Intestinal Diseases, Parasitic Male Middle Aged Prevalence Prospective Studies Residence Characteristics |
| title_short |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| title_full |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| title_fullStr |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| title_full_unstemmed |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| title_sort |
Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey |
| dc.creator.none.fl_str_mv |
Seguí, Raimundo Muñoz-Antoli, Carla Klisiowicz, Debora R Oishi, Camila Y Köster, Pamela Carolina de Lucio, Aida Hernandez-De-Mingo, Marta Puente, Paula Toledo, Rafael Esteban, José G Carmena, David |
| author |
Seguí, Raimundo |
| author_facet |
Seguí, Raimundo Muñoz-Antoli, Carla Klisiowicz, Debora R Oishi, Camila Y Köster, Pamela Carolina de Lucio, Aida Hernandez-De-Mingo, Marta Puente, Paula Toledo, Rafael Esteban, José G Carmena, David |
| author_role |
author |
| author2 |
Muñoz-Antoli, Carla Klisiowicz, Debora R Oishi, Camila Y Köster, Pamela Carolina de Lucio, Aida Hernandez-De-Mingo, Marta Puente, Paula Toledo, Rafael Esteban, José G Carmena, David |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Salud Carlos III Ministerio de Economía y Competitividad (España) RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) |
| dc.subject.none.fl_str_mv |
Adolescent Adult Aged Blastocystis Blastocystis Infections Brazil Child Child, Preschool Cross-Sectional Studies DNA, Ribosomal Feces Female Genetic Variation Giardia lamblia Giardiasis Humans Infant Infant, Newborn Intestinal Diseases, Parasitic Male Middle Aged Prevalence Prospective Studies Residence Characteristics |
| topic |
Adolescent Adult Aged Blastocystis Blastocystis Infections Brazil Child Child, Preschool Cross-Sectional Studies DNA, Ribosomal Feces Female Genetic Variation Giardia lamblia Giardiasis Humans Infant Infant, Newborn Intestinal Diseases, Parasitic Male Middle Aged Prevalence Prospective Studies Residence Characteristics |
| description |
BACKGROUND: Intestinal protozoan parasites are major contributors to the global burden of gastrointestinal disease causing significant socioeconomic consequences. Children living in resource-poor settings with restricted access to water and sanitary services are particularly at risk of these infections. METHODS: A prospective, community-based, cross-sectional survey was conducted in Paraná (southern Brazil) between May 2015 and May 2016. A total of 766 stool samples were individually collected from volunteers (male/female ratio: 0.99; age range: 0-76 years) and used for investigating the presence of intestinal helminth and protozoan species by routine microscopic procedures including the Kato-Katz and modified Ritchie concentration methods and the Ziehl-Neelsen stain technique. Quantitative real-time PCR confirmed microscopy-positive samples for Giardia duodenalis and the assemblages and sub-assemblages determined by multilocus sequence-based genotyping of the glutamate dehydrogenase (gdh) and β-giardin (bg) genes of the parasite. Identification of Blastocystis subtypes was carried out by amplification and sequencing of a partial fragment of the small-subunit ribosomal RNA gene (SSU rDNA) of this heterokont microorganism. RESULTS: Overall, 46.1% (353/766) of the participants were infected/colonised by at least one intestinal parasite/commensal species. Protozoan and helminth species were detected in 42.7% and 10.1% of the surveyed population, respectively. Blastocystis sp. (28.2%), Endolimax nana (14.9%), and Giardia duodenalis (11.0%) were the most prevalent species found among protozoans and Ascaris lumbricoides (5.0%), Trichuris trichiura (4.6%) and hookworms (1.0%) among helminths. A total of 38 G. duodenalis-positive samples were genotyped at gdh and bg markers, revealing the presence of the sub-assemblages AII (47.4%), AII/AIII (2.6%), BIII (5.3%), BIV (26.3%) and BIII/BIV (13.1%). Two samples (5.3%) were only identified as assemblage B. AII was predominantly found in females aged 5-9 years and was associated with a higher likelihood of reporting gastrointestinal symptoms. A total of 102 Blastocystis-positive samples were successfully subtyped at the SSU rRNA gene revealing the presence of ST1 (36.3%), ST2 (15.7%), ST3 (41.2%), ST4 (2.9%), ST6 (1.0%) and ST8 (2.9%). CONCLUSIONS: Data presented here indicate that enteric parasites still represent a pressing health concern in Paraná, Brazil, probably due to sub-optimal water, sanitation and hygiene conditions. A mostly anthroponotic origin is suspected for G. duodenalis and Blastocystis sp. infections. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-08-30 2018 2018-08-30 2019 2019-05-20 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12105/7597 |
| url |
http://hdl.handle.net/20.500.12105/7597 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central (BMC) |
| publisher.none.fl_str_mv |
BioMed Central (BMC) |
| dc.source.none.fl_str_mv |
reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII) |
| instname_str |
Instituto de Salud Carlos III (ISCIII) |
| reponame_str |
Repisalud |
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Repisalud |
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|
| repository.mail.fl_str_mv |
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1869415539352272896 |
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15,198674 |