Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages

Objectives: To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles Methods: Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-l...

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Autores: Rodríguez-Rubio, Lorena, Serna, Carlos, Ares-Arroyo, Manuel, Matamoros, Bosco R., Delgado-Blas, Jose F., Montero, Natalia, Bernabe-Balas, Cristina, Wedel, Emilia F., Mendez, Irene S., Muniesa Pérez, Ma. Teresa, Gonzalez-Zorn, Bruno
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/191800
Acesso em linha:https://hdl.handle.net/2445/191800
Access Level:acceso abierto
Palavra-chave:Plasmidis
Bacteris
Plasmids
Bacteria
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spelling Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phagesRodríguez-Rubio, LorenaSerna, CarlosAres-Arroyo, ManuelMatamoros, Bosco R.Delgado-Blas, Jose F.Montero, NataliaBernabe-Balas, CristinaWedel, Emilia F.Mendez, Irene S.Muniesa Pérez, Ma. TeresaGonzalez-Zorn, BrunoPlasmidisBacterisPlasmidsBacteriaObjectives: To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles Methods: Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting. Results: Multicopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10000 times more frequent than when it was encoded by a large plasmid with a low copy number. Conclusions: Multicopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation.Oxford University Press2022202220202022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/191800Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1093/jac/dkaa311Journal of Antimicrobial Chemotherapy, 2020, vol. 75, num. 11, p. 3173-3180https://doi.org/10.1093/jac/dkaa311info:eu-repo/grantAgreement/EC/H2020/765147info:eu-repo/grantAgreement/EC/H2020/773830cc by-nc (c) Rodríguez-Rubio, Lorena et al., 2020http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1918002026-05-29T05:05:01Z
dc.title.none.fl_str_mv Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
title Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
spellingShingle Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
Rodríguez-Rubio, Lorena
Plasmidis
Bacteris
Plasmids
Bacteria
title_short Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
title_full Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
title_fullStr Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
title_full_unstemmed Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
title_sort Extensive antimicrobial resistance mobilization via Multicopy Plasmid Encapsidation mediated by temperate phages
dc.creator.none.fl_str_mv Rodríguez-Rubio, Lorena
Serna, Carlos
Ares-Arroyo, Manuel
Matamoros, Bosco R.
Delgado-Blas, Jose F.
Montero, Natalia
Bernabe-Balas, Cristina
Wedel, Emilia F.
Mendez, Irene S.
Muniesa Pérez, Ma. Teresa
Gonzalez-Zorn, Bruno
author Rodríguez-Rubio, Lorena
author_facet Rodríguez-Rubio, Lorena
Serna, Carlos
Ares-Arroyo, Manuel
Matamoros, Bosco R.
Delgado-Blas, Jose F.
Montero, Natalia
Bernabe-Balas, Cristina
Wedel, Emilia F.
Mendez, Irene S.
Muniesa Pérez, Ma. Teresa
Gonzalez-Zorn, Bruno
author_role author
author2 Serna, Carlos
Ares-Arroyo, Manuel
Matamoros, Bosco R.
Delgado-Blas, Jose F.
Montero, Natalia
Bernabe-Balas, Cristina
Wedel, Emilia F.
Mendez, Irene S.
Muniesa Pérez, Ma. Teresa
Gonzalez-Zorn, Bruno
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plasmidis
Bacteris
Plasmids
Bacteria
topic Plasmidis
Bacteris
Plasmids
Bacteria
description Objectives: To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles Methods: Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting. Results: Multicopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10000 times more frequent than when it was encoded by a large plasmid with a low copy number. Conclusions: Multicopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191800
url https://hdl.handle.net/2445/191800
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1093/jac/dkaa311
Journal of Antimicrobial Chemotherapy, 2020, vol. 75, num. 11, p. 3173-3180
https://doi.org/10.1093/jac/dkaa311
info:eu-repo/grantAgreement/EC/H2020/765147
info:eu-repo/grantAgreement/EC/H2020/773830
dc.rights.none.fl_str_mv cc by-nc (c) Rodríguez-Rubio, Lorena et al., 2020
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc (c) Rodríguez-Rubio, Lorena et al., 2020
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415539318718464
score 15,812429

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