Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment

Approximately 10,000 different dyes and pigments are produced annually worldwide and used extensively in the dye and printing industries. This has resulted in the generation of large volumes of highly polluted wastewater. Apart from the aesthetic deterioration of the natural water bodies, dyes also...

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Detalhes bibliográficos
Autor: Osma Cruz, Johann Faccelo
Formato: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/8578
Acesso em linha:http://www.tdx.cat/TDX-1217109-123426
http://hdl.handle.net/10803/8578
Access Level:acceso abierto
Palavra-chave:white-rot fungi
dye treatment
immobilization
Laccase
54
579
66
id ES_a4b72e508debfad660d06eb23c1dfbf5
oai_identifier_str oai:www.tdx.cat:10803/8578
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
title Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
spellingShingle Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
Osma Cruz, Johann Faccelo
white-rot fungi
dye treatment
immobilization
Laccase
54
579
66
title_short Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
title_full Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
title_fullStr Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
title_full_unstemmed Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
title_sort Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatment
dc.creator.none.fl_str_mv Osma Cruz, Johann Faccelo
author Osma Cruz, Johann Faccelo
author_facet Osma Cruz, Johann Faccelo
author_role author
dc.contributor.none.fl_str_mv Rodríguez Couto, Susana
Toca Herrera, José Luis
Fabregat i Llangostera, Azael
Universitat Rovira i Virgili. Departament d'Enginyeria Química
dc.subject.none.fl_str_mv white-rot fungi
dye treatment
immobilization
Laccase
54
579
66
topic white-rot fungi
dye treatment
immobilization
Laccase
54
579
66
description Approximately 10,000 different dyes and pigments are produced annually worldwide and used extensively in the dye and printing industries. This has resulted in the generation of large volumes of highly polluted wastewater. Apart from the aesthetic deterioration of the natural water bodies, dyes also cause harm to the flora and fauna in the natural environment. Therefore, wastewater containing dyes must be treated prior to their discharge into the environment.<br/><br/>Different methods can be applied for the treatment of synthetic dyes from aqueous solutions, such as ozonation, coagulation, flocculation, reverse osmosis and adsorption. However, biological treatments are promising alternatives with different approaches going from the complete immobilization of microorganisms to the pure enzyme utilization. Among all enzymes, laccases are an interesting alternative for the dye degradation due to their low affinity and wide specificity for the substrates. Laccases are multicopper oxidases found in higher plant and microorganisms, like white-rot-fungi; and carry out one-electron oxidation of phenolic and related compounds, and reduce O2 to water. <br/><br/>Thus, this work proposes different strategies based on the use of laccases for the discoloration of synthetic dyes from aqueous solutions. These strategies include studies in different fields to promote eco-friendly solutions for different assets of the whole process. These studies include: the selection of substrates for the production of laccase by the white-rot fungus Trametes pubescens, the possible reutilization of these substrates in the discoloration process, the optimization of the laccase production per culture, the scale up of the laccase production, the use of free and immobilized laccase in the discoloration of dyes and the use of different immobilization techniques to increase the reutilization of the immobilized laccase for the treatment of synthetic dyes.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009
2009
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-1217109-123426
http://hdl.handle.net/10803/8578
url http://www.tdx.cat/TDX-1217109-123426
http://hdl.handle.net/10803/8578
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Rovira i Virgili
publisher.none.fl_str_mv Universitat Rovira i Virgili
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Production of laccases by the white-rot fungus trametes pubescens for their potential application to synthetic dye treatmentOsma Cruz, Johann Faccelowhite-rot fungidye treatmentimmobilizationLaccase5457966Approximately 10,000 different dyes and pigments are produced annually worldwide and used extensively in the dye and printing industries. This has resulted in the generation of large volumes of highly polluted wastewater. Apart from the aesthetic deterioration of the natural water bodies, dyes also cause harm to the flora and fauna in the natural environment. Therefore, wastewater containing dyes must be treated prior to their discharge into the environment.<br/><br/>Different methods can be applied for the treatment of synthetic dyes from aqueous solutions, such as ozonation, coagulation, flocculation, reverse osmosis and adsorption. However, biological treatments are promising alternatives with different approaches going from the complete immobilization of microorganisms to the pure enzyme utilization. Among all enzymes, laccases are an interesting alternative for the dye degradation due to their low affinity and wide specificity for the substrates. Laccases are multicopper oxidases found in higher plant and microorganisms, like white-rot-fungi; and carry out one-electron oxidation of phenolic and related compounds, and reduce O2 to water. <br/><br/>Thus, this work proposes different strategies based on the use of laccases for the discoloration of synthetic dyes from aqueous solutions. These strategies include studies in different fields to promote eco-friendly solutions for different assets of the whole process. These studies include: the selection of substrates for the production of laccase by the white-rot fungus Trametes pubescens, the possible reutilization of these substrates in the discoloration process, the optimization of the laccase production per culture, the scale up of the laccase production, the use of free and immobilized laccase in the discoloration of dyes and the use of different immobilization techniques to increase the reutilization of the immobilized laccase for the treatment of synthetic dyes.Aproximadamente 10,000 tintes y pigmentos diferentes son producidos anualmente y tienen un uso extendido en las industrias de teñido e impresión. Esto ha generado grandes cantidades de aguas residuales altamente contaminadas. A parte del deterioro estético que sufren los cuerpos de agua, los tintes también causan daño a la flora y fauna presentes en el medio ambiente. Por ello, las aguas residuales que contienen tintes deben ser tratadas antes de su descarga al ambiente.<br/>Distintos métodos pueden ser empleados para el tratamiento de tintes sintéticos en soluciones acuosas, tales como la ozonización, coagulación, floculación, osmosis inversa y la adsorción. Sin embargo, los tratamientos biológicos resultan una alternativa prometedora con distintas aproximaciones que van desde la inmovilización de microorganismos hasta el uso de las enzimas puras. Entre todas estas enzimas, las lacasas resultan ser muy interesantes para la degradación de tintes debido a su baja afinidad y amplia especifidad por los substratos. Las lacasas son oxidasas de multicobre que se encuentran en plantas, microorganismos, como los hongos de putrefacción-blanca, y llevan a cabo la oxidación del fenol y compuestos relacionados, y reducen el O2 a agua.<br/>Debido a esto, en este trabajo se proponen distintas estrategias basadas en la utilización de las lacasas para la decoloración de tintes sintéticos en medios acuosos. Estas estrategias incluyen estudios en distintas áreas para promover soluciones amigables con el medio ambiente en las distintas etapas del proceso. Estos estudios incluyen: la selección de substratos para la producción de lacasa con el hongo de putrefacción-blanca Trametes pubescens, la posible reutilización de estos substratos en los procesos de decoloración, la optimización de la producción de la lacasa por cultivo, el escalado de la producción de la lacasa, el uso de lacasa libre e inmovilizada en la decoloración de tintes y el uso de distintas técnicas de inmovilización para incrementar la reutilización de la lacasa inmovilizada en el tratamiento de tintes sintéticos.Universitat Rovira i VirgiliRodríguez Couto, SusanaToca Herrera, José LuisFabregat i Llangostera, AzaelUniversitat Rovira i Virgili. Departament d'Enginyeria Química2011200920092009info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-1217109-123426http://hdl.handle.net/10803/8578TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/85782026-06-14T12:46:07Z
score 15,300724

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