Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate

Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to un...

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Authors: Perlas Puente, Albert, Bertran, Kateri, Abad Morejón de Girón, Xavier, Borrego, Carles M., Nofrarias Espadamala, Miquel, Valle, Rosa, Pailler García, Lola, Ramis, Antonio, Cortey, Martí, Acuña, Vicenç, Majo, Natalia
Format: article
Publication Date:2022
Country:España
Institution:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repository:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/2085
Online Access:http://hdl.handle.net/20.500.12327/2085
https://doi.org/10.1016/j.scitotenv.2022.160902
Access Level:Open access
Keyword:619
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spelling Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climatePerlas Puente, AlbertBertran, KateriAbad Morejón de Girón, XavierBorrego, Carles M.Nofrarias Espadamala, MiquelValle, RosaPailler García, LolaRamis, AntonioCortey, MartíAcuña, VicençMajo, Natalia619Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i.e., 10–13 °C vs. 16–18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e.g., weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalSanitat Animal202320242022info:eu-repo/semantics/article32application/pdfhttp://hdl.handle.net/20.500.12327/2085https://doi.org/10.1016/j.scitotenv.2022.160902reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésScience of the Total EnvironmentINIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00088-C03-03/ES/Influenza aviar: Estudio de la dinámica de la infección en aves autóctonas y de persistencia del virus en condiciones ambientales/MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-114060RR-C33/ES/INVESTIGANDO LOS MECANISMOS MOLECULARES DE LA EVOLUCION DE LA INFECCION CON VIRUS DE INFLUENZA AVIAR EN LAS AVES MEDIANTE UNA APROXIMACION MULTI-OMICA/INFLUOMAinfo:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/20852026-06-16T08:51:17Z
dc.title.none.fl_str_mv Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
title Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
spellingShingle Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
Perlas Puente, Albert
619
title_short Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
title_full Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
title_fullStr Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
title_full_unstemmed Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
title_sort Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
dc.creator.none.fl_str_mv Perlas Puente, Albert
Bertran, Kateri
Abad Morejón de Girón, Xavier
Borrego, Carles M.
Nofrarias Espadamala, Miquel
Valle, Rosa
Pailler García, Lola
Ramis, Antonio
Cortey, Martí
Acuña, Vicenç
Majo, Natalia
author Perlas Puente, Albert
author_facet Perlas Puente, Albert
Bertran, Kateri
Abad Morejón de Girón, Xavier
Borrego, Carles M.
Nofrarias Espadamala, Miquel
Valle, Rosa
Pailler García, Lola
Ramis, Antonio
Cortey, Martí
Acuña, Vicenç
Majo, Natalia
author_role author
author2 Bertran, Kateri
Abad Morejón de Girón, Xavier
Borrego, Carles M.
Nofrarias Espadamala, Miquel
Valle, Rosa
Pailler García, Lola
Ramis, Antonio
Cortey, Martí
Acuña, Vicenç
Majo, Natalia
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i.e., 10–13 °C vs. 16–18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e.g., weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2085
https://doi.org/10.1016/j.scitotenv.2022.160902
url http://hdl.handle.net/20.500.12327/2085
https://doi.org/10.1016/j.scitotenv.2022.160902
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Science of the Total Environment
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00088-C03-03/ES/Influenza aviar: Estudio de la dinámica de la infección en aves autóctonas y de persistencia del virus en condiciones ambientales/
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-114060RR-C33/ES/INVESTIGANDO LOS MECANISMOS MOLECULARES DE LA EVOLUCION DE LA INFECCION CON VIRUS DE INFLUENZA AVIAR EN LAS AVES MEDIANTE UNA APROXIMACION MULTI-OMICA/INFLUOMA
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 32
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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