Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification
Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables vis...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/23218 |
| Acceso en línea: | https://hdl.handle.net/20.500.12105/23218 |
| Access Level: | acceso abierto |
| Palabra clave: | COVID-19 Nucleocapsid primers ORF1ab primers SARS-CoV-2 detection Virus LAMP amplification Prueba de Ácido Nucleico para COVID-19 Colorimetría Humanos SARS-CoV-2 Proteínas Virales Cartilla de ADN Técnicas de Diagnóstico Molecular Poliproteínas Técnicas de Amplificación de Ácido Nucleico Nucleic Acid Amplification Techniques Polyproteins Colorimetry DNA Primers Humans Molecular Diagnostic Techniques Viral Proteins COVID-19 Nucleic Acid Testing |
| Sumario: | Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection. METHOD SUMMARY Optimized loop-mediated isothermal amplification reactions were carried out using the combination of two sets of primers (designed on ORF1ab and N sequences). Saliva samples were pretreated by heating and proteinase K, before SARS-CoV-2 determination by loop-mediated isothermal amplification. |
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