Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation

Reductions in fish oil content in aquafeeds can generate physiological and immunological disorders in farmed fish, associated to low dietary levels of n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFAs) and increased levels of n-6 PUFAs. Based on mammalian literature, spices could potentially...

Descripción completa

Detalles Bibliográficos
Autores: Ruiz, Alberto, Sanahuja, Ignasi, Andree, Karl B., Furones, Dolors, Holorea, Paul G., Calduch-Giner, Josep A., Pastor, Jose J., Viñas, Marc, Pérez-Sánchez, Jaume, Morais, Sofia, Gisbert, Enric
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/2538
Acceso en línea:http://hdl.handle.net/20.500.12327/2538
https://doi.org/10.1016/j.aquaculture.2023.740378
Access Level:acceso abierto
Palabra clave:637
id ES_a4ad104fdbc8aa7fda91f65332eaf51b
oai_identifier_str oai:recercat.cat:20.500.12327/2538
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
title Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
spellingShingle Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
Ruiz, Alberto
637
title_short Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
title_full Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
title_fullStr Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
title_full_unstemmed Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
title_sort Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulation
dc.creator.none.fl_str_mv Ruiz, Alberto
Sanahuja, Ignasi
Andree, Karl B.
Furones, Dolors
Holorea, Paul G.
Calduch-Giner, Josep A.
Pastor, Jose J.
Viñas, Marc
Pérez-Sánchez, Jaume
Morais, Sofia
Gisbert, Enric
author Ruiz, Alberto
author_facet Ruiz, Alberto
Sanahuja, Ignasi
Andree, Karl B.
Furones, Dolors
Holorea, Paul G.
Calduch-Giner, Josep A.
Pastor, Jose J.
Viñas, Marc
Pérez-Sánchez, Jaume
Morais, Sofia
Gisbert, Enric
author_role author
author2 Sanahuja, Ignasi
Andree, Karl B.
Furones, Dolors
Holorea, Paul G.
Calduch-Giner, Josep A.
Pastor, Jose J.
Viñas, Marc
Pérez-Sánchez, Jaume
Morais, Sofia
Gisbert, Enric
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
Sostenibilitat en Biosistemes
dc.subject.none.fl_str_mv 637
topic 637
description Reductions in fish oil content in aquafeeds can generate physiological and immunological disorders in farmed fish, associated to low dietary levels of n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFAs) and increased levels of n-6 PUFAs. Based on mammalian literature, spices could potentially counteract some of these negative effects. To explore this hypothesis, this work intended to offer a holistic insight on the effect of supplementing a diet (44% crude protein, 18% crude fat, 21.4 MJ kg−1 gross energy) containing low (3%) fish oil and poultry fat as the main oil source (43% of added oils) with 0.2% of an encapsulated combination of spice oleoresins (SO) including turmeric, capsicum, black pepper, and ginger in gilthead seabream (Sparus aurata, 44.1 ± 4.1 g). At the end of the trial (90 days), fish fed with the SO diet displayed lower levels of perivisceral and hepatic fat, as well as reduced accumulation of lipid deposits, assessed histologically, in liver and intestine. These findings were consistent with an increased activity of bile salt-activated lipase in the anterior intestine and the regulation of gene expression (qPCR) of several biomarkers related to lipid metabolism, including several key metabolic sensors and nuclear factors (hnf4a and srebp2 at 2 h after feeding and lxrα, fxr, pparγ, pparβ, sirt1 in 48 h-fasted fish) that coordinate the expression of a network of genes (many of them significantly affected herein) regulating lipid, cholesterol and bile acid homeostasis. Moreover, the combination of SO had an immunomodulatory effect in the intestine which reached its climax in 48 h fasted-fish with the down-regulation of the pro-inflammatory cytokines tnf-α, il-12β, il-15, il-34 and cell markers cd4–1, cd8b, and ccr11, suggesting a potential anti-inflammatory response. No major effects were noted on fish growth and feed performance, on the oxidative status of the fish (although there were some subtle indications of reduced hepatic ROS levels). When assessing the gut microbial communities of gilthead seabream (16S rRNA gene sequencing on Illumina MiSeq) there were no differences in alpha and beta diversity between both experimental diets in the anterior and posterior intestines, except for an increase in the relative abundance of Bacteroides, Desulfovibrio, Candidatus Arthromitus, and Ralstonia. In conclusion, supplementation of diets with the tested combination of SO may be an effective strategy to prevent lipid accumulation and inflammation in fish fed diets containing reduced fish oil levels.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2538
https://doi.org/10.1016/j.aquaculture.2023.740378
url http://hdl.handle.net/20.500.12327/2538
https://doi.org/10.1016/j.aquaculture.2023.740378
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquaculture
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095653-R-I00/ES/La dieta y microbiota intestinal como herramientas para regular la adiposidad en peces/ADIPOQUIZ
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC2020-043933-I/ES/ /
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 40
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415517118267392
spelling Supplementation of gilthead seabream (Sparus aurata) diets with spices as a functional strategy to control excess adiposity through lipid, cholesterol and bile acid metabolism, and to induce an immunomodulatory intestinal regulationRuiz, AlbertoSanahuja, IgnasiAndree, Karl B.Furones, DolorsHolorea, Paul G.Calduch-Giner, Josep A.Pastor, Jose J.Viñas, MarcPérez-Sánchez, JaumeMorais, SofiaGisbert, Enric637Reductions in fish oil content in aquafeeds can generate physiological and immunological disorders in farmed fish, associated to low dietary levels of n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFAs) and increased levels of n-6 PUFAs. Based on mammalian literature, spices could potentially counteract some of these negative effects. To explore this hypothesis, this work intended to offer a holistic insight on the effect of supplementing a diet (44% crude protein, 18% crude fat, 21.4 MJ kg−1 gross energy) containing low (3%) fish oil and poultry fat as the main oil source (43% of added oils) with 0.2% of an encapsulated combination of spice oleoresins (SO) including turmeric, capsicum, black pepper, and ginger in gilthead seabream (Sparus aurata, 44.1 ± 4.1 g). At the end of the trial (90 days), fish fed with the SO diet displayed lower levels of perivisceral and hepatic fat, as well as reduced accumulation of lipid deposits, assessed histologically, in liver and intestine. These findings were consistent with an increased activity of bile salt-activated lipase in the anterior intestine and the regulation of gene expression (qPCR) of several biomarkers related to lipid metabolism, including several key metabolic sensors and nuclear factors (hnf4a and srebp2 at 2 h after feeding and lxrα, fxr, pparγ, pparβ, sirt1 in 48 h-fasted fish) that coordinate the expression of a network of genes (many of them significantly affected herein) regulating lipid, cholesterol and bile acid homeostasis. Moreover, the combination of SO had an immunomodulatory effect in the intestine which reached its climax in 48 h fasted-fish with the down-regulation of the pro-inflammatory cytokines tnf-α, il-12β, il-15, il-34 and cell markers cd4–1, cd8b, and ccr11, suggesting a potential anti-inflammatory response. No major effects were noted on fish growth and feed performance, on the oxidative status of the fish (although there were some subtle indications of reduced hepatic ROS levels). When assessing the gut microbial communities of gilthead seabream (16S rRNA gene sequencing on Illumina MiSeq) there were no differences in alpha and beta diversity between both experimental diets in the anterior and posterior intestines, except for an increase in the relative abundance of Bacteroides, Desulfovibrio, Candidatus Arthromitus, and Ralstonia. In conclusion, supplementation of diets with the tested combination of SO may be an effective strategy to prevent lipid accumulation and inflammation in fish fed diets containing reduced fish oil levels.This work has been financed through the ADIPOQUIZ project (RTI2018-095653-R-I00) funded by the Ministerio de Ciencia, Innovación y Universidades (Spain). Alberto Ruiz was supported by a predoctoral grant (PRE2019-091259) linked to the ADIPOQUIZ project. Ignasi Sanahuja was supported by a Juan de la Cierva postdoctoral contract (FJC2020-043933-I). Authors would like to express their gratitude to Magda Monllaó, Pau Sanchez, Esteban Hernandez, Marta Sastre, Maria Curto, Olga Bellot and Edgar Bertomeu, staff from IRTA La Ràpita, for their dedication and valuable assistance during the execution of the experiment, Miriam Guivernau from IRTA Torre Marimon for her aid in microbial analysis, and María Angeles González from IATS, CSIC for her assistance on PCR analyses.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalAqüiculturaSostenibilitat en Biosistemes2023info:eu-repo/semantics/article40http://hdl.handle.net/20.500.12327/2538https://doi.org/10.1016/j.aquaculture.2023.740378reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAquacultureMICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095653-R-I00/ES/La dieta y microbiota intestinal como herramientas para regular la adiposidad en peces/ADIPOQUIZMICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC2020-043933-I/ES/ /Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/25382026-05-29T05:05:01Z
score 15,811543

Similares en LA Referencia