Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

Special Issue Water and Human Settlements of the Future.-- 16 pages, 4 figures, 4 tables, supplementary materials https://www.mdpi.com/article/ 10.3390/w13192731/s1

Detalles Bibliográficos
Autores: Infante, Nury, Rodríguez, Refugio, Bartolo, Yaneth, Sánchez, Olga, Sanz-Sáez, Isabel, Bermeo, Lizeth, Morató, Jordi
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/251443
Acceso en línea:http://hdl.handle.net/10261/251443
Access Level:acceso abierto
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spelling Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coliInfante, NuryRodríguez, RefugioBartolo, YanethSánchez, OlgaSanz-Sáez, IsabelBermeo, LizethMorató, JordiSpecial Issue Water and Human Settlements of the Future.-- 16 pages, 4 figures, 4 tables, supplementary materials https://www.mdpi.com/article/ 10.3390/w13192731/s1Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27−4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growthThis research was funded by Mexico-CONACYT, grant number 3006060 and grant by the UNESCO Chair on Sustainability of the Polytechnic University of Catalonia (Spain)With the institutional support of the ‘Severo OchoaCentre of Excellence’ accreditation (CEX2019-000928-S).Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/251443reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/w13192731Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2514432026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
title Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
spellingShingle Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
Infante, Nury
title_short Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
title_full Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
title_fullStr Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
title_full_unstemmed Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
title_sort Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli
dc.creator.none.fl_str_mv Infante, Nury
Rodríguez, Refugio
Bartolo, Yaneth
Sánchez, Olga
Sanz-Sáez, Isabel
Bermeo, Lizeth
Morató, Jordi
author Infante, Nury
author_facet Infante, Nury
Rodríguez, Refugio
Bartolo, Yaneth
Sánchez, Olga
Sanz-Sáez, Isabel
Bermeo, Lizeth
Morató, Jordi
author_role author
author2 Rodríguez, Refugio
Bartolo, Yaneth
Sánchez, Olga
Sanz-Sáez, Isabel
Bermeo, Lizeth
Morató, Jordi
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Special Issue Water and Human Settlements of the Future.-- 16 pages, 4 figures, 4 tables, supplementary materials https://www.mdpi.com/article/ 10.3390/w13192731/s1
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/251443
url http://hdl.handle.net/10261/251443
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/w13192731
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415513906479104
score 15,811543

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