Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries

This article is part of the Research Topic: Advances in Oil Crops Research—Classical and New Approaches to Achieve Sustainable Productivity.-- Edited by: Dragana Miladinović, Johann Vollmann, Leire Molinero-Ruiz and Mariela Torres.

Detalles Bibliográficos
Autores: Zafra, Adoración, Carmona, Rosario, Traverso, José A., Hancock, John T., Goldman, Maria Helena S., Claros, M. Gonzalo, Hiscock, Simon J., Alché Ramírez, Juan de Dios
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164729
Acceso en línea:http://hdl.handle.net/10261/164729
Access Level:acceso abierto
Palabra clave:Pollen
Transcripts
Olive
Leaf
Gynoecium
SSH
Self-incompatibility
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oai_identifier_str oai:digital.csic.es:10261/164729
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
title Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
spellingShingle Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
Zafra, Adoración
Pollen
Transcripts
Olive
Leaf
Gynoecium
SSH
Self-incompatibility
title_short Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
title_full Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
title_fullStr Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
title_full_unstemmed Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
title_sort Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries
dc.creator.none.fl_str_mv Zafra, Adoración
Carmona, Rosario
Traverso, José A.
Hancock, John T.
Goldman, Maria Helena S.
Claros, M. Gonzalo
Hiscock, Simon J.
Alché Ramírez, Juan de Dios
author Zafra, Adoración
author_facet Zafra, Adoración
Carmona, Rosario
Traverso, José A.
Hancock, John T.
Goldman, Maria Helena S.
Claros, M. Gonzalo
Hiscock, Simon J.
Alché Ramírez, Juan de Dios
author_role author
author2 Carmona, Rosario
Traverso, José A.
Hancock, John T.
Goldman, Maria Helena S.
Claros, M. Gonzalo
Hiscock, Simon J.
Alché Ramírez, Juan de Dios
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Ministerio de Economía y Competitividad (España)
dc.subject.none.fl_str_mv Pollen
Transcripts
Olive
Leaf
Gynoecium
SSH
Self-incompatibility
topic Pollen
Transcripts
Olive
Leaf
Gynoecium
SSH
Self-incompatibility
description This article is part of the Research Topic: Advances in Oil Crops Research—Classical and New Approaches to Achieve Sustainable Productivity.-- Edited by: Dragana Miladinović, Johann Vollmann, Leire Molinero-Ruiz and Mariela Torres.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164729
url http://hdl.handle.net/10261/164729
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECO
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECO
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECO
Frontiers Research Topics
http://doi.org/10.3389/fpls.2017.01576
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive librariesZafra, AdoraciónCarmona, RosarioTraverso, José A.Hancock, John T.Goldman, Maria Helena S.Claros, M. GonzaloHiscock, Simon J.Alché Ramírez, Juan de DiosPollenTranscriptsOliveLeafGynoeciumSSHSelf-incompatibilityThis article is part of the Research Topic: Advances in Oil Crops Research—Classical and New Approaches to Achieve Sustainable Productivity.-- Edited by: Dragana Miladinović, Johann Vollmann, Leire Molinero-Ruiz and Mariela Torres.The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process, which determines the yield. Successful fertilization is mainly favored and sometimes needed of the presence of pollen grains from a different cultivar as the olive seizes a self-incompatibility system allegedly determined of the sporophytic type. The purpose of the present study was to identify key gene products involved in the function of olive pollen and pistil, in order to help elucidate the events and signaling processes, which happen during the courtship, pollen grain germination, and fertilization in olive. The use of subtractive SSH libraries constructed using, on the one hand one specific stage of the pistil development with germinating pollen grains, and on the other hand mature pollen grains may help to reveal the specific transcripts involved in the cited events. Such libraries have also been created by subtracting vegetative mRNAs (from leaves), in order to identify reproductive sequences only. A variety of transcripts have been identified in the mature pollen grains and in the pistil at the receptive stage. Among them, those related to defense, transport and oxidative metabolism are highlighted mainly in the pistil libraries where transcripts related to stress, and response to biotic and abiotic stimulus have a prominent position. Extensive lists containing information as regard to the specific transcripts determined for each stage and tissue are provided, as well as functional classifications of these gene products. Such lists were faced up to two recent datasets obtained in olive after transcriptomic and genomic approaches. The sequences and the differential expression level of the SSH-transcripts identified here, highly matched the transcriptomic information. Moreover, the unique presence of a representative number of these transcripts has been validated by means of qPCR approaches. The construction of SSH libraries using pistil and pollen, considering the high interaction between male-female counterparts, allowed the identification of transcripts with important roles in stigma physiology. The functions of many of the transcripts obtained are intimately related, and most of them are of pivotal importance in defense, pollen-stigma interaction and signaling.This work was supported by ERDF co-funded projects BFU2011-22779, BFU2016-77243-P, RTC-2015-4181-2 and RTC-2016-4824-2 (MINECO), P2011-CVI-7487 (Junta de Andalucía) and 201540E065 (CSIC). AZ thanks ceiA3 grant for Ph.D. in enterprises.Peer ReviewedFrontiers MediaJunta de AndalucíaMinisterio de Economía y Competitividad (España)2018201820172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/164729reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECOinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECOinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MINECOFrontiers Research Topicshttp://doi.org/10.3389/fpls.2017.01576info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1647292026-05-22T06:33:51Z
score 15,811543

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