Capillary electrophoresis analysis of prostate specific antigen (PSA)

The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its post-translational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reprodu...

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Bibliographic Details
Authors: Fariña Gómez, Noemi, Navarro-Calderon, Diana, Puerta, Angel de la, González, Mónica, Díez-Masa, José Carlos, Frutos, Mercedes de
Format: other
Status:Versión aceptada para publicación
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/200737
Online Access:http://hdl.handle.net/10261/200737
Access Level:Open access
Keyword:Reproducibility
Capillary conditioning
Prostate-specific antigen
PSA
Isoform
Proteoform
Glycoform
Cancer biomarkers
Description
Summary:The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its post-translational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reproducible CE methods are needed to detect them. In this chapter, a method for the analysis of prostate specific antigen (PSA) by Capillary Zone Electrophoresis (CZE) with UV detection is detailed. High reproducibility in the separation of a large number of PSA isoforms is achieved by performing capillary conditioning in acid media and by using a background electrolyte (BGE) at pH 8.0 formulated with decamethonium bromide and urea.

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