Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo

Nanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity are largely unknown and the Zebrafish embryo poses an ideal model to investigate them due to its high homolog...

ver descrição completa

Detalhes bibliográficos
Autores: Torres Ruiz, Mónica, Alba González, Mercedes de, González, M. Carmen, Cañas Portilla, Ana Isabel, Vieja, Antonio de la, Morales Camarzana, Consolación Mónica, Martín Folgar, Raquel
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Nacional de Educación a Distancia
Repositorio:e-spacio. Repositorio Institucional de la UNED
Idioma:inglés
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/12072
Acesso em linha:https://hdl.handle.net/20.500.14468/12072
Access Level:acceso abierto
Palavra-chave:Nanomaterial
Plastic
Development
Toxicity
Behavior
Hormones
id ES_a46a6b09f1500f6efdb82db5bd7a80dc
oai_identifier_str oai:e-spacio.uned.es:20.500.14468/12072
network_acronym_str ES
network_name_str España
repository_id_str
spelling Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryoTorres Ruiz, MónicaAlba González, Mercedes deGonzález, M. CarmenCañas Portilla, Ana IsabelVieja, Antonio de laMorales Camarzana, Consolación MónicaMartín Folgar, RaquelNanomaterialPlasticDevelopmentToxicityBehaviorHormonesNanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity are largely unknown and the Zebrafish embryo poses an ideal model to investigate them due to its high homology with humans. Our objective in the present study was to combine a battery of behavioral assays with the study of endocrine related gene expression, to further explore potential NP neurotoxic effects on animal behavior. Polystyrene nanoplastics (PSNP) were used to evaluate NP toxicity. Our neurobehavioral profiles include a tail coiling assay, a light/dark activity assay, two thigmotaxis anxiety assays (auditory and visual stimuli), and a startle response - habituation assay in response to auditory stimuli. Results show PSNP accumulated in eyes, neuromasts, brain, and digestive system organs. PSNP inhibited acetylcholinesterase and altered endocrine-related gene expression profiles both in the thyroid and glucocorticoid axes. At the whole organismlevel, we observed altered behaviors such as increased activity and anxiety at lower doses and lethargy at a higher dose, which could be due to a variety of complex mechanisms ranging from sensory organ and central nervous system effects to others such as hormonal imbalances. In addition, we present a hypothetical adverse outcome pathway related to these effects. In conclusion, this study provides new understanding into NP toxic effects on zebrafish embryo, emphasizing a critical role of endocrine disruption in observed neurotoxic behavioral effects, and improving our understanding of their potential health risks to human populations.Elseviere-Spacio UNED20242024-05-2020232023-05-2020232023-05-20journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14468/12072reponame:e-spacio. Repositorio Institucional de la UNEDinstname:Universidad Nacional de Educación a DistanciaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/deed.esoai:e-spacio.uned.es:20.500.14468/120722026-06-06T12:38:31Z
dc.title.none.fl_str_mv Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
title Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
spellingShingle Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
Torres Ruiz, Mónica
Nanomaterial
Plastic
Development
Toxicity
Behavior
Hormones
title_short Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
title_full Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
title_fullStr Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
title_full_unstemmed Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
title_sort Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
dc.creator.none.fl_str_mv Torres Ruiz, Mónica
Alba González, Mercedes de
González, M. Carmen
Cañas Portilla, Ana Isabel
Vieja, Antonio de la
Morales Camarzana, Consolación Mónica
Martín Folgar, Raquel
author Torres Ruiz, Mónica
author_facet Torres Ruiz, Mónica
Alba González, Mercedes de
González, M. Carmen
Cañas Portilla, Ana Isabel
Vieja, Antonio de la
Morales Camarzana, Consolación Mónica
Martín Folgar, Raquel
author_role author
author2 Alba González, Mercedes de
González, M. Carmen
Cañas Portilla, Ana Isabel
Vieja, Antonio de la
Morales Camarzana, Consolación Mónica
Martín Folgar, Raquel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv e-Spacio UNED
dc.subject.none.fl_str_mv Nanomaterial
Plastic
Development
Toxicity
Behavior
Hormones
topic Nanomaterial
Plastic
Development
Toxicity
Behavior
Hormones
description Nanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity are largely unknown and the Zebrafish embryo poses an ideal model to investigate them due to its high homology with humans. Our objective in the present study was to combine a battery of behavioral assays with the study of endocrine related gene expression, to further explore potential NP neurotoxic effects on animal behavior. Polystyrene nanoplastics (PSNP) were used to evaluate NP toxicity. Our neurobehavioral profiles include a tail coiling assay, a light/dark activity assay, two thigmotaxis anxiety assays (auditory and visual stimuli), and a startle response - habituation assay in response to auditory stimuli. Results show PSNP accumulated in eyes, neuromasts, brain, and digestive system organs. PSNP inhibited acetylcholinesterase and altered endocrine-related gene expression profiles both in the thyroid and glucocorticoid axes. At the whole organismlevel, we observed altered behaviors such as increased activity and anxiety at lower doses and lethargy at a higher dose, which could be due to a variety of complex mechanisms ranging from sensory organ and central nervous system effects to others such as hormonal imbalances. In addition, we present a hypothetical adverse outcome pathway related to these effects. In conclusion, this study provides new understanding into NP toxic effects on zebrafish embryo, emphasizing a critical role of endocrine disruption in observed neurotoxic behavioral effects, and improving our understanding of their potential health risks to human populations.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-05-20
2023
2023-05-20
2024
2024-05-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14468/12072
url https://hdl.handle.net/20.500.14468/12072
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/deed.es
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/deed.es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e-spacio. Repositorio Institucional de la UNED
instname:Universidad Nacional de Educación a Distancia
instname_str Universidad Nacional de Educación a Distancia
reponame_str e-spacio. Repositorio Institucional de la UNED
collection e-spacio. Repositorio Institucional de la UNED
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415495260700672
score 15,811543

Registos relacionados