Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress

High levels of atmospheric carbon dioxide result in suppression of plant photorespiration. The non-photorespiratory conditions (NPC) result in unbalancing the C/N metabolism, overproducing reactive oxygen species (ROS), and reducing stomatal activity. In plant stress responses, hydrogen sulfide (H2S...

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Autores: Luque, Carmen, García-Calderón, M., Gotor, Cecilia, Márquez, A. J., Aroca, Ángeles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/416355
Acceso en línea:http://hdl.handle.net/10261/416355
https://api.elsevier.com/content/abstract/scopus_id/105027641840
Access Level:acceso abierto
Palabra clave:Climate change
High CO₂
Hydrogen sulfide (H₂S)
Hypoxia
Oxidative stress
Photorespiration
Protein persulfidation
Stomatal regulation
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spelling Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stressLuque, CarmenGarcía-Calderón, M.Gotor, CeciliaMárquez, A. J.Aroca, ÁngelesClimate changeHigh CO₂Hydrogen sulfide (H₂S)HypoxiaOxidative stressPhotorespirationProtein persulfidationStomatal regulationHigh levels of atmospheric carbon dioxide result in suppression of plant photorespiration. The non-photorespiratory conditions (NPC) result in unbalancing the C/N metabolism, overproducing reactive oxygen species (ROS), and reducing stomatal activity. In plant stress responses, hydrogen sulfide (H2S) has been identified as an important signaling molecule through persulfidation of specific proteins. Previous works demonstrated that H₂S protects Arabidopsis thaliana against NPC-induced stress, and this work investigates the molecular basis of such protection. H₂S modulates a metabolic reprogramming influencing elemental homeostasis of C/N ratio, amino acids profile, central carbon metabolites and accumulation of polyunsaturated fatty acids (PUFAs). Persulfidation level under NPC was also restored after H₂S treatment. At the transcriptomic level, several well-known hypoxia marker genes, such as plant CYSTEINE OXIDASE 1 and 2, ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF71 AND ETHYLENE RECEPTOR 2, are induced under NPC, and sulfide treatment decreases their expression levels to the ones in active photorespiration conditions (APC). H₂S also negatively regulates ABA signaling by targeting genes controlling ion transport and stomatal development which are involved in stomatal function. These integrated responses across metabolism, redox regulation and developmental programming emphasize the key contribution of H₂S to orchestrating plant adaptation to high CO₂ environments, positioning it as a master regulator that ensures plant resilience in the face of climate change.This work was supported by the ERDF ‘A way of making Europe’ and MCIN/AEI/10.13039/501100011033 (grant no PID2022-141885NB-I00 and PID2021-122353OB-100); Junta de Andalucía (grant no PROYEXCEL_00177).Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionJunta de AndalucíaLuque, Carmen [0009-0001-6080-7100]García-Calderón, M. [0000-0003-3595-3963]Gotor, Cecilia [0000-0003-4272-7446]Márquez, A. J. [0000-0001-7254-1496]Aroca, Ángeles [0000-0003-4915-170X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/416355https://api.elsevier.com/content/abstract/scopus_id/105027641840reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141885NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122353OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.stress.2026.101222https://doi.org/10.1016/j.stress.2026.101222Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4163552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
title Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
spellingShingle Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
Luque, Carmen
Climate change
High CO₂
Hydrogen sulfide (H₂S)
Hypoxia
Oxidative stress
Photorespiration
Protein persulfidation
Stomatal regulation
title_short Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
title_full Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
title_fullStr Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
title_full_unstemmed Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
title_sort Hydrogen sulfide improves performance under suppressed photorespiration in Arabidopsis thaliana and orchestrates molecular reprogramming to alleviate stress
dc.creator.none.fl_str_mv Luque, Carmen
García-Calderón, M.
Gotor, Cecilia
Márquez, A. J.
Aroca, Ángeles
author Luque, Carmen
author_facet Luque, Carmen
García-Calderón, M.
Gotor, Cecilia
Márquez, A. J.
Aroca, Ángeles
author_role author
author2 García-Calderón, M.
Gotor, Cecilia
Márquez, A. J.
Aroca, Ángeles
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Junta de Andalucía
Luque, Carmen [0009-0001-6080-7100]
García-Calderón, M. [0000-0003-3595-3963]
Gotor, Cecilia [0000-0003-4272-7446]
Márquez, A. J. [0000-0001-7254-1496]
Aroca, Ángeles [0000-0003-4915-170X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Climate change
High CO₂
Hydrogen sulfide (H₂S)
Hypoxia
Oxidative stress
Photorespiration
Protein persulfidation
Stomatal regulation
topic Climate change
High CO₂
Hydrogen sulfide (H₂S)
Hypoxia
Oxidative stress
Photorespiration
Protein persulfidation
Stomatal regulation
description High levels of atmospheric carbon dioxide result in suppression of plant photorespiration. The non-photorespiratory conditions (NPC) result in unbalancing the C/N metabolism, overproducing reactive oxygen species (ROS), and reducing stomatal activity. In plant stress responses, hydrogen sulfide (H2S) has been identified as an important signaling molecule through persulfidation of specific proteins. Previous works demonstrated that H₂S protects Arabidopsis thaliana against NPC-induced stress, and this work investigates the molecular basis of such protection. H₂S modulates a metabolic reprogramming influencing elemental homeostasis of C/N ratio, amino acids profile, central carbon metabolites and accumulation of polyunsaturated fatty acids (PUFAs). Persulfidation level under NPC was also restored after H₂S treatment. At the transcriptomic level, several well-known hypoxia marker genes, such as plant CYSTEINE OXIDASE 1 and 2, ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF71 AND ETHYLENE RECEPTOR 2, are induced under NPC, and sulfide treatment decreases their expression levels to the ones in active photorespiration conditions (APC). H₂S also negatively regulates ABA signaling by targeting genes controlling ion transport and stomatal development which are involved in stomatal function. These integrated responses across metabolism, redox regulation and developmental programming emphasize the key contribution of H₂S to orchestrating plant adaptation to high CO₂ environments, positioning it as a master regulator that ensures plant resilience in the face of climate change.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/416355
https://api.elsevier.com/content/abstract/scopus_id/105027641840
url http://hdl.handle.net/10261/416355
https://api.elsevier.com/content/abstract/scopus_id/105027641840
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141885NB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122353OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.stress.2026.101222
https://doi.org/10.1016/j.stress.2026.101222
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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