Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA
[EN] Abscisic acid (ABA) is an essential hormone for plant development and stress responses. ABA signaling is suppressed by clade A PP2C phosphatases, which function as key repressors of this pathway through inhibiting ABA-activated SnRK2s (SNF1-related protein kinases). Upon ABA perception, the PYR...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/82486 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/82486 |
| Access Level: | acceso abierto |
| Palabra clave: | SNRK2 protein-kinases Thaliana L Heynh Arabidopsis thaliana Differential expression ABA 2C Transduction Degradation Receptor Stress BIOQUIMICA Y BIOLOGIA MOLECULAR |
| id |
ES_a4258a4f48b0af3feda7bfefca642dcc |
|---|---|
| oai_identifier_str |
oai:riunet.upv.es:10251/82486 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CAWu, QianZhang, XuPeirats-Llobet, MartaWang, XiaofengCui, ShaoYu, XiangchunAn, ChengcaiBelda Palazón, BorjaRodríguez Egea, Pedro Luís|||0000-0002-5886-9425SNRK2 protein-kinasesThaliana L HeynhArabidopsis thalianaDifferential expressionABA2CTransductionDegradationReceptorStressBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Abscisic acid (ABA) is an essential hormone for plant development and stress responses. ABA signaling is suppressed by clade A PP2C phosphatases, which function as key repressors of this pathway through inhibiting ABA-activated SnRK2s (SNF1-related protein kinases). Upon ABA perception, the PYR/PYL/RCAR ABA receptors bind to PP2Cs with high affinity and biochemically inhibit their activity. While thismechanismhas been extensively studied, how PP2Cs are regulated at the protein level is only starting to be explored. Arabidopsis thaliana RING DOMAIN LIGASE5 (RGLG5) belongs to a five-member E3 ubiquitin ligase family whose target proteins remain unknown. We report that RGLG5, together with RGLG1, releases the PP2C blockade of ABA signaling by mediating PP2CA protein degradation. ABA promotes the interaction of PP2CA with both E3 ligases, which mediate ubiquitination of PP2CA and are required for ABA-dependent PP2CA turnover. Downregulation of RGLG1 and RGLG5 stabilizes endogenous PP2CA and diminishes ABA-mediated responses. Moreover, the reduced response to ABA in germination assays is suppressed in the rglg1 amiR (artificial microRNA)-rglg5 pp2ca-1 triple mutant, supporting a functional link among these loci. Overall, our data indicate that RGLG1 and RGLG5 are important modulators of ABA signaling, and they unveil amechanismfor activation of the ABA pathway by controlling PP2C half-life.We thank Andreas Bachmair for the rglg1 mutant, Sean R. Cutler for the pyr1 pyl1 pyl2 pyl4 seeds, Dapeng Zhang for the transgenic material harboring ABI2, Hongwei Guo and Jianmin Zhou for the pCAMBIA1300-Nluc and pCAMBIA1300-Cluc vectors, and John Olson for assistance in English editing. Work in C.A.'s laboratory was supported by grants from the National Key Basic Science "973" Program (Grant 2012CB114006), the National Natural Science Foundation (Grants 31272023, 31170231, and 90817001) of the Chinese government, and by the State Key Laboratory of Protein and Plant Gene Research, Peking University. Work in P.L.R.'s laboratory was supported by Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (Grant BIO2014-52537-R).American Society of Plant BiologistsInstituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Economía y CompetitividadNational Key Research and Development Program of ChinaRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/82486reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-52537-R REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIAopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/824862026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| title |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| spellingShingle |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA Wu, Qian SNRK2 protein-kinases Thaliana L Heynh Arabidopsis thaliana Differential expression ABA 2C Transduction Degradation Receptor Stress BIOQUIMICA Y BIOLOGIA MOLECULAR |
| title_short |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| title_full |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| title_fullStr |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| title_full_unstemmed |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| title_sort |
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA |
| dc.creator.none.fl_str_mv |
Wu, Qian Zhang, Xu Peirats-Llobet, Marta Wang, Xiaofeng Cui, Shao Yu, Xiangchun An, Chengcai Belda Palazón, Borja Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 |
| author |
Wu, Qian |
| author_facet |
Wu, Qian Zhang, Xu Peirats-Llobet, Marta Wang, Xiaofeng Cui, Shao Yu, Xiangchun An, Chengcai Belda Palazón, Borja Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 |
| author_role |
author |
| author2 |
Zhang, Xu Peirats-Llobet, Marta Wang, Xiaofeng Cui, Shao Yu, Xiangchun An, Chengcai Belda Palazón, Borja Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto Universitario Mixto de Biología Molecular y Celular de Plantas Ministerio de Economía y Competitividad National Key Research and Development Program of China Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
SNRK2 protein-kinases Thaliana L Heynh Arabidopsis thaliana Differential expression ABA 2C Transduction Degradation Receptor Stress BIOQUIMICA Y BIOLOGIA MOLECULAR |
| topic |
SNRK2 protein-kinases Thaliana L Heynh Arabidopsis thaliana Differential expression ABA 2C Transduction Degradation Receptor Stress BIOQUIMICA Y BIOLOGIA MOLECULAR |
| description |
[EN] Abscisic acid (ABA) is an essential hormone for plant development and stress responses. ABA signaling is suppressed by clade A PP2C phosphatases, which function as key repressors of this pathway through inhibiting ABA-activated SnRK2s (SNF1-related protein kinases). Upon ABA perception, the PYR/PYL/RCAR ABA receptors bind to PP2Cs with high affinity and biochemically inhibit their activity. While thismechanismhas been extensively studied, how PP2Cs are regulated at the protein level is only starting to be explored. Arabidopsis thaliana RING DOMAIN LIGASE5 (RGLG5) belongs to a five-member E3 ubiquitin ligase family whose target proteins remain unknown. We report that RGLG5, together with RGLG1, releases the PP2C blockade of ABA signaling by mediating PP2CA protein degradation. ABA promotes the interaction of PP2CA with both E3 ligases, which mediate ubiquitination of PP2CA and are required for ABA-dependent PP2CA turnover. Downregulation of RGLG1 and RGLG5 stabilizes endogenous PP2CA and diminishes ABA-mediated responses. Moreover, the reduced response to ABA in germination assays is suppressed in the rglg1 amiR (artificial microRNA)-rglg5 pp2ca-1 triple mutant, supporting a functional link among these loci. Overall, our data indicate that RGLG1 and RGLG5 are important modulators of ABA signaling, and they unveil amechanismfor activation of the ABA pathway by controlling PP2C half-life. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-09-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/82486 |
| url |
https://riunet.upv.es/handle/10251/82486 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-52537-R REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIA |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
American Society of Plant Biologists |
| publisher.none.fl_str_mv |
American Society of Plant Biologists |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869415473661083648 |
| score |
15,300719 |