Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods

One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Stan...

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Authors: Segura Montero, Verónica, Díaz, Jacobo, Ruiz Carnicer, Ángela, Muñoz Suano, Alba, Carrillo Carrión, Carolina, Sousa Martín, Carolina, Cebolla, Ángel, Comino Montilla, Isabel María
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/108127
Online Access:https://hdl.handle.net/11441/108127
https://doi.org/10.3390/foods10030652
Access Level:Open access
Keyword:Celiac disease
Gluten extraction solution
Gluten proteins
Gluten-free diet
id ES_a3fe2c83ae8b79597e003af37483bf1c
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network_name_str España
repository_id_str
spelling Rapid, effective, and versatile extraction of gluten in food with application on different immunological methodsSegura Montero, VerónicaDíaz, JacoboRuiz Carnicer, ÁngelaMuñoz Suano, AlbaCarrillo Carrión, CarolinaSousa Martín, CarolinaCebolla, ÁngelComino Montilla, Isabel MaríaCeliac diseaseGluten extraction solutionGluten proteinsGluten-free dietOne of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA.Ministerio de Economía y Competitividad RTC-2016-5441-1Premio Mensual Publicación Científica Destacada de la US. Facultad de FarmaciaMultidisciplinary Digital Publishing Institute (MDPI)Microbiología y Parasitología2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/108127https://doi.org/10.3390/foods10030652reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFoods, 10 (3), 652.RTC-2016-5441-1https://doi.org/10.3390/foods10030652info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1081272026-06-17T12:51:07Z
dc.title.none.fl_str_mv Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
title Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
spellingShingle Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
Segura Montero, Verónica
Celiac disease
Gluten extraction solution
Gluten proteins
Gluten-free diet
title_short Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
title_full Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
title_fullStr Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
title_full_unstemmed Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
title_sort Rapid, effective, and versatile extraction of gluten in food with application on different immunological methods
dc.creator.none.fl_str_mv Segura Montero, Verónica
Díaz, Jacobo
Ruiz Carnicer, Ángela
Muñoz Suano, Alba
Carrillo Carrión, Carolina
Sousa Martín, Carolina
Cebolla, Ángel
Comino Montilla, Isabel María
author Segura Montero, Verónica
author_facet Segura Montero, Verónica
Díaz, Jacobo
Ruiz Carnicer, Ángela
Muñoz Suano, Alba
Carrillo Carrión, Carolina
Sousa Martín, Carolina
Cebolla, Ángel
Comino Montilla, Isabel María
author_role author
author2 Díaz, Jacobo
Ruiz Carnicer, Ángela
Muñoz Suano, Alba
Carrillo Carrión, Carolina
Sousa Martín, Carolina
Cebolla, Ángel
Comino Montilla, Isabel María
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
dc.subject.none.fl_str_mv Celiac disease
Gluten extraction solution
Gluten proteins
Gluten-free diet
topic Celiac disease
Gluten extraction solution
Gluten proteins
Gluten-free diet
description One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/108127
https://doi.org/10.3390/foods10030652
url https://hdl.handle.net/11441/108127
https://doi.org/10.3390/foods10030652
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Foods, 10 (3), 652.
RTC-2016-5441-1
https://doi.org/10.3390/foods10030652
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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