Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard

A growing number of weed biotypes showing resistance to acetolactate synthase (ALS)-inhibitors have been reported in several species, notably including Sinapis arvensis L. Two putative resistant (R) populations of S. arvensis from Tunisia were subjected to greenhouse and laboratory investigations to...

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Authors: Chtourou, Myriem, Osuna, María D., Vázquez-García, José G., De Prado, Rafael, Lozano-Juste, Jorge, Mora Marin, German, Hada, Zeineb, Souissi, Thouraya, Torra Farré, Joel
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/468078
Online Access:https://doi.org/10.1016/j.plaphy.2025.110043
https://hdl.handle.net/10459.1/468078
Access Level:Open access
Keyword:ALS inhibitor
Broad-resistance
Cytochrome P450 monooxygenases
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spelling Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustardChtourou, MyriemOsuna, María D.Vázquez-García, José G.De Prado, RafaelLozano-Juste, JorgeMora Marin, GermanHada, ZeinebSouissi, ThourayaTorra Farré, JoelALS inhibitorBroad-resistanceCytochrome P450 monooxygenasesA growing number of weed biotypes showing resistance to acetolactate synthase (ALS)-inhibitors have been reported in several species, notably including Sinapis arvensis L. Two putative resistant (R) populations of S. arvensis from Tunisia were subjected to greenhouse and laboratory investigations to validate resistance to ALS-inhibitors and to determinate the mechanisms involved. The results indicated that both populations were resistant to four distinct ALS-inhibiting herbicides, tribenuron-methyl (TM), florasulam, flucarbazone and imazamox (IMZ), thereby confirming cross-resistance between them. The dose of (TM) required to achieve a 50 % reduction in plant growth (ED50) and 50 % mortality (LC50) in R populations of S. arvensis was found to be at least 60 times greater than the recommended field dose (18.7 g ai ha−1) applied in cereal crops in Tunisia, indicating a significantly elevated resistance factor. Synergist experiments using malathion as a cytochrome P450 (Cyt-P450) inhibitor demonstrated a reduction in resistance to imazamox (IMZ) in both resistant (R) biotypes, indicating that Cyt-P450 plays a partial role in the resistance mechanism. In addition, ALS gene analysis identified three key point mutations, Pro197Ala, Asp376Glu and Trp574Leu, in both R populations. The docking analysis demonstrated that Asp376Glu mutation in S. arvensis could confer cross-resistance to IMZ and TM herbicides. CAPS and dCAPS methods were developed for detecting the Trp574Leu and Asp376Glu mutations, respectively, in S arvensis and it was shown that work efficiently. Fortunately, the study also confirmed that 2,4-D still effectively controlled S. arvensis populations. This study provides valuable insights into the mechanisms underlying herbicide resistance in S.arvensis populations from Tunisia, demonstrating that both target-site resistance (TSR) and non-target-site resistance (NTSR) contribute to the species' broad-spectrum resistance against four dissimilar ALS-inhibitors.Joel Torra acknowledges support from the Spanish Ministry of Science, Innovation, and Universities (grant Ramon y Cajal RYC2018- 023866-I) and by the Spanish State Research Agency, Spain (AEI) and the European Regional Development Fund, EU (ERDF) through the projects AGL2017-83325-C4-2-R and PID2020-113229RB-C42. Work at the Chemical Biology Group of the IBMCP (JL-J) is funded by Spanish MCIN/AEI/PID2021-128826OA-I00, RYC2020-029097-I, Plan GenT (CISEJI/2022/26) grants and the AGROALNEXT program supported by MCIN with funding from European Union NextGenerationEU (PRTRC17.I1) and by Generalitat Valenciana.Elsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.plaphy.2025.110043https://hdl.handle.net/10459.1/468078reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-RReproducció del document publicat a https://doi.org/10.1016/j.plaphy.2025.110043Plant Physiology and Biochemistry, 2025, vol. 225, 110043cc-by (c) Myriem Chtourou et al., 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/4680782026-05-29T05:05:01Z
dc.title.none.fl_str_mv Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
title Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
spellingShingle Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
Chtourou, Myriem
ALS inhibitor
Broad-resistance
Cytochrome P450 monooxygenases
title_short Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
title_full Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
title_fullStr Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
title_full_unstemmed Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
title_sort Several point mutations and metabolism confer cross-resistance to ALS-inhibiting herbicides in Tunisian wild mustard
dc.creator.none.fl_str_mv Chtourou, Myriem
Osuna, María D.
Vázquez-García, José G.
De Prado, Rafael
Lozano-Juste, Jorge
Mora Marin, German
Hada, Zeineb
Souissi, Thouraya
Torra Farré, Joel
author Chtourou, Myriem
author_facet Chtourou, Myriem
Osuna, María D.
Vázquez-García, José G.
De Prado, Rafael
Lozano-Juste, Jorge
Mora Marin, German
Hada, Zeineb
Souissi, Thouraya
Torra Farré, Joel
author_role author
author2 Osuna, María D.
Vázquez-García, José G.
De Prado, Rafael
Lozano-Juste, Jorge
Mora Marin, German
Hada, Zeineb
Souissi, Thouraya
Torra Farré, Joel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALS inhibitor
Broad-resistance
Cytochrome P450 monooxygenases
topic ALS inhibitor
Broad-resistance
Cytochrome P450 monooxygenases
description A growing number of weed biotypes showing resistance to acetolactate synthase (ALS)-inhibitors have been reported in several species, notably including Sinapis arvensis L. Two putative resistant (R) populations of S. arvensis from Tunisia were subjected to greenhouse and laboratory investigations to validate resistance to ALS-inhibitors and to determinate the mechanisms involved. The results indicated that both populations were resistant to four distinct ALS-inhibiting herbicides, tribenuron-methyl (TM), florasulam, flucarbazone and imazamox (IMZ), thereby confirming cross-resistance between them. The dose of (TM) required to achieve a 50 % reduction in plant growth (ED50) and 50 % mortality (LC50) in R populations of S. arvensis was found to be at least 60 times greater than the recommended field dose (18.7 g ai ha−1) applied in cereal crops in Tunisia, indicating a significantly elevated resistance factor. Synergist experiments using malathion as a cytochrome P450 (Cyt-P450) inhibitor demonstrated a reduction in resistance to imazamox (IMZ) in both resistant (R) biotypes, indicating that Cyt-P450 plays a partial role in the resistance mechanism. In addition, ALS gene analysis identified three key point mutations, Pro197Ala, Asp376Glu and Trp574Leu, in both R populations. The docking analysis demonstrated that Asp376Glu mutation in S. arvensis could confer cross-resistance to IMZ and TM herbicides. CAPS and dCAPS methods were developed for detecting the Trp574Leu and Asp376Glu mutations, respectively, in S arvensis and it was shown that work efficiently. Fortunately, the study also confirmed that 2,4-D still effectively controlled S. arvensis populations. This study provides valuable insights into the mechanisms underlying herbicide resistance in S.arvensis populations from Tunisia, demonstrating that both target-site resistance (TSR) and non-target-site resistance (NTSR) contribute to the species' broad-spectrum resistance against four dissimilar ALS-inhibitors.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.plaphy.2025.110043
https://hdl.handle.net/10459.1/468078
url https://doi.org/10.1016/j.plaphy.2025.110043
https://hdl.handle.net/10459.1/468078
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-R
Reproducció del document publicat a https://doi.org/10.1016/j.plaphy.2025.110043
Plant Physiology and Biochemistry, 2025, vol. 225, 110043
dc.rights.none.fl_str_mv cc-by (c) Myriem Chtourou et al., 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Myriem Chtourou et al., 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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