Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens

In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples wer...

ver descrição completa

Detalhes bibliográficos
Autores: Chaves Hernández, Aida Jeannette|||0000-0002-2856-7418, Busquets, Núria|||0000-0001-5246-8260, Valle, Rosa, Rivas, Raquel, Vergara-Alert, Júlia|||0000-0001-7484-444X, Dolz, Roser, Ramis Salva, Antonio José|||0000-0001-6755-8066, Darji, Ayub|||0000-0002-0967-3303, Majó i Masferrer, Natàlia|||0000-0003-0189-9751
Formato: artículo
Fecha de publicación:2011
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:22864
Acesso em linha:https://ddd.uab.cat/record/22864
https://dx.doi.org/urn:doi:10.1186/1297-9716-42-106
Access Level:acceso abierto
Palavra-chave:Grip aviària
Avian influenza
Sistema nerviós
Nervous system
id ES_a3db385e7fc72b938392c53d1d7f5621
oai_identifier_str oai:ddd.uab.cat:22864
network_acronym_str ES
network_name_str España
repository_id_str
spelling Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickensChaves Hernández, Aida Jeannette|||0000-0002-2856-7418Busquets, Núria|||0000-0001-5246-8260Valle, RosaRivas, RaquelVergara-Alert, Júlia|||0000-0001-7484-444XDolz, RoserRamis Salva, Antonio José|||0000-0001-6755-8066Darji, Ayub|||0000-0002-0967-3303Majó i Masferrer, Natàlia|||0000-0003-0189-9751Grip aviàriaAvian influenzaSistema nerviósNervous systemIn order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days post-inoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Siaα2,3 Gal and Siaα2,6 Gal receptors in brain vascular endothelial cells, and Siaα2,3 Gal receptors in ependymal and choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the haematogenous route, with early and generalized spreading through the CSF. 22011-01-0120112011-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/22864https://dx.doi.org/urn:doi:10.1186/1297-9716-42-106reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:228642026-06-06T12:50:31Z
dc.title.none.fl_str_mv Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
title Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
spellingShingle Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
Chaves Hernández, Aida Jeannette|||0000-0002-2856-7418
Grip aviària
Avian influenza
Sistema nerviós
Nervous system
title_short Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
title_full Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
title_fullStr Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
title_full_unstemmed Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
title_sort Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
dc.creator.none.fl_str_mv Chaves Hernández, Aida Jeannette|||0000-0002-2856-7418
Busquets, Núria|||0000-0001-5246-8260
Valle, Rosa
Rivas, Raquel
Vergara-Alert, Júlia|||0000-0001-7484-444X
Dolz, Roser
Ramis Salva, Antonio José|||0000-0001-6755-8066
Darji, Ayub|||0000-0002-0967-3303
Majó i Masferrer, Natàlia|||0000-0003-0189-9751
author Chaves Hernández, Aida Jeannette|||0000-0002-2856-7418
author_facet Chaves Hernández, Aida Jeannette|||0000-0002-2856-7418
Busquets, Núria|||0000-0001-5246-8260
Valle, Rosa
Rivas, Raquel
Vergara-Alert, Júlia|||0000-0001-7484-444X
Dolz, Roser
Ramis Salva, Antonio José|||0000-0001-6755-8066
Darji, Ayub|||0000-0002-0967-3303
Majó i Masferrer, Natàlia|||0000-0003-0189-9751
author_role author
author2 Busquets, Núria|||0000-0001-5246-8260
Valle, Rosa
Rivas, Raquel
Vergara-Alert, Júlia|||0000-0001-7484-444X
Dolz, Roser
Ramis Salva, Antonio José|||0000-0001-6755-8066
Darji, Ayub|||0000-0002-0967-3303
Majó i Masferrer, Natàlia|||0000-0003-0189-9751
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Grip aviària
Avian influenza
Sistema nerviós
Nervous system
topic Grip aviària
Avian influenza
Sistema nerviós
Nervous system
description In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days post-inoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Siaα2,3 Gal and Siaα2,6 Gal receptors in brain vascular endothelial cells, and Siaα2,3 Gal receptors in ependymal and choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the haematogenous route, with early and generalized spreading through the CSF.
publishDate 2011
dc.date.none.fl_str_mv 2
2011-01-01
2011
2011-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/22864
https://dx.doi.org/urn:doi:10.1186/1297-9716-42-106
url https://ddd.uab.cat/record/22864
https://dx.doi.org/urn:doi:10.1186/1297-9716-42-106
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415432102871040
score 15.301603

Registos relacionados