Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda

In rural parts of Africa, dogs live in close association with humans and livestock, roam freely, and usually do not receive prophylactic measures. Thus, they are a source of infectious disease for humans and for wildlife such as protected carnivores. In 2011, an epidemiological study was carried out...

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Authors: Proboste, Tatiana, Kalema-Zikusoka, Gladys|||0000-0003-3473-3205, Altet Sanahujes, Laura, Solano Gallego, Laia|||0000-0001-8479-4896, Fernández de Mera, Isabel G., Chirife, Andrea D., Muro, Jesús, Bach i Raich, Ester, Piazza, Antonio, Cevidanes, Aitor, Blanda, Valeria, Mugisha, Lawrence, de la Fuente, José, Caracappa, Santo, Millán, Javier
Format: article
Publication Date:2015
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:254381
Online Access:https://ddd.uab.cat/record/254381
https://dx.doi.org/urn:doi:10.1186/s13071-015-0919-x
Access Level:Open access
Keyword:Babesia
Dogs
East Africa
Ehrlichia
Rickettsia
Tick-borne pathogens
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spelling Infection and exposure to vector-borne pathogens in rural dogs and their ticks, UgandaProboste, TatianaKalema-Zikusoka, Gladys|||0000-0003-3473-3205Altet Sanahujes, LauraSolano Gallego, Laia|||0000-0001-8479-4896Fernández de Mera, Isabel G.Chirife, Andrea D.Muro, JesúsBach i Raich, EsterPiazza, AntonioCevidanes, AitorBlanda, ValeriaMugisha, Lawrencede la Fuente, JoséCaracappa, SantoMillán, JavierBabesiaDogsEast AfricaEhrlichiaRickettsiaTick-borne pathogensIn rural parts of Africa, dogs live in close association with humans and livestock, roam freely, and usually do not receive prophylactic measures. Thus, they are a source of infectious disease for humans and for wildlife such as protected carnivores. In 2011, an epidemiological study was carried out around three conservation areas in Uganda to detect the presence and determine the prevalence of vector-borne pathogens in rural dogs and associated ticks to evaluate the risk that these pathogens pose to humans and wildlife. Serum samples (n = 105), blood smears (n = 43) and blood preserved on FTA cards (n = 38) and ticks (58 monospecific pools of Haemaphysalis leachi and Rhipicephalus praetextatus including 312 ticks from 52 dogs) were collected from dogs. Dog sera were tested by indirect immunofluorescence to detect the presence of antibodies against Rickettsia conorii and Ehrlichia canis. Antibodies against R. conorii were also examined by indirect enzyme immunoassay. Real time PCR for the detection of Rickettsia spp., Anaplasmataceae, Bartonella spp. and Babesia spp. was performed in DNA extracted from FTA cards and ticks. 99 % of the dogs were seropositive to Rickettsia spp. and 29.5 % to Ehrlichia spp. Molecular analyses revealed that 7.8 % of the blood samples were infected with Babesia rossi, and all were negative for Rickettsia spp. and Ehrlichia spp. Ticks were infected with Rickettsia sp. (18.9 %), including R. conorii and R. massiliae ; Ehrlichia sp. (18.9 %), including E. chaffeensis and Anaplasma platys ; and B. rossi (1.7 %). Bartonella spp. was not detected in any of the blood or tick samples. This study confirms the presence of previously undetected vector-borne pathogens of humans and animals in East Africa. We recommend that dog owners in rural Uganda be advised to protect their animals against ectoparasites to prevent the transmission of pathogens to humans and wildlife. 22015-01-0120152015-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/254381https://dx.doi.org/urn:doi:10.1186/s13071-015-0919-xreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2543812026-06-06T12:50:31Z
dc.title.none.fl_str_mv Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
title Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
spellingShingle Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
Proboste, Tatiana
Babesia
Dogs
East Africa
Ehrlichia
Rickettsia
Tick-borne pathogens
title_short Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
title_full Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
title_fullStr Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
title_full_unstemmed Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
title_sort Infection and exposure to vector-borne pathogens in rural dogs and their ticks, Uganda
dc.creator.none.fl_str_mv Proboste, Tatiana
Kalema-Zikusoka, Gladys|||0000-0003-3473-3205
Altet Sanahujes, Laura
Solano Gallego, Laia|||0000-0001-8479-4896
Fernández de Mera, Isabel G.
Chirife, Andrea D.
Muro, Jesús
Bach i Raich, Ester
Piazza, Antonio
Cevidanes, Aitor
Blanda, Valeria
Mugisha, Lawrence
de la Fuente, José
Caracappa, Santo
Millán, Javier
author Proboste, Tatiana
author_facet Proboste, Tatiana
Kalema-Zikusoka, Gladys|||0000-0003-3473-3205
Altet Sanahujes, Laura
Solano Gallego, Laia|||0000-0001-8479-4896
Fernández de Mera, Isabel G.
Chirife, Andrea D.
Muro, Jesús
Bach i Raich, Ester
Piazza, Antonio
Cevidanes, Aitor
Blanda, Valeria
Mugisha, Lawrence
de la Fuente, José
Caracappa, Santo
Millán, Javier
author_role author
author2 Kalema-Zikusoka, Gladys|||0000-0003-3473-3205
Altet Sanahujes, Laura
Solano Gallego, Laia|||0000-0001-8479-4896
Fernández de Mera, Isabel G.
Chirife, Andrea D.
Muro, Jesús
Bach i Raich, Ester
Piazza, Antonio
Cevidanes, Aitor
Blanda, Valeria
Mugisha, Lawrence
de la Fuente, José
Caracappa, Santo
Millán, Javier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia
Dogs
East Africa
Ehrlichia
Rickettsia
Tick-borne pathogens
topic Babesia
Dogs
East Africa
Ehrlichia
Rickettsia
Tick-borne pathogens
description In rural parts of Africa, dogs live in close association with humans and livestock, roam freely, and usually do not receive prophylactic measures. Thus, they are a source of infectious disease for humans and for wildlife such as protected carnivores. In 2011, an epidemiological study was carried out around three conservation areas in Uganda to detect the presence and determine the prevalence of vector-borne pathogens in rural dogs and associated ticks to evaluate the risk that these pathogens pose to humans and wildlife. Serum samples (n = 105), blood smears (n = 43) and blood preserved on FTA cards (n = 38) and ticks (58 monospecific pools of Haemaphysalis leachi and Rhipicephalus praetextatus including 312 ticks from 52 dogs) were collected from dogs. Dog sera were tested by indirect immunofluorescence to detect the presence of antibodies against Rickettsia conorii and Ehrlichia canis. Antibodies against R. conorii were also examined by indirect enzyme immunoassay. Real time PCR for the detection of Rickettsia spp., Anaplasmataceae, Bartonella spp. and Babesia spp. was performed in DNA extracted from FTA cards and ticks. 99 % of the dogs were seropositive to Rickettsia spp. and 29.5 % to Ehrlichia spp. Molecular analyses revealed that 7.8 % of the blood samples were infected with Babesia rossi, and all were negative for Rickettsia spp. and Ehrlichia spp. Ticks were infected with Rickettsia sp. (18.9 %), including R. conorii and R. massiliae ; Ehrlichia sp. (18.9 %), including E. chaffeensis and Anaplasma platys ; and B. rossi (1.7 %). Bartonella spp. was not detected in any of the blood or tick samples. This study confirms the presence of previously undetected vector-borne pathogens of humans and animals in East Africa. We recommend that dog owners in rural Uganda be advised to protect their animals against ectoparasites to prevent the transmission of pathogens to humans and wildlife.
publishDate 2015
dc.date.none.fl_str_mv 2
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/254381
https://dx.doi.org/urn:doi:10.1186/s13071-015-0919-x
url https://ddd.uab.cat/record/254381
https://dx.doi.org/urn:doi:10.1186/s13071-015-0919-x
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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