New synthetic inhibitors of fatty acid synthase with anitcancer activity

Fatty acid synthase (FASN) is a lipogenic enzyme that is highly expressed in different human cancers. Here we report the development of a new series of polyphenolic compounds 5-30 that have been evaluated for their cytotoxic capacity in SK-Br3 cells, a human breast cancer cell line with high FASN ex...

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Detalles Bibliográficos
Autores: Turrado, Carlos, Puig i Miquel, Teresa, García-Cárceles, Javier, Artola, Marta, Benhamú, Bellinda, Ortega Gutiérrez, Sílvia, Relat, Joana, Oliveras, Glòria, Blancafort Jorquera, Adriana, Haro, Diego, Marrero, Pedro F., Colomer, Ramon, López-Rodríguez, María L.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/26126
Acceso en línea:http://hdl.handle.net/10256/26126
Access Level:acceso abierto
Palabra clave:Inhibidors enzimàtics
Enzyme inhibitors
Càncer -- Tractament
Cancer -- Treatment
Mama -- Càncer
Breast -- Cancer
Descripción
Sumario:Fatty acid synthase (FASN) is a lipogenic enzyme that is highly expressed in different human cancers. Here we report the development of a new series of polyphenolic compounds 5-30 that have been evaluated for their cytotoxic capacity in SK-Br3 cells, a human breast cancer cell line with high FASN expression. The compounds with an IC50 < 50 μM have been tested for their ability to inhibit FASN activity. Among them, derivative 30 blocks the 90% of FASN activity at low concentration (4 μM), is highly cytotoxic in a broad panel of tumor cells, induces apoptosis, and blocks the activation of HER2, AKT, and ERK pathways. Remarkably, 30 does not activate carnitine palmitoyltransferase-1 (CPT-1) nor induces in mice weight loss, which are the main drawbacks of other previously described FASN inhibitors. Thus, FASN inhibitor 30 may aid the validation of this enzyme as a therapeutic target for the treatment of cancer