Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization

[EN] Wheat crop production needs nitrogen (N) for ensuring yield and quality. High doses of inorganic N fertilizer are applied to soil before sowing (basal dressing), with additional doses supplied along the cultivation (top dressing). Here, a long-term wheat field trial (12 plots), including four c...

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Autores: Illescas Morente, María, Rubio Pérez, María Belén, Hernández Ruíz, Víctor, Morán Díez, María Eugenia, Martínez de Alba, Ángel Emilio, Nicolás Rodríguez, Carlos, Monte Vázquez, Enrique, Hermosa Prieto, María Rosa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168959
Acceso en línea:http://hdl.handle.net/10366/168959
Access Level:acceso abierto
Palabra clave:Bacterial composition
Fungal composition
Chemical fertilization
Bulk soil
Rhizosphere
Root Endosphere
2511.09 Microbiología de Suelos
3103.01 Producción de Cultivos
3103.03 Explotación de Los Cultivos
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spelling Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N FertilizationIllescas Morente, MaríaRubio Pérez, María BelénHernández Ruíz, VíctorMorán Díez, María EugeniaMartínez de Alba, Ángel EmilioNicolás Rodríguez, CarlosMonte Vázquez, EnriqueHermosa Prieto, María RosaBacterial compositionFungal compositionChemical fertilizationBulk soilRhizosphereRoot Endosphere2511.09 Microbiología de Suelos3103.01 Producción de Cultivos3103.03 Explotación de Los Cultivos[EN] Wheat crop production needs nitrogen (N) for ensuring yield and quality. High doses of inorganic N fertilizer are applied to soil before sowing (basal dressing), with additional doses supplied along the cultivation (top dressing). Here, a long-term wheat field trial (12 plots), including four conditions (control, N top dressing, Trichoderma harzianum T34 seed-inoculation, and top dressing plus T34) in triplicate, was performed to assess, under high basal N fertilization, the influence of these treatments on crop yield and root microbial community shaping. Crop yield was not affected by top dressing and T. harzianum T34, but top dressing significantly increased grain protein and gluten contents. Twenty-seven-week old wheat plants were collected at 12 days after top dressing application and sampled as bulk soil, rhizosphere and root endosphere compartments in order to analyze their bacterial and fungal assemblies by 16S rDNA and ITS2 high-throughput sequencing, respectively. Significant differences for bacterial and fungal richness and diversity were detected among the three compartments with a microbial decline from bulk soil to root endosphere. The most abundant wheat root phyla were Proteobacteria and Actinobacteria for bacteria, and Ascomycota and Basidiomycota for fungi. An enrichment of genera commonly associated with soils subjected to chemical N fertilization was observed: Kaistobacter, Mortierella, and Solicoccozyma in bulk soil, Olpidium in rhizosphere, and Janthinobacterium and Pedobacter in root endosphere. Taxa whose abundance significantly differed among conditions within each compartment were identified. Results show that: (i) single or strain T34-combined application of N top dressing affected to a greater extent the bulk soil bacterial levels than the use of T34 alone; (ii) when N top dressing and T34 were applied in combination, the N fertilizer played a more decisive role in the bacterial microbiome than T34; (iii) many genera of plant beneficial bacteria, negatively affected by N top dressing, were increased by the application of T34 alone; (iv) bulk soil and rhizosphere fungal microbiomes were affected by any of the three treatments assayed; and (v) all treatments reduced Claroideoglomus in bulk soil but the single application of T34 raised the rhizosphere levels of this mycorrhizal fungus.This work was supported by the Spanish Government (Project RTI2018-099986-B-I00) and the European Regional Development Fund (FEDER) under the Regional Government of Castile and Leon support (Projects SA270P18 and Escalera de Excelencia CLU-2018-04). MI was granted by a contract by Diputación de Salamanca (Projects-2018), MM-D was granted by the Program II of Postdoctoral Fellows of the University of Salamanca, and AEMA was granted with a postdoctoral contract of the Regional Government of Castile and Leon (Project SA270P18).Frontiers Media SA202620262020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/168959reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésRTI2018-099986-B-I00Projects SA270P18CLU-2018-04Projects-2018SA270P18Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1689592026-06-07T06:28:51Z
dc.title.none.fl_str_mv Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
title Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
spellingShingle Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
Illescas Morente, María
Bacterial composition
Fungal composition
Chemical fertilization
Bulk soil
Rhizosphere
Root Endosphere
2511.09 Microbiología de Suelos
3103.01 Producción de Cultivos
3103.03 Explotación de Los Cultivos
title_short Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
title_full Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
title_fullStr Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
title_full_unstemmed Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
title_sort Effect of Inorganic N Top Dressing and Trichoderma harzianum Seed-Inoculation on Crop Yield and the Shaping of Root Microbial Communities of Wheat Plants Cultivated Under High Basal N Fertilization
dc.creator.none.fl_str_mv Illescas Morente, María
Rubio Pérez, María Belén
Hernández Ruíz, Víctor
Morán Díez, María Eugenia
Martínez de Alba, Ángel Emilio
Nicolás Rodríguez, Carlos
Monte Vázquez, Enrique
Hermosa Prieto, María Rosa
author Illescas Morente, María
author_facet Illescas Morente, María
Rubio Pérez, María Belén
Hernández Ruíz, Víctor
Morán Díez, María Eugenia
Martínez de Alba, Ángel Emilio
Nicolás Rodríguez, Carlos
Monte Vázquez, Enrique
Hermosa Prieto, María Rosa
author_role author
author2 Rubio Pérez, María Belén
Hernández Ruíz, Víctor
Morán Díez, María Eugenia
Martínez de Alba, Ángel Emilio
Nicolás Rodríguez, Carlos
Monte Vázquez, Enrique
Hermosa Prieto, María Rosa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial composition
Fungal composition
Chemical fertilization
Bulk soil
Rhizosphere
Root Endosphere
2511.09 Microbiología de Suelos
3103.01 Producción de Cultivos
3103.03 Explotación de Los Cultivos
topic Bacterial composition
Fungal composition
Chemical fertilization
Bulk soil
Rhizosphere
Root Endosphere
2511.09 Microbiología de Suelos
3103.01 Producción de Cultivos
3103.03 Explotación de Los Cultivos
description [EN] Wheat crop production needs nitrogen (N) for ensuring yield and quality. High doses of inorganic N fertilizer are applied to soil before sowing (basal dressing), with additional doses supplied along the cultivation (top dressing). Here, a long-term wheat field trial (12 plots), including four conditions (control, N top dressing, Trichoderma harzianum T34 seed-inoculation, and top dressing plus T34) in triplicate, was performed to assess, under high basal N fertilization, the influence of these treatments on crop yield and root microbial community shaping. Crop yield was not affected by top dressing and T. harzianum T34, but top dressing significantly increased grain protein and gluten contents. Twenty-seven-week old wheat plants were collected at 12 days after top dressing application and sampled as bulk soil, rhizosphere and root endosphere compartments in order to analyze their bacterial and fungal assemblies by 16S rDNA and ITS2 high-throughput sequencing, respectively. Significant differences for bacterial and fungal richness and diversity were detected among the three compartments with a microbial decline from bulk soil to root endosphere. The most abundant wheat root phyla were Proteobacteria and Actinobacteria for bacteria, and Ascomycota and Basidiomycota for fungi. An enrichment of genera commonly associated with soils subjected to chemical N fertilization was observed: Kaistobacter, Mortierella, and Solicoccozyma in bulk soil, Olpidium in rhizosphere, and Janthinobacterium and Pedobacter in root endosphere. Taxa whose abundance significantly differed among conditions within each compartment were identified. Results show that: (i) single or strain T34-combined application of N top dressing affected to a greater extent the bulk soil bacterial levels than the use of T34 alone; (ii) when N top dressing and T34 were applied in combination, the N fertilizer played a more decisive role in the bacterial microbiome than T34; (iii) many genera of plant beneficial bacteria, negatively affected by N top dressing, were increased by the application of T34 alone; (iv) bulk soil and rhizosphere fungal microbiomes were affected by any of the three treatments assayed; and (v) all treatments reduced Claroideoglomus in bulk soil but the single application of T34 raised the rhizosphere levels of this mycorrhizal fungus.
publishDate 2020
dc.date.none.fl_str_mv 2020
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/168959
url http://hdl.handle.net/10366/168959
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RTI2018-099986-B-I00
Projects SA270P18
CLU-2018-04
Projects-2018
SA270P18
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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