Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey

During the last decade, the major histocompatibility complex (MHC) has received much attention in the fields of evolutionary and conservation biology because of its potential implications in many biological processes. New insights into the gene structure and evolution of MHC genes can be gained thro...

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Detalles Bibliográficos
Autores: Alcaide, Miguel, Edwards, Scott V., Negro, Juan J.
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/33948
Acceso en línea:http://hdl.handle.net/10261/33948
Access Level:acceso abierto
Palabra clave:Adaptive variation
Conservation genetics
Immunogenetics
Genetic diversity
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spelling Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of PreyAlcaide, MiguelEdwards, Scott V.Negro, Juan J.Adaptive variationConservation geneticsImmunogeneticsGenetic diversityDuring the last decade, the major histocompatibility complex (MHC) has received much attention in the fields of evolutionary and conservation biology because of its potential implications in many biological processes. New insights into the gene structure and evolution of MHC genes can be gained through study of additional lineages of birds not yet investigated at the genomic level. In this study, we characterized MHC class II B genes in five families of birds of prey (Accipitridae, Pandionidae, Strigidae, Tytonidae, and Falconidae). Using PCR approaches, we isolated genomic MHC sequences up to 1300 bp spanning exons 1 to 3 in 26 representatives of each raptor lineage, finding no stop codons or frameshift mutations in any coding region. A survey of diversity across the entirety of exon 2 in the lesser kestrel Falco naumanni reported 26 alleles in 21 individuals. Bayesian analysis revealed 21 positively selected amino acid sites, which suggests that the MHC genes described here are functional and probably expressed. Finally, through interlocus comparisons and phylogenetic analysis, we also discuss genetic evidence for concerted and transspecies evolution in the raptor MHC.Peer reviewedSpringer Nature201120112007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/33948reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.springerlink.com/content/5273m231j824926u/fulltext.pdfinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/339482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
title Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
spellingShingle Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
Alcaide, Miguel
Adaptive variation
Conservation genetics
Immunogenetics
Genetic diversity
title_short Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
title_full Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
title_fullStr Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
title_full_unstemmed Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
title_sort Characterization, Polymorphism, and Evolution of MHC Class II B Genes in Birds of Prey
dc.creator.none.fl_str_mv Alcaide, Miguel
Edwards, Scott V.
Negro, Juan J.
author Alcaide, Miguel
author_facet Alcaide, Miguel
Edwards, Scott V.
Negro, Juan J.
author_role author
author2 Edwards, Scott V.
Negro, Juan J.
author2_role author
author
dc.subject.none.fl_str_mv Adaptive variation
Conservation genetics
Immunogenetics
Genetic diversity
topic Adaptive variation
Conservation genetics
Immunogenetics
Genetic diversity
description During the last decade, the major histocompatibility complex (MHC) has received much attention in the fields of evolutionary and conservation biology because of its potential implications in many biological processes. New insights into the gene structure and evolution of MHC genes can be gained through study of additional lineages of birds not yet investigated at the genomic level. In this study, we characterized MHC class II B genes in five families of birds of prey (Accipitridae, Pandionidae, Strigidae, Tytonidae, and Falconidae). Using PCR approaches, we isolated genomic MHC sequences up to 1300 bp spanning exons 1 to 3 in 26 representatives of each raptor lineage, finding no stop codons or frameshift mutations in any coding region. A survey of diversity across the entirety of exon 2 in the lesser kestrel Falco naumanni reported 26 alleles in 21 individuals. Bayesian analysis revealed 21 positively selected amino acid sites, which suggests that the MHC genes described here are functional and probably expressed. Finally, through interlocus comparisons and phylogenetic analysis, we also discuss genetic evidence for concerted and transspecies evolution in the raptor MHC.
publishDate 2007
dc.date.none.fl_str_mv 2007
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/33948
url http://hdl.handle.net/10261/33948
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.springerlink.com/content/5273m231j824926u/fulltext.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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