CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination

CD25(+) regulatory T (T reg) cells suppress the activation/proliferation of other CD4(+) or CD8(+) T cells in vitro. Also, down-regulation of CD25(+) T reg cells enhance antitumor immune responses. In this study, we show that depletion of CD25(+) T reg cells allows the host to induce both CD4(+) and...

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Autores: Casares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7b, Arribillaga, L. (Laura)|||/items/a1a55d50-66c3-4216-9968-07b0c7f976cc, Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160, Dotor, J. (Javier)|||/items/6606b7a3-73b7-49d9-bc1b-c565b3e1c208, Lopez-Diaz-de-Cerio, A. (Ascensión)|||/items/29ab6acb-fcbb-43ff-b4c1-e2dc95e22bcb, Melero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4e, Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/20411
Acceso en línea:https://hdl.handle.net/10171/20411
Access Level:acceso abierto
Palabra clave:Angiogenesis Inhibitors/physiology
CD4-Positive T-Lymphocytes/immunology
Colonic Neoplasms/immunology
Down-Regulation/immunology
Interferon-gamma/physiology
Lymphocyte Activation/immunology
Peptides/immunology
Receptors, Interleukin-2/biosynthesis
T-Lymphocyte Subsets/immunology
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spelling CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccinationCasares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7bArribillaga, L. (Laura)|||/items/a1a55d50-66c3-4216-9968-07b0c7f976ccSarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160Dotor, J. (Javier)|||/items/6606b7a3-73b7-49d9-bc1b-c565b3e1c208Lopez-Diaz-de-Cerio, A. (Ascensión)|||/items/29ab6acb-fcbb-43ff-b4c1-e2dc95e22bcbMelero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4eLasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0cAngiogenesis Inhibitors/physiologyCD4-Positive T-Lymphocytes/immunologyColonic Neoplasms/immunologyDown-Regulation/immunologyInterferon-gamma/physiologyLymphocyte Activation/immunologyPeptides/immunologyReceptors, Interleukin-2/biosynthesisT-Lymphocyte Subsets/immunologyCD25(+) regulatory T (T reg) cells suppress the activation/proliferation of other CD4(+) or CD8(+) T cells in vitro. Also, down-regulation of CD25(+) T reg cells enhance antitumor immune responses. In this study, we show that depletion of CD25(+) T reg cells allows the host to induce both CD4(+) and CD8(+) antitumoral responses following tumor challenge. Simultaneous depletion of CD25(+) and CD8(+) cells, as well as adoptive transfer experiments, revealed that tumor-specific CD4(+) T cells, which emerged in the absence of CD25(+) T reg cells, were able to reject CT26 colon cancer cells, a MHC class II-negative tumor. The antitumoral effect mediated by CD4(+) T cells was dependent on IFN-gamma production, which exerted a potent antiangiogenic activity. The capacity of the host to mount this antitumor response is lost once the number of CD25(+) T reg cells is restored over time. However, CD25(+) T reg cell depletion before immunization with AH1 (a cytotoxic T cell determinant from CT26 tumor cells) permits the induction of a long-lasting antitumoral immune response, not observed if immunization is conducted in the presence of regulatory cells. A study of the effect of different levels of depletion of CD25(+) T reg cells before immunization with the peptide AH1 alone, or in combination with a Th determinant, unraveled that Th cells play an important role in overcoming the suppressive effect of CD25(+) T reg on the induction of long-lasting cellular immune responses.American Association of ImmunologistsDadun. Depósito Académico Digital Universidad de Navarra20122012-01-1520032003-01-0120032003-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/20411reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/204112026-06-21T12:47:57Z
dc.title.none.fl_str_mv CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
title CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
spellingShingle CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
Casares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7b
Angiogenesis Inhibitors/physiology
CD4-Positive T-Lymphocytes/immunology
Colonic Neoplasms/immunology
Down-Regulation/immunology
Interferon-gamma/physiology
Lymphocyte Activation/immunology
Peptides/immunology
Receptors, Interleukin-2/biosynthesis
T-Lymphocyte Subsets/immunology
title_short CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
title_full CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
title_fullStr CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
title_full_unstemmed CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
title_sort CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination
dc.creator.none.fl_str_mv Casares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7b
Arribillaga, L. (Laura)|||/items/a1a55d50-66c3-4216-9968-07b0c7f976cc
Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160
Dotor, J. (Javier)|||/items/6606b7a3-73b7-49d9-bc1b-c565b3e1c208
Lopez-Diaz-de-Cerio, A. (Ascensión)|||/items/29ab6acb-fcbb-43ff-b4c1-e2dc95e22bcb
Melero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4e
Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c
author Casares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7b
author_facet Casares-Lagar, N. (Noelia)|||/items/32524a9b-f393-47ee-bf77-0737c65e0b7b
Arribillaga, L. (Laura)|||/items/a1a55d50-66c3-4216-9968-07b0c7f976cc
Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160
Dotor, J. (Javier)|||/items/6606b7a3-73b7-49d9-bc1b-c565b3e1c208
Lopez-Diaz-de-Cerio, A. (Ascensión)|||/items/29ab6acb-fcbb-43ff-b4c1-e2dc95e22bcb
Melero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4e
Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c
author_role author
author2 Arribillaga, L. (Laura)|||/items/a1a55d50-66c3-4216-9968-07b0c7f976cc
Sarobe, P. (Pablo)|||/items/e6f6a7ac-cfe2-409e-ab14-057dea5fd160
Dotor, J. (Javier)|||/items/6606b7a3-73b7-49d9-bc1b-c565b3e1c208
Lopez-Diaz-de-Cerio, A. (Ascensión)|||/items/29ab6acb-fcbb-43ff-b4c1-e2dc95e22bcb
Melero, I. (Ignacio)|||/items/82113ea8-7ce1-49d5-9ee3-42cf20db1c4e
Lasarte-Sagastibelza, J.J. (Juan José)|||/items/e366ad83-3db4-41ec-a9da-ed9159ba5c0c
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Angiogenesis Inhibitors/physiology
CD4-Positive T-Lymphocytes/immunology
Colonic Neoplasms/immunology
Down-Regulation/immunology
Interferon-gamma/physiology
Lymphocyte Activation/immunology
Peptides/immunology
Receptors, Interleukin-2/biosynthesis
T-Lymphocyte Subsets/immunology
topic Angiogenesis Inhibitors/physiology
CD4-Positive T-Lymphocytes/immunology
Colonic Neoplasms/immunology
Down-Regulation/immunology
Interferon-gamma/physiology
Lymphocyte Activation/immunology
Peptides/immunology
Receptors, Interleukin-2/biosynthesis
T-Lymphocyte Subsets/immunology
description CD25(+) regulatory T (T reg) cells suppress the activation/proliferation of other CD4(+) or CD8(+) T cells in vitro. Also, down-regulation of CD25(+) T reg cells enhance antitumor immune responses. In this study, we show that depletion of CD25(+) T reg cells allows the host to induce both CD4(+) and CD8(+) antitumoral responses following tumor challenge. Simultaneous depletion of CD25(+) and CD8(+) cells, as well as adoptive transfer experiments, revealed that tumor-specific CD4(+) T cells, which emerged in the absence of CD25(+) T reg cells, were able to reject CT26 colon cancer cells, a MHC class II-negative tumor. The antitumoral effect mediated by CD4(+) T cells was dependent on IFN-gamma production, which exerted a potent antiangiogenic activity. The capacity of the host to mount this antitumor response is lost once the number of CD25(+) T reg cells is restored over time. However, CD25(+) T reg cell depletion before immunization with AH1 (a cytotoxic T cell determinant from CT26 tumor cells) permits the induction of a long-lasting antitumoral immune response, not observed if immunization is conducted in the presence of regulatory cells. A study of the effect of different levels of depletion of CD25(+) T reg cells before immunization with the peptide AH1 alone, or in combination with a Th determinant, unraveled that Th cells play an important role in overcoming the suppressive effect of CD25(+) T reg on the induction of long-lasting cellular immune responses.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01
2003
2003-01-01
2012
2012-01-15
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/20411
url https://hdl.handle.net/10171/20411
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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