Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins

Chordate Oikopleura dioica probably is the fastest evolving metazoan reported so far, and thereby, a suitable system in which to explore the limits of evolutionary processes. For this reason, and in order to gain new insights on the evolution of protein modularity, we have investigated the organizat...

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Detalles Bibliográficos
Autores: Calatayud, Sara, Garcia-Risco, Mario, Capdevila Vidal, Mercè, Cañestro García, Cristian, Palacios Bonilla, Òscar, Albalat Rodríguez, Ricard
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183505
Acceso en línea:https://hdl.handle.net/2445/183505
Access Level:acceso abierto
Palabra clave:Cordats
Evolució (Biologia)
Chordata
Evolution (Biology)
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spelling Modular Evolution and Population Variability of Oikopleura dioica MetallothioneinsCalatayud, SaraGarcia-Risco, MarioCapdevila Vidal, MercèCañestro García, CristianPalacios Bonilla, ÒscarAlbalat Rodríguez, RicardCordatsEvolució (Biologia)ChordataEvolution (Biology)Chordate Oikopleura dioica probably is the fastest evolving metazoan reported so far, and thereby, a suitable system in which to explore the limits of evolutionary processes. For this reason, and in order to gain new insights on the evolution of protein modularity, we have investigated the organization, function and evolution of multi-modular metallothionein (MT) proteins in O. dioica. MTs are a heterogeneous group of modular proteins defined by their cysteine (C)-rich domains, which confer the capacity of coordinating different transition metal ions. O. dioica has two MTs, a bi-modular OdiMT1 consisting of two domains (t-12C and 12C), and a multi-modular OdiMT2 with six t-12C/12C repeats. By means of mass spectrometry and spectroscopy of metal-protein complexes, we have shown that the 12C domain is able to autonomously bind four divalent metal ions, although the t-12C/12C pair -as it is found in OdiMT1- is the optimized unit for divalent metal binding. We have also shown a direct relationship between the number of the t-12C/12C repeats and the metal-binding capacity of the MTs, which means a stepwise mode of functional and structural evolution for OdiMT2. Finally, after analyzing four different O. dioica populations worldwide distributed, we have detected several OdiMT2 variants with changes in their number of t-12C/12C domain repeats. This finding reveals that the number of repeats fluctuates between current O. dioica populations, which provides a new perspective on the evolution of domain repeat proteins.Frontiers Media2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/183505Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fcell.2021.702688Frontiers In Cell And Developmental Biology, 2021, vol. 9, p. 702688https://doi.org/10.3389/fcell.2021.702688cc-by (c) Calatayud, Sara et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1835052026-05-29T05:05:01Z
dc.title.none.fl_str_mv Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
title Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
spellingShingle Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
Calatayud, Sara
Cordats
Evolució (Biologia)
Chordata
Evolution (Biology)
title_short Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
title_full Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
title_fullStr Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
title_full_unstemmed Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
title_sort Modular Evolution and Population Variability of Oikopleura dioica Metallothioneins
dc.creator.none.fl_str_mv Calatayud, Sara
Garcia-Risco, Mario
Capdevila Vidal, Mercè
Cañestro García, Cristian
Palacios Bonilla, Òscar
Albalat Rodríguez, Ricard
author Calatayud, Sara
author_facet Calatayud, Sara
Garcia-Risco, Mario
Capdevila Vidal, Mercè
Cañestro García, Cristian
Palacios Bonilla, Òscar
Albalat Rodríguez, Ricard
author_role author
author2 Garcia-Risco, Mario
Capdevila Vidal, Mercè
Cañestro García, Cristian
Palacios Bonilla, Òscar
Albalat Rodríguez, Ricard
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cordats
Evolució (Biologia)
Chordata
Evolution (Biology)
topic Cordats
Evolució (Biologia)
Chordata
Evolution (Biology)
description Chordate Oikopleura dioica probably is the fastest evolving metazoan reported so far, and thereby, a suitable system in which to explore the limits of evolutionary processes. For this reason, and in order to gain new insights on the evolution of protein modularity, we have investigated the organization, function and evolution of multi-modular metallothionein (MT) proteins in O. dioica. MTs are a heterogeneous group of modular proteins defined by their cysteine (C)-rich domains, which confer the capacity of coordinating different transition metal ions. O. dioica has two MTs, a bi-modular OdiMT1 consisting of two domains (t-12C and 12C), and a multi-modular OdiMT2 with six t-12C/12C repeats. By means of mass spectrometry and spectroscopy of metal-protein complexes, we have shown that the 12C domain is able to autonomously bind four divalent metal ions, although the t-12C/12C pair -as it is found in OdiMT1- is the optimized unit for divalent metal binding. We have also shown a direct relationship between the number of the t-12C/12C repeats and the metal-binding capacity of the MTs, which means a stepwise mode of functional and structural evolution for OdiMT2. Finally, after analyzing four different O. dioica populations worldwide distributed, we have detected several OdiMT2 variants with changes in their number of t-12C/12C domain repeats. This finding reveals that the number of repeats fluctuates between current O. dioica populations, which provides a new perspective on the evolution of domain repeat proteins.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183505
url https://hdl.handle.net/2445/183505
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fcell.2021.702688
Frontiers In Cell And Developmental Biology, 2021, vol. 9, p. 702688
https://doi.org/10.3389/fcell.2021.702688
dc.rights.none.fl_str_mv cc-by (c) Calatayud, Sara et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Calatayud, Sara et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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