Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays

Angiogenesis is a highly ordered physiological process regulated by the interaction of endothelial cells with an extensive variety of growth factors, extracellular matrix components and mechanical stimuli. One of the most important challenges in tissue engineering is the rapid neovascularization of...

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Autores: Balcells, Mercedes, Claeyssens, Frederik, Dikici, Serkan, Dikici, Betül Aldemir, Bhaloo, Shirin Issa, Edelman, Elazer R., MacNeil, Sheila, Reilly, Gwendolen C., Sherborne, Colin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/1123
Acceso en línea:http://hdl.handle.net/20.500.14342/1123
https://doi.org/10.3389/fbioe.2019.00451
Access Level:acceso abierto
Palabra clave:Endoteli vascular
Angiogenesis
Vascular endothelial growth factor (VEGF)
2-deoxy-D-ribose (2dDR)
Thymidine phosphorylase (TP)
Angiogenesis assays
Shear stress
Emulsion templating
PolyHIPE
57
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spelling Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assaysBalcells, MercedesClaeyssens, FrederikDikici, SerkanDikici, Betül AldemirBhaloo, Shirin IssaEdelman, Elazer R.MacNeil, SheilaReilly, Gwendolen C.Sherborne, ColinEndoteli vascularAngiogenesisVascular endothelial growth factor (VEGF)2-deoxy-D-ribose (2dDR)Thymidine phosphorylase (TP)Angiogenesis assaysShear stressEmulsion templatingPolyHIPE57Angiogenesis is a highly ordered physiological process regulated by the interaction of endothelial cells with an extensive variety of growth factors, extracellular matrix components and mechanical stimuli. One of the most important challenges in tissue engineering is the rapid neovascularization of constructs to ensure their survival after transplantation. To achieve this, the use of pro-angiogenic agents is a widely accepted approach. The study of angiogenesis has gained momentum over the last two decades. Although there are various in vitro, ex vivo, and in vivo angiogenesis models that enable testing of newly discovered pro-angiogenic agents, the problem with researching angiogenesis is the choice of the most appropriate assay. In vivo assays are the most representative and reliable models, but they are expensive, time-consuming and can cause ethical concerns whereas in vitro assays are relatively inexpensive, practical, and reproducible, but they are usually lack of enabling the study of more than one aspect of angiogenesis, and they do not fully represent the complexity of physiological angiogenesis. Therefore, there is a need for the development of an angiogenesis model that allows the study of angiogenesis under physiologically more relevant, dynamic conditions without causing ethical concerns. Accordingly, in this study, we developed 3D in vitro dynamic angiogenesis model, and we tested the angiogenic potential of 2-deoxy-D-ribose (2dDR) in comparison with vascular endothelial growth factor (VEGF) using newly developed in vitro 3D dynamic model and well-established in vitro models. Our results obtained using conventional in vitro assays demonstrated that 2dDR promoted proliferation, migration and tube formation of human aortic endothelial cells (HAECs) in a dose-dependent manner. Then, the angiogenic activity of 2dDR was further assessed using the newly developed 3D in vitro model, which enabled the monitoring of cell proliferation and infiltration simultaneously under dynamic conditions. Our results showed that the administration of 2dDR and VEGF significantly enhanced the outgrowth of HAECs and the cellular density under either static or dynamic conditions.Frontiers MediaUniversitat Ramon Llull. IQS2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion20 p.http://hdl.handle.net/20.500.14342/1123https://doi.org/10.3389/fbioe.2019.00451reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in Bioengineering and Biotechnology. Vol.7 (2020), 451​info:eu-repo/grantAgreement/EPSRC/EP/I007695/1​info:eu-repo/grantAgreement/MRC/MR/L012669/1info:eu-repo/grantAgreement/MINECO/PN I+D/SAF2017-84773-C2-1-Rinfo:eu-repo/grantAgreement/US National Institute of Health/R01 49039/Attribution 4.0 International© L'autor/ahttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/11232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
title Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
spellingShingle Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
Balcells, Mercedes
Endoteli vascular
Angiogenesis
Vascular endothelial growth factor (VEGF)
2-deoxy-D-ribose (2dDR)
Thymidine phosphorylase (TP)
Angiogenesis assays
Shear stress
Emulsion templating
PolyHIPE
57
title_short Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
title_full Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
title_fullStr Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
title_full_unstemmed Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
title_sort Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
dc.creator.none.fl_str_mv Balcells, Mercedes
Claeyssens, Frederik
Dikici, Serkan
Dikici, Betül Aldemir
Bhaloo, Shirin Issa
Edelman, Elazer R.
MacNeil, Sheila
Reilly, Gwendolen C.
Sherborne, Colin
author Balcells, Mercedes
author_facet Balcells, Mercedes
Claeyssens, Frederik
Dikici, Serkan
Dikici, Betül Aldemir
Bhaloo, Shirin Issa
Edelman, Elazer R.
MacNeil, Sheila
Reilly, Gwendolen C.
Sherborne, Colin
author_role author
author2 Claeyssens, Frederik
Dikici, Serkan
Dikici, Betül Aldemir
Bhaloo, Shirin Issa
Edelman, Elazer R.
MacNeil, Sheila
Reilly, Gwendolen C.
Sherborne, Colin
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Endoteli vascular
Angiogenesis
Vascular endothelial growth factor (VEGF)
2-deoxy-D-ribose (2dDR)
Thymidine phosphorylase (TP)
Angiogenesis assays
Shear stress
Emulsion templating
PolyHIPE
57
topic Endoteli vascular
Angiogenesis
Vascular endothelial growth factor (VEGF)
2-deoxy-D-ribose (2dDR)
Thymidine phosphorylase (TP)
Angiogenesis assays
Shear stress
Emulsion templating
PolyHIPE
57
description Angiogenesis is a highly ordered physiological process regulated by the interaction of endothelial cells with an extensive variety of growth factors, extracellular matrix components and mechanical stimuli. One of the most important challenges in tissue engineering is the rapid neovascularization of constructs to ensure their survival after transplantation. To achieve this, the use of pro-angiogenic agents is a widely accepted approach. The study of angiogenesis has gained momentum over the last two decades. Although there are various in vitro, ex vivo, and in vivo angiogenesis models that enable testing of newly discovered pro-angiogenic agents, the problem with researching angiogenesis is the choice of the most appropriate assay. In vivo assays are the most representative and reliable models, but they are expensive, time-consuming and can cause ethical concerns whereas in vitro assays are relatively inexpensive, practical, and reproducible, but they are usually lack of enabling the study of more than one aspect of angiogenesis, and they do not fully represent the complexity of physiological angiogenesis. Therefore, there is a need for the development of an angiogenesis model that allows the study of angiogenesis under physiologically more relevant, dynamic conditions without causing ethical concerns. Accordingly, in this study, we developed 3D in vitro dynamic angiogenesis model, and we tested the angiogenic potential of 2-deoxy-D-ribose (2dDR) in comparison with vascular endothelial growth factor (VEGF) using newly developed in vitro 3D dynamic model and well-established in vitro models. Our results obtained using conventional in vitro assays demonstrated that 2dDR promoted proliferation, migration and tube formation of human aortic endothelial cells (HAECs) in a dose-dependent manner. Then, the angiogenic activity of 2dDR was further assessed using the newly developed 3D in vitro model, which enabled the monitoring of cell proliferation and infiltration simultaneously under dynamic conditions. Our results showed that the administration of 2dDR and VEGF significantly enhanced the outgrowth of HAECs and the cellular density under either static or dynamic conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/1123
https://doi.org/10.3389/fbioe.2019.00451
url http://hdl.handle.net/20.500.14342/1123
https://doi.org/10.3389/fbioe.2019.00451
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Bioengineering and Biotechnology. Vol.7 (2020), 451
​info:eu-repo/grantAgreement/EPSRC/EP/I007695/1
​info:eu-repo/grantAgreement/MRC/MR/L012669/1
info:eu-repo/grantAgreement/MINECO/PN I+D/SAF2017-84773-C2-1-R
info:eu-repo/grantAgreement/US National Institute of Health/R01 49039/
dc.rights.none.fl_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20 p.
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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