Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes

Liposomes containing theobromine, caffeine, catechin, epicatechin, and a cocoa extract were fabricated using microfluidization and sonication. A high encapsulation efficiency and good physicochemical stability were obtained by sonication (75% amplitude, 7 min). Liposomes produced at pH 5.0 had mean...

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Detalles Bibliográficos
Autores: Toro-Uribe, Said, Ibáñez, Elena, Decker, Eric A., McClements, David Julian, Zhang, Ruojie, López-Giraldo, Luis, Herrero, Miguel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/192187
Acceso en línea:http://hdl.handle.net/10261/192187
Access Level:acceso abierto
Palabra clave:Alkaloids
Catechins
Cocoa polyphenols
Liposomes
In vitro digestion
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spelling Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomesToro-Uribe, SaidIbáñez, ElenaDecker, Eric A.McClements, David JulianZhang, RuojieLópez-Giraldo, LuisHerrero, MiguelAlkaloidsCatechinsCocoa polyphenolsLiposomesIn vitro digestionLiposomes containing theobromine, caffeine, catechin, epicatechin, and a cocoa extract were fabricated using microfluidization and sonication. A high encapsulation efficiency and good physicochemical stability were obtained by sonication (75% amplitude, 7 min). Liposomes produced at pH 5.0 had mean particle diameter ranging from 73.9 to 84.3 nm. The structural and physicochemical properties of the liposomes were characterized by transmission electron microscopy, confocal fluorescence microscopy, and antioxidant activity assays. The release profile was measured by ultra-high performance liquid chromatography coupled to diode array detection. The bioaccessibility of the bioactive compounds encapsulated in liposomes was determined after exposure to a simulated in vitro digestion model. Higher bioaccessibilities were measured for all catechins-loaded liposome formulations as compared to nonencapsulated counterparts. These results demonstrated that liposomes are capable of increasing the bioaccessibility of flavan-3-ols, which may be important for the development of nutraceutical-enriched functional foods.S.T.-U. wants to thank Colciencias-Colombia for financial support, and Universidad Industrial of Santander (Colombia), University of Massachusetts (Food Science Department, MA), and Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, Spain) for making this work possible. We thank Project AGL2017-89417-R (MINECO, Spain) for financial support.Peer reviewedAmerican Chemical SocietyAgencia Estatal de Investigación (España)Ministerio de Economía y Competitividad (España)Colciencias (Colombia)Universidad Industrial de Santander (Colombia)CSIC-UAM - Instituto de Investigación en Ciencias de la Alimentación (CIAL)University of MassachusettsMinisterio de Ciencia, Innovación y Universidades (España)Toro-Uribe, Said [0000-0002-7107-7123]McClements, David Julian [0000-0002-9016-1291]Herrero, Miguel [0000-0002-7214-6653]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/192187reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#AGL2017-89417-R/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-89417-Rhttps://doi.org/10.1021/acs.jafc.8b04735Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1921872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
title Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
spellingShingle Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
Toro-Uribe, Said
Alkaloids
Catechins
Cocoa polyphenols
Liposomes
In vitro digestion
title_short Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
title_full Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
title_fullStr Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
title_full_unstemmed Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
title_sort Design, fabrication, characterization, and in vitro digestion of alkaloid-, catechin-, and cocoa extract-loaded liposomes
dc.creator.none.fl_str_mv Toro-Uribe, Said
Ibáñez, Elena
Decker, Eric A.
McClements, David Julian
Zhang, Ruojie
López-Giraldo, Luis
Herrero, Miguel
author Toro-Uribe, Said
author_facet Toro-Uribe, Said
Ibáñez, Elena
Decker, Eric A.
McClements, David Julian
Zhang, Ruojie
López-Giraldo, Luis
Herrero, Miguel
author_role author
author2 Ibáñez, Elena
Decker, Eric A.
McClements, David Julian
Zhang, Ruojie
López-Giraldo, Luis
Herrero, Miguel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Economía y Competitividad (España)
Colciencias (Colombia)
Universidad Industrial de Santander (Colombia)
CSIC-UAM - Instituto de Investigación en Ciencias de la Alimentación (CIAL)
University of Massachusetts
Ministerio de Ciencia, Innovación y Universidades (España)
Toro-Uribe, Said [0000-0002-7107-7123]
McClements, David Julian [0000-0002-9016-1291]
Herrero, Miguel [0000-0002-7214-6653]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Alkaloids
Catechins
Cocoa polyphenols
Liposomes
In vitro digestion
topic Alkaloids
Catechins
Cocoa polyphenols
Liposomes
In vitro digestion
description Liposomes containing theobromine, caffeine, catechin, epicatechin, and a cocoa extract were fabricated using microfluidization and sonication. A high encapsulation efficiency and good physicochemical stability were obtained by sonication (75% amplitude, 7 min). Liposomes produced at pH 5.0 had mean particle diameter ranging from 73.9 to 84.3 nm. The structural and physicochemical properties of the liposomes were characterized by transmission electron microscopy, confocal fluorescence microscopy, and antioxidant activity assays. The release profile was measured by ultra-high performance liquid chromatography coupled to diode array detection. The bioaccessibility of the bioactive compounds encapsulated in liposomes was determined after exposure to a simulated in vitro digestion model. Higher bioaccessibilities were measured for all catechins-loaded liposome formulations as compared to nonencapsulated counterparts. These results demonstrated that liposomes are capable of increasing the bioaccessibility of flavan-3-ols, which may be important for the development of nutraceutical-enriched functional foods.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/192187
url http://hdl.handle.net/10261/192187
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
AGL2017-89417-R/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-89417-R
https://doi.org/10.1021/acs.jafc.8b04735
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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