Wnt pathway activation in long term remnant rat model

Progression of chronic kidney disease (CKD) is characterized by deposition of extracellular matrix. This is an irreversible process that leads to tubulointerstitial fibrosis and finally loss of kidney function. Wnt/ ?-catenin pathway was reported to be aberrantly activated in the progressive damage...

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Autores: Bañón Maneus, Elisenda, Rovira Juárez, Jordi, Ramírez Bajo, María José, Moya Rull, Daniel, Hierro García, Natalia, Takenaka, S, Diekmann, Fritz, Eickelberg, O, Konigshoff, M, Campistol Plana, Josep M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Oviedo (UNIOVI)
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/224368
Acceso en línea:https://hdl.handle.net/2445/224368
Access Level:acceso abierto
Palabra clave:Insuficiència renal crònica
Glomèruls renals
Aparell urinari
Chronic renal failure
Kidney glomerulus
Urinary organs
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spelling Wnt pathway activation in long term remnant rat modelBañón Maneus, ElisendaRovira Juárez, JordiRamírez Bajo, María JoséMoya Rull, DanielHierro García, NataliaTakenaka, SDiekmann, FritzEickelberg, OKonigshoff, MCampistol Plana, Josep M.Insuficiència renal crònicaGlomèruls renalsAparell urinariChronic renal failureKidney glomerulusUrinary organsProgression of chronic kidney disease (CKD) is characterized by deposition of extracellular matrix. This is an irreversible process that leads to tubulointerstitial fibrosis and finally loss of kidney function. Wnt/ ?-catenin pathway was reported to be aberrantly activated in the progressive damage associated with chronic organ failure. Extensive renal ablation is an experimental model widely used to gain insight into the mechanisms responsible for the development of CKD, but it was not evaluated for Wnt/ ?-catenin pathway. This study aimed to elucidate if the rat 5/6 renal mass reduction model (RMR) is a good model for the Wnt/ ?-catenin activation and possible next modulation. RMR model was evaluated at 12 and 18 weeks after the surgery, when CKD is close to end-stage kidney disease demonstrated by molecular and histological studies. Wnt pathway components were analyzed at mRNA and protein level. Our results demonstrate that Wnt pathway is active by increase of ?-catenin at mRNA level and nuclear translocation in tubular epithelium as well as some target genes. These results validate the RMR model for future modulation of Wnt pathway, starting at shorter time after the surgery.Hindawi Publishing Corporation2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/224368Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)reponame:Dipòsit Digital de la UBinstname:Universidad de Oviedo (UNIOVI)InglésReproducció del document publicat a: https://doi.org/10.1155/2014/324713Biomed Research International, vol. 2014, 324713https://doi.org/10.1155/2014/324713cc-by (c) Bañón Maneus, Elisenda et al., 2025https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2243682026-05-27T06:46:51Z
dc.title.none.fl_str_mv Wnt pathway activation in long term remnant rat model
title Wnt pathway activation in long term remnant rat model
spellingShingle Wnt pathway activation in long term remnant rat model
Bañón Maneus, Elisenda
Insuficiència renal crònica
Glomèruls renals
Aparell urinari
Chronic renal failure
Kidney glomerulus
Urinary organs
title_short Wnt pathway activation in long term remnant rat model
title_full Wnt pathway activation in long term remnant rat model
title_fullStr Wnt pathway activation in long term remnant rat model
title_full_unstemmed Wnt pathway activation in long term remnant rat model
title_sort Wnt pathway activation in long term remnant rat model
dc.creator.none.fl_str_mv Bañón Maneus, Elisenda
Rovira Juárez, Jordi
Ramírez Bajo, María José
Moya Rull, Daniel
Hierro García, Natalia
Takenaka, S
Diekmann, Fritz
Eickelberg, O
Konigshoff, M
Campistol Plana, Josep M.
author Bañón Maneus, Elisenda
author_facet Bañón Maneus, Elisenda
Rovira Juárez, Jordi
Ramírez Bajo, María José
Moya Rull, Daniel
Hierro García, Natalia
Takenaka, S
Diekmann, Fritz
Eickelberg, O
Konigshoff, M
Campistol Plana, Josep M.
author_role author
author2 Rovira Juárez, Jordi
Ramírez Bajo, María José
Moya Rull, Daniel
Hierro García, Natalia
Takenaka, S
Diekmann, Fritz
Eickelberg, O
Konigshoff, M
Campistol Plana, Josep M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Insuficiència renal crònica
Glomèruls renals
Aparell urinari
Chronic renal failure
Kidney glomerulus
Urinary organs
topic Insuficiència renal crònica
Glomèruls renals
Aparell urinari
Chronic renal failure
Kidney glomerulus
Urinary organs
description Progression of chronic kidney disease (CKD) is characterized by deposition of extracellular matrix. This is an irreversible process that leads to tubulointerstitial fibrosis and finally loss of kidney function. Wnt/ ?-catenin pathway was reported to be aberrantly activated in the progressive damage associated with chronic organ failure. Extensive renal ablation is an experimental model widely used to gain insight into the mechanisms responsible for the development of CKD, but it was not evaluated for Wnt/ ?-catenin pathway. This study aimed to elucidate if the rat 5/6 renal mass reduction model (RMR) is a good model for the Wnt/ ?-catenin activation and possible next modulation. RMR model was evaluated at 12 and 18 weeks after the surgery, when CKD is close to end-stage kidney disease demonstrated by molecular and histological studies. Wnt pathway components were analyzed at mRNA and protein level. Our results demonstrate that Wnt pathway is active by increase of ?-catenin at mRNA level and nuclear translocation in tubular epithelium as well as some target genes. These results validate the RMR model for future modulation of Wnt pathway, starting at shorter time after the surgery.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/224368
url https://hdl.handle.net/2445/224368
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1155/2014/324713
Biomed Research International, vol. 2014, 324713
https://doi.org/10.1155/2014/324713
dc.rights.none.fl_str_mv cc-by (c) Bañón Maneus, Elisenda et al., 2025
https://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Bañón Maneus, Elisenda et al., 2025
https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
reponame:Dipòsit Digital de la UB
instname:Universidad de Oviedo (UNIOVI)
instname_str Universidad de Oviedo (UNIOVI)
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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